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    AFLP analysis of genetic diversity in low chill requiring walnut (Juglans regia L.) genotypes from Hatay, Turkey
    (Elsevier Science Bv, 2007) Bayazit, S.; Kazan, K.; Gulbitti, S.; Cevik, V.; Ayanoglu, H.; Ergul, A.
    In this study, the genetic relatedness of 22 low chill requiring walnut genotypes adapted to the south east Mediterranean region of Turkey was analysed by amplified fragment length polymorphism (AFLP) markers. Relatively low level of genetic variation was detected among the genotypes examined by five AFLP primer combinations, suggesting that these walnut genotypes selected predominantly for their low chill requirement have relatively narrow genetic base. In addition, the geographical proximity of the genotypes analysed was not correlated with their level of genetic relatedness. These results have implications for walnut breeding and conservation. (c) 2006 Elsevier B.V All rights reserved.
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    High-resolution genetic analysis of the Sd-1 aphid resistance locus in Malus spp.
    (2002) Cevik, V.; King, G.J.
    Aphids cause serious physical and economic damage to most major crops throughout the world through feeding damage, with consequent symptom development and virus transmission. The rosy leaf-curling aphid (Dysaphis devecta Wlk.) is a pest of apple (Malus spp.) which displays an exceptionally clear phenotype with respect to susceptible and resistant symptoms. The Sd-1 locus for resistance to D. devecta biotypes 1 and 2 is present in Cox's Orange Pippin and its progeny and had previously been mapped to the top of linkage group 7. Detailed fine mapping of the locus was initiated with AFLP bulked segregant analysis of both pedigree and segregating bulks, which identified three new marker loci. Preliminary marker order in the Sd-1 region was established through mapping in a family derived from Prima x Fiesta, with additional segregation analysis on a Fiesta x Golden Delicious family. Previous recombinant data was re-evaluated and corrected. Two co-segregating AFLP fragments were found to contain a common (GA)23 repeat, from which a PCR-based simple sequence repeat (SSR) assay was developed. A high-resolution map around the Sd-1 region was established by analysing a large meta-population of Sd-1 recombinants using 759 additional individuals from different families. The Sd-1 gene has been located within a 1.3-cM interval flanked by the molecular markers SdSSRa and 2B12a and co-locates with the RFLP marker MC064. Allelism between Sd-1 and Sd-2 resistant sources was tested. Molecular markers tightly linked to Sd-1 were shown to be co-segregating with the Sd-2 locus, which indicated that Sd-1 and Sd-2 loci are at least tightly linked and, probably, allelic.