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    Diversity of halophilic archaea from six hypersaline environments in Turkey
    (Korean Soc Microbiology & Biotechnology, 2007) Ozcan, Birgul; Ozcengiz, Gulay; Coleri, Arzu; Cokmus, Cumhur
    The diversity of archaeal strains from six hypersaline environments in Turkey was analyzed by comparing their phenotypic characteristics and 16S rDNA sequences. Thirty-three isolates were characterized in terms of their phenotypic properties including morphological and biochemical characteristics, susceptibility to different antibiotics, and total lipid and plasmid contents, and finally compared by 16S rDNA gene sequences. The results showed that all isolates belong to the family Halobacteriaceae. Phylogenetic analyses using approximately 1,388 bp comparisions of 16S rDNA sequences demonstrated that all isolates clustered closely to species belonging to 9 genera, namely Halorubrum (8 isolates), Natrinema (5 isolates), Haloarcula (4 isolates), Natronococcus (4 isolates), Natrialba (4 isolates), Haloferax (3 isolates), Haloterrigena (3 isolates), Halalkalicoccus (1 isolate), and Halomicrobium (1 isolate). The results revealed a high diversity among the isolated halophilic strains and indicated that some of these strains constitute new taxa of extremely halophilic archaea.
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    Effective antibacterial and antioxidant properties of methanolic extract of Laurus nobilis seed oil
    (Triveni Enterprises, 2010) Ozcan, Birgul; Esen, Mari; Sangun, M. Kemal; Coleri, Arzu; Caliskan, Mahmut
    This study was carried out to determine the in vitro antimicrobial and antioxidant activities of the essential oil, seed oil, and methanolic extract of seed oil obtained from Laurus nobilis L. (Lauraceae). The methanolic extract of seed oil exhibited more effective antibacterial activity comparing to essential oil and seed oil. GC-MS analyses of the essential oil resulted in the identification of 25 compounds. 1.8-Cineol (44.72%), a-Terpinyl acetate (12.95%), Sabinene (12.82%) were the main components. The fatty acid composition was characterized with the high content of linoleic acid (40.79%) and lauric acid (38.08%). The 50% (IC50) inhibition activity of the essential oil on the free radical DPPH was determined as 94.655 mgml(-1), whereas IC50 value of methanolic extract of seed oil was found unstable. In the case of the linoleic acid system, oxidation of linoleic acid was inhibited by essential oil and methanolic extract of seed oil, which showed 64.28 and 88.76% inhibition, respectively The inhibition value of the methanolic extract of seed oil was quite close to the synthetic antioxidant BHT, 92.46% inhibition.
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    Öğe
    In vitro antimicrobial and antioxidant activities of various extracts of salvia microstegia (boiss.) et. bal. from antakya, Turkey
    (2009) Ozcan, Birgul; Esen, Mari; Coleri, Arzu; Yolcu, Hikmet; Caliskan, Mahmut
    The present study was performed to evaluate the in vitro antimicrobial and antioxidant properties of various extracts (hexane, dichloromethane, methanol/chloroform, methanol/water and direct methanol) from Salvia microstegia (Boiss.) Et. Bal., a member of Lamiaceae family. Direct methanol extracts of S. microstegia exhibited more effective antibacterial activities on the Gram-negative and Gram-positive bacteria. None of the extracts exhibited any antifungal activities on the Candida albicans ATCC 10231. The total phenolic components of S. microstegia was found 103.64 mg/g gallic acid equivalent. The antioxidant activity of the methanolic extract of S. microstegia was examined with two complementary test systems; 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ß-carotene-linoleic acid assays. The 50% (IC50) inhibition activity of the methanolic extract of S. microstegia on the free radical DPPH was determined as 7.63 mg/ml. In the case of the linoleic acid system, the methanolic extract of S. microstegia showed 79.92% inhibition, this value is quite close to the value of synthetic antioxidant reagent, 92.46%. © by PSP Volume 18-No 5a. 2009.
  • [ N/A ]
    Öğe
    IN VITRO ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF VARIOUS EXTRACTS OF Salvia microstegia (BOISS.) ET. BAL. FROM ANTAKYA, TURKEY
    (Parlar Scientific Publications (P S P), 2009) Ozcan, Birgul; Esen, Mari; Coleri, Arzu; Yolcu, Hikmet; Caliskan, Mahmut
    The present study was performed to evaluate the in vitro antimicrobial and antioxidant properties of various extracts (hexane, dichloromethane, methanol/chloroform, methanol/water and direct methanol) from Salvia microstegia (Boiss.) Et. Bal., a member of Lamiaceae family. Direct methanol extracts of S. microstegia exhibited more effective antibacterial activities on the Gram-negative and Gram-positive bacteria. None of the extracts exhibited any antifungal activities on the Candida albicans ATCC 10231. The total phenolic components of S. microstegia was found 103.64 mg/g gallic acid equivalent. The antioxidant activity of the methanolic extract of S. microstegia was examined with two complementary test systems; 2,2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene-linoleic acid assays. The 50% (IC50) inhibition activity of the methanolic extract of S. microstegia on the free radical DPPH was determined as 7.63 mg/ml. In the case of the linoleic acid system, the methanolic extract of S. microstegia showed 79.92% inhibition, this value is quite close to the value of synthetic antioxidant reagent, 92.46%.