Yazar "Burgu, Ibrahim" seçeneğine göre listele

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    First molecular characterization of feline immunodeficiency virus in Turkey
    (Springer Wien, 2010) Oguzoglu, Tuba Cigdem; Timurkan, Mehmet Ozkan; Muz, Dilek; Kudu, Aysegul; Numanbayraktaroglu, Basak; Sadak, Seda; Burgu, Ibrahim
    In this study, strains of feline immunodeficiency virus (Fly), designated TR-D, TR-Mo and TR-Mi, isolated from three cats in Turkey, were characterized. PCR products (859 bp) from the envelope (env) gene region were amplified and sequenced, and possible geographical differences in the env gene region of Turkish FIV strains are discussed. Phylogenetic analysis of two strains showed that FIV subtype B was present in Turkey. Phylogenetic analysis showed that one new Turkish FIV strain occupies a separate branch from known clusters (subtypes A to E) from the USA, Canada, Europe and Japan.
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    First molecular characterization of visna/maedi viruses from naturally infected sheep in Turkey
    (Springer Wien, 2013) Muz, Dilek; Oguzoglu, Tuba Cigdem; Rosati, Sergio; Reina, Ramses; Bertolotti, Luigi; Burgu, Ibrahim
    Recent worldwide serological and genetic studies of small ruminant lentiviruses (SRLV) have led to the description of new genotypes and the development of new diagnostic tests. This study investigated the detection and molecular characterization of visna/maedi virus (VMV) infection in serum and blood samples from pure and mixed sheep breeds acquired from different regions in Turkey using ELISA and PCR techniques. The prevalence of VMV was 67.8 % by ELISA and/or LTR-PCR with both assays showing a medium level of agreement (kappa: 0.26; +/- A 0.038 CI). Positivity of VMV in sheep increased according to the age of the animal, although PCR positivity was higher than ELISA in young individuals. Phylogenetic analysis of 33 LTR sequences identified two distinct clades that were closely related to American and Greek LTR sequences. Phylogenetic analysis of 10 partial gag gene sequences identified A2, A3, A5, A9, A11 subtypes of genotype A SRLVs. In vitro culture of all isolates in fetal sheep lung cells (FSLC) showed a slow/low phenotype causing less or no lytic infection compared with infection with the WLC-1 American strain characterized by a rapid/highly lytic phenotype. Phylogenetic analysis revealed that Turkish VMV sequences preceded the establishment of American or Greek strains that were associated with the migration of sheep from the Middle East to Western Europe several centuries ago. This is the first study that describes Turkish VMV sequences with the molecular characterization of LTR and gag genes, and it strongly suggests that SRLV-genotype A originated in Turkey.
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    Molecular characterization of Bovine virus diarrhea viruses species 2 (BVDV-2) from cattle in Turkey
    (Springer, 2010) Oguzoglu, Tuba Cigdem; Muz, Dilek; Yilmaz, Volkan; Alkan, Feray; Akca, Yilmaz; Burgu, Ibrahim
    Five BVDV species 2 (BVDV-2) isolates were detected from cattle in Turkey. Phylogenetic analysis was performed based on the 5' untranslated regions (UTRs) and E2 coding gene regions, respectively. The isolates were closely related to BVDV-2a strains from North America and Canada used as references. This is the first report of the detection of BVDV-2 in naturally infected Turkish cattle. It is important to consider BVDV-2 for planning future BVDV control and vaccination programs in Turkey.