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    Association between Ala-9Val polymorphism of Mn-SOD gene and schizophrenia
    (Pergamon-Elsevier Science Ltd, 2005) Akyol, O; Yanik, M; Elyas, H; Namli, M; Canatan, H; Akin, H; Yuce, H
    Reactive oxygen species (ROS) have been suggested to play an important role in physiopathology of schizophrenia. The major intracellular antioxidant enzymes, copper-zinc superoxide dismutase in the cytoplasm and manganese superoxide dismutase (Mn-SOD) in the mitochondria, rapidly and specifically reduce superoxide radicals to hydrogen peroxide. Polymorphisms in the genes encoding antioxidant enzymes should therefore result in predisposition to schizophrenia. The present study was performed to assess; whether there is a genetic association between a functional polymorphism (Ala-9Val) in the human Mn-SOD gene in schizophrenic patients (n=153) and healthy controls (n=196) using a PCR/RFLP method. Significant differences in the genotypic distribution between schizophrenics and controls were observed. Genotypic distribution with 14 (9.2%) Ala/Ala, 106 (69.3%) Ala/Val and 33 (21.6%) Val/Val subjects, in schizophrenia was different from those of controls with 46 (23.5%), 83 (42.3%) and 67 (34.21/6), respectively (p<0.0001). When the patents with schizophrenia were divided into the subgroups as disorganized, paranoid and residual. there was a significant difference In genotypic distribution among the subgroups (chi(2)=11.35, df=4 p=0.023). This association between -9Ala Mn-SOD allele and schizophirenia suggests that -9Ala variant may have a contribution in the physiopathogenesis of schizophrenia. Further investigations are warranted in larger populations with other susceptible genes that might be associated with schizophrenia. (C) 2004 Published by Elsevier Inc.
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    Levels of zinc and lipid peroxidation in acute coronary syndrome
    (Humana Press Inc, 2003) Cikim, G; Canatan, H; Gursu, MF; Gulcu, F; Baydas, G; Kilicoglu, AE
    The present study was carried out on 20 female patients diagnosed with acute coronary syndrome (ACS). The control group was composed of 20 healthy female volunteers. Plasma malondialdehyde (MDA) levels and serum zinc (Zn), total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, and VLDL-cholesterol, Lp(a), Apo-A(1), and Apo-B were determined in all patients and controls. Plasma MDA levels were determined to be significantly high in patients with ACS compared to the controls (1.75+/-0.27 vs 0.8+/-0.43 nmol/mL; p<0.05). On the other hand, Zn levels in patients with ACS were determined to be significantly low compared to the control group (67.9+/-14.8 vs 101.8+/-22.4 mg/dL; p<0.05). There was a statistically significant negative correlation between MDA and Zn levels in patients with ACS (r = -0.678, p<0.05). Other lipid parameters were significantly altered in patients with ACS compared to the controls (p<0.05). In conclusion, Zn and lipid peroxidation levels are important in patients with ACS and they must be monitored during diagnosis and treatment of these patients.
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    PCR/RFLP-based cost-effective identification of SOD2 signal (leader) sequence polymorphism (Ala-9Val) using NgoM IV: a detailed methodological approach
    (Elsevier, 2004) Akyol, O; Canatan, H; Yilmaz, HR; Yuce, H; Ozyurt, H; Sogut, S; Gulec, M
    Background: Superoxide dismutases (SOD) play an important role in the protection of cells and extracellular space from the products of oxidative stress. Two allelic variants have been described for the SOD2 gene (Ile58Thr involves a C to T substitution at nucleotide residue 339 and Ala-9Val involves a T to C substitution at nucleotide residue 1183). The enzyme proteins encoded by the different alleles have been suggested to have different activity patterns. Methods: The SOD2 polymorphism was determined using a polymerase chain reaction (PCR) and RFLP techniques with restriction endonuclease NgoM IV Results: The most available results were obtained from with 20 pmol primer final concentration in PCR reaction. A total of 20 pmol seems the cost-effective primer concentration with maximum quality. There were no difference between the band quality of 1-5 units of restriction endonucleases. On the other hand, short and long incubation times seem to be similar in order to obtain sharp bands on agarose gel. Conclusions: We have extended a method of SOD2 polymorphism (Ala-9Val) in mitochondrial sequence. This method provides the ability to genotype of SOD2, and it represents a fast, reliable, cost-effective and semi-automated methodology to determine SOD2 polymorphism in order to perform large-scale population studies. (C) 2004 Elsevier B.V. All rights reserved.