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    ASSOCIATION OF FETUIN-A WITH GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND OXIDATIVE STRESS IN SICKLE CELL ANEMIA
    (Carbone Editore, 2016) Ulutas, Kemal Turker; Buyukbas, Sadik; Yilmaz, Nigar; Arpaci, Abdullah; Kaya, Hasan
    Aim: Oxidative stress has crucial effects over vascular pathophysiology of Sickle Cell Anemia (SCA), and contributes atherosclerosis at advanced stages of the disease. In contrast to be acknowledged the possible role of Fetuin-A in the development of atherosclerosis, its association with vascular changes in SCA appears to warrant further investigation. In the study, we aimed to illuminate vascular pathophysiology of SCA via investigating Fetuin-A levels and its relationship with oxidative stress parameters. Material and methods: Fourty SCA patients and and 35 healthy individuals (n: 35) were included in the study all. After analyzing hematological data, Malondialdehyde (MDA), Glutathione Peroxidase (GSH-Px), Superoxide Dismutase (SOD) and Catalase (CAT) were measured throughout preparing hemolysate from samples with EDTA. Fetuin-A was analyzed from serum samples. Results: Fetuin-A (p<0.001) and MDA levels (p<0.001) were measured significantly higher in SCA group. Similarly, G6PD (p<0.001) and SOD (p<0.01) enzyme activities were determined higher in SCA when compared to the control group. A novel positive correlation was found among Fetuin-A, G6PD (r=0.435, p<0.0001) and MDA (r=0.547, p<0.0001). However, there was no statistical significance between the groups for CAT and GSH-Px. ROC analyze suggested 0,97 ng/mL as the cutoff value for Fetuin-A (Area Under the Curve: 0.713, sensitivity: 62%, specificity: 80%, p:0,002) and 0,06 mu mol/gr Hb as the cutoff value for MDA (Area Under the Curve: 0.898, sensitivity: 87 %, specificity: 80%, p:<0,001). Conclusion: Elevated Fetuin-A in patients with SCA and its positive correlations with both MDA and G6PD suggested that Fetuin-A might have an important role for pathophysiology of SCA. Additionally, Fetuin-A may be useful for evaluating severity of disease with high specificity cutoff value as MDA.
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    The Effects of Caffeic Acid Phenethyl Ester and Ellagic Acid on the Levels of Malondialdehyde, Reduced Glutathione and Nitric Oxide in the Lung, Liver and Kidney Tissues in Acute Diazinon Toxicity in Rats
    (Medwell Online, 2011) Alp, Harun; Aytekin, Ismail; Atakisi, Onur; Hatipoglu, Namik Kemal; Basarali, Kemal; Ogun, Metin; Buyukbas, Sadik
    The aim of this study was to investigate the effects of Caffeic Acid Phenethyl Ester (CAPE) and Ellagic Acid (EA) on acitivities of Malondialdehyde (MDA), reduced Glutathione (GSH) and Nitric Oxide (NO) in rat lung, liver and kidney tissues in acute Diazinon (DI) toxicity. Six groups of 6 Sprague Dawley rats were used comprising control, CAPE, EA, DI control, DI+CAPE and DI+EA. Tissue samples were analysed for GSH, MDA and NO levels in lung, liver and kidney tissues. Biochemical parameters were measured colormetrically by spectrophotometer. Control, CAPE and EA groups showed no statistically significant difference whereas DI+medication groups revealed that CAPE and EA increased the level of GSH in liver tissue by blocking the DI effect. NO levels in lung, liver and kidney tissues were significantly increased by DI but CAPE and EA attenuated those levels. In DI+medication groups, MDA levels showed no significant change in kidney and liver tissues but in lung tissues, CAPE and EA reduced the MDA level by blocking the DI effect. It was concluded that CAPE and EA which showed similar effects to each other could be used for protection and support against oxidative stress caused by acute DI intoxication.
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    Effects of Silybum marianum Extract on High-Fat Diet Induced Metabolic Disorders in Rats
    (Inst Animal Reproduction & Food Research Polish Acad Sciences Olsztyn, 2016) Sayin, Fatma Kubra; Buyukbas, Sadik; Basarali, M. Kemal; Alp, Harun; Toy, Hatice; Ugurcu, Veli
    Silybum marianum extract (SME) has been used for centuries as a natural remedy for diseases of liver and biliary tract. Lately, it has been promoted as a nutritional supplement for beneficial effects on some risk factors of diabetes and hyperlipidemia. In this study we aimed to determine the effects of SME on high-fat diet (HFD) induced metabolic disorders. Male Sprague Dawley rats were fed HFD for 11 weeks to induce obesity. SME was given to animals for two different durations, for 11 weeks or for 7 weeks. The results showed significant increase in plasma transaminases, total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C), leptin, high sensitive C-reactive protein (hsCRP), glucose and insulin along with significant increase in body mass index (BMI) and liver weights in rats fed the HFD diet compared to rats fed with standard rat diet. SME supplementation for different durations raised improvement in the HFD-induced metabolic disorders such as insulin resistance, hyperlipidemia and hepatopathy at different degrees. Our study concludes that SME can be well considered as an effective supplement to improve insulin and leptin sensitivity and hyperlipidemia and to suppress body weight gain.