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Öğe The effect of thymus syriacus plant extract on the main physical and antibacterial activities of ZnO nanoparticles synthesized by SILAR method(Elsevier, 2022) Sahin, B.; Aydin, R.; Soylu, Soner; Turkmen, M.; Kara, M.; Akkaya, A.; Cetin, H.This study aimed to determine the main physical properties of green synthesized ZnO nanoparticles (ZnO-NPs) using Thymus syriacus plant extract and their antibacterial activities against eight different Gram-positive and Gram-negative plant bacterial disease agents. The surface morphological, structural, optical and electrical attributes of the ZnO films were analyzed by various types of characterization techniques. The authors have observed that the microstructural and surface morphological properties considerably changed by using T. syriacus plant extract. UV-Vis. analysis showed that the bandgap values are increased from 3.17 to 3.28 eV with the T. syriacus content in the growth solution. Impedance measurements presented an increase of the semicircle diameters with the increase % of T. syriacus. The antibacterial effectiveness of ZnO-NPs was evaluated against plant pathogenic bacterial species by using the agar disc diffusion method. Established on inhibition zone diameter values, ZnO-NPs displayed varying levels of antibacterial activities against all bacterial species tested. Gram-positive bacterial species were found more sensitive than Gram-negative species. In comparison, the highest antibacterial activity was displayed against Clavibacter michiganensis subsp. michiganensis and Bacillus subtilis subsp. subtilis, the lowest antibacterial activity was recorded for Pseudomonas corrugata. The present research findings suggest that the green synthesized ZnO-NPs have a prospective to be used as optoelectronic materials and antibacterial agents against plant pathogenic bacterial disease.Öğe A green approach for the preparation of nanostructured zinc oxide: Characterization and promising antibacterial behaviour(Elsevier Sci Ltd, 2021) Tasdemir, A.; Aydin, R.; Akkaya, A.; Akman, N.; Altinay, Y.; Cetin, H.; Sahin, B.In the present study, nanostructured zinc oxide (ZnO) films have been successfully synthesized using fruit extract of Viburnum opulus L. (VO) on glass slides by successive ionic layer adsorption and reaction (SILAR) procedure. The impact of VO concentrations on the structural, morphological, optical, electrical, and antibacterial attributes of ZnO films has been investigated in detail. The samples' XRD patterns present a hexagonal crystal structure with a preferential orientation along the (002) plane. The crystallite size values of ZnO samples were found to be in the ranges from 14.88 to 9.23 nm. The supplementation of VO to the synthesis solution remarkably affected the surface morphological features of the ZnO films. The optical results demonstrated that band gap energy values of the ZnO films at room temperature were decreased from 3.20 to 3.07 eV as a function of VO content in the bath solution. The films' electrical properties were determined by impedance analysis in the frequency range of 20 Hz -1 MHz. Impedance-frequency measurements showed VO insertion to ZnO thin films cause an increase in impedance value at the low frequencies. Cole-Cole plots with a single semi-circle confirmed the contribution of grain and grain boundary for the electrical conduction process. The agar disk diffusion method was used to test the antibacterial properties of ZnO/VO inserted ZnO and inhibition zones were measured. VO inserted ZnO showed a stronger inhibitory effect on gram-positive bacteria Staphylococcus aureus (ATCC 25923) and gramnegative bacteria Escherichia coli (ATCC 35218) than ampicillin antibiotic used as a control group. In line with the promising bactericidal results of a new generation, VO inserted ZnO, the nanostructured product with this study, it can also be applied in multidrug-resistant clinical isolates obtained from patients.Öğe Superior antibacterial activity against seed-borne plant bacterial disease agents and enhanced physical properties of novel green synthesized nanostructured ZnO using Thymbra spicata plant extract(Elsevier Sci Ltd, 2021) Sahin, B.; Soylu, Soner; Kara, M.; Turkmen, M.; Aydin, R.; Cetin, H.An easy and eco-friendly approach using Thymbra spicata var. spicata L. (TS) plant extract was developed for the formation of nanostructured ZnO. TS aqueous leaf extract was used for the green synthesis of nanostructured ZnO via the Successive ionic layer adsorption and reaction (SILAR) method. Electron microscope images exhibit the morphological adjustments of the samples with respect to change in TS concentration in the growth solution. The nanostructured ZnO grown by SILAR was observed to be polycrystalline with hexagonal crystal structure. The optical energy bandgap value of the samples varies from 3.21 to 3.09 eV as the content of TS increases from 2.5 to 5.0%. Also, the effect of TS additive to ZnO on electrical properties was investigated. It was determined by Van der Pauw measurements that TS contribution to ZnO significantly increased electrical resistance. In addi-tion, impedance analyzes of the produced films were carried out in the frequency range of 20Hz -1 MHz. Nyquist plots showed the single semicircle for all samples, and the values of capacitance and resistance were calculated. Its antibacterial activities was investigated against economically important Gram-positive (Clavibacter michiganensis subsp. Michiganensis) and negative (Pseudomonas syringae pv. Phaseolicola, Pseudomonas cichorii and Pectobacterium carotovorum subsp. Carotovorum) seed-borne plant bacterial disease agents by using paper disc diffusion assay for the first time. In vitro laboratory screenings of green synthesized nanostructured ZnO have given encouraging results, indicating their potential use in the management of seed-borne bacterial diseases.