Yazar "Caglayan, K" seçeneğine göre listele

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    Öğe
    Characterization of Plum pox potyvirus (PPV) by DAS-ELISA and RT-PCR/RFLP analysis in Turkey
    (Int Soc Horticultural Science, 2004) Caglayan, K; Sertkaya, G; Ulubas, C; Kölber, M; Krizbai, L
    This study was conducted to determine the presence of Plum pox virus (PPV) (family Potyviridae, genus Potyvirus) in different regions of Turkey and to characterise PPV isolates by serological and molecular techniques including ELISA, PCR/ RFLP. Thus, leaf samples from different stone fruit species, almond, apricot, nectarine, peach, plum, sour and sweet cherry exhibiting various types of symptoms related to PPV were collected from different parts of the canopy from randomly selected orchards in the main stone fruit growing areas in Turkey. Polyclonal antibodies (PAbs) were used to detect the presence of PPV in the plant samples by serological assays (DAS-ELISA). The following monoclonal antibodies (Mabs): Mab5B (Universal), Mab4DG5 (PPV-D: Dideron-specific), MabAL (PPV-M: Marcus-specific), MabEA24 (PPV-El Amar-specific) and MabAC (PPV-C: Cherry-specific) were used to identify the serotyping of PPV isolates. Reverse transcription-polymerase chain reaction (RT-PCR) assays and restriction fragment length polymorphism (RFLP) analysis of RT-PCR products were performed to characterize Turkish PPV isolates. The results of RT-PCR analyses using general primers were in complete agreement with the DAS-ELISA and DASI-ELISA results, showing that 2 apricot samples of 52 stone fruit samples collected from Ankara province were found to be infected with M strain of PPV. This study confirmed the results of the previous work demonstrated the presence of PPV-M strain on apricot in Turkey.
  • [ N/A ]
    Öğe
    Detection of some viruses of stone fruits in mother plant blocks in eastern Mediterranean region of Turkey
    (Int Soc Horticultural Science, 2004) Sertkaya, G; Caglayan, K; Ulubas, C
    Field inspection and sample collection were carried out in mother plant blocks which are also varietal collection belonging to the Ministry of Agriculture and the Universities in Eastern Mediterranean Region of Turkey during early spring and in autumn in 2002. Samples (shoots and leaves) were collected from symptomatic plants in the orchards. 48 samples (3 almond, 9 apricot, 3 nectarine, 10 peach, It plum, 12 sweet cherry) having different symptoms were collected and tested for the presence of Apple chlorotic leaf spot trichovirus (ACLSV), Apple mosaic ilarvirus (ApMV), Cherry leaf roll nepovirus (CLRV), Prune dwarf ilarvirus (PDV), Prunus necrotic ringspot ilarvirus (PNRSV) and Plum pox potyvirus (PPV) by using DAS-ELISA. According to serological assays, the most common virus was PDV. It was followed by PNRSV and ACLSV. The reverse transcription-polymerase chain reaction (RT-PCR) assays were performed for investigation of PDV, PNRSV and PPV in the samples. The following viruses were identified by ELISA and RT-PCR assays: ACLSV (1 sample), CLRV (1 sample), PDV (5 samples), PNRSV (2 samples). Mix infections of ACLSV+PDV (1 sample) and PDV+PNRSV (2 samples) were also found. No PPV-infected samples were detected from the samples collected from mother plant blocks in Eastern Mediterranean Region of Turkey.
  • [ N/A ]
    Öğe
    First report of olive viruses in Turkey
    (Edizioni Ets, 2004) Caglayan, K; Fidan, U; Tarla, G; Gazel, M
    [Abstract Not Available]
  • Küçük Resim
    Öğe
    Virus and virus-like diseases of stone fruits in the eastern Mediterranean area of Turkey
    (International Society Horticultural Science, 1998) Caglayan, K; Gazel, MH
    Stone fruit trees were surveyed for the presence of virus and virus-like diseases in the eastern Mediterranean area of Turkey. The main observed symptoms of different viruses were dwarfing, shortened internodes, bushy shoots, necrotic and chlorotic spots on leaves, yellowing, line pattern, vein banding and fruit deformation in all provinces surveyed. Viruses identified were prunus necrotic ring spot virus (PNRSV), prune dwarf virus (PDV), apple mosaic virus (ApMV) and apple chlorotic leaf spot virus (ACLSV). ELISA tests showed that 28.4 % of 474 stone fruit trees individually checked were infected with one or more viruses. The most common virus was ApMV, which had a mean infection rate of 22.2 %. It was followed by PNRSV, PDV and ACLSV with a mean infection rate of 17.1 %, 15.5 %, and 13.3 %, respectively. Plum pox virus was not detected in all of the tested samples.