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    Combination of cysteamine and lipoic acid improves the post-thawed bull sperm parameters
    (2016) Güngör, Şükrü; Aksoy, Adil; Yeni, Deniz; Avdatek, Fatih; Öztürk, Caner; Ataman, Mehmet Bozkurt; Coyan, Kenan; Bucak, Mustafa Numan; Başpınar, Nuri; Peker Akalın, Pınar
    The present study was conducted to examine the protective roles of cysteamine, trehalose, alpha-lipoic acid and combinations of these antioxidants on post-thawed bull sperm and oxidative stress parameters. Five healthy Holstein bull (3-4 years old) were used. Eight ejaculates for each bull were collected and pooled. Pooled ejaculate, splitted into seven equal aliquots and diluted at 37 °C with base extenders containing cysteamine 2 mM, trehalose 50 mM, alpha-lipoic acid (ALA) 1 mM, cysteamine 2 mM + trehalose 50 mM, ALA 1 mM + trehalose 50 mM, cysteamine 2 mM + ALA 1 mM and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The combination of cysteamine 2 mM and ALA 1 mM of the semen extender improved the percentages of post-thawed subjective motility (68 ± 2.7%), and progressive motility (42.9 ± 4.7%), compared with the controls (61 ± 4.2% and 37.5 ± 8%, respectively, non- significantly, P>0.05). The supplementation of the semen extender with combination of cysteamine 2 mM and ALA 1 mM produced a higher acrosome integrity and mitochondrial activity (52.02 ± 6.4% and 32 ± 4.1%, respectively), compared with the controls (30.5 ± 1.7 and 14.02 ± 3.5% respectively, P < 0.05). Combination of cysteamine and ALA antioxidants in semen extenders provided the benefit in terms of sperm motilities, acrosome integrity and mitochondrial activity on frozen-thawed bull sperm.
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    Influence of lycopene and cysteamine on sperm and oxidative stress parameters during liquid storage of ram semen at 5°C
    (Elsevier Science Bv, 2016) Peker Akalin, Pinar; Bucak, Mustafa Numan; Gungor, Sukru; Baspinar, Nuri; Coyan, Kenan; Dursun, Sukru; Ili, Pinar
    Ejaculates were collected from six Merino rams with the aid of an artificial vagina twice a week. The ejaculates containing spermatozoa with >80% forward progressive motility and concentrations higher than 2 x 10(6) spermatozoa/ml were pooled. The present study included two experiments. In experiment 1, each pooled ejaculate was divided into four equal aliquots and diluted (37 degrees C) with the Tris based extender, containing 0 (control), 0.5, 1 and 2 mM lycopene, at a final concentration of approximately 400 x 10(6) sperms/ml (single step dilution), In experiment 2, cysteamine at concentrations of 0 (control), 0.5,1 and 2 mM, was used as an additive in the extender, and the procedure explained above was applied for the division of aliquots and the dilution of semen. Diluted semen samples were kept in glass tubes and cooled from 37 degrees to 5 degrees C in a cold cabinet, and maintained at 5 degrees C. Sperm and oxidative stress parameters were evaluated after 0, 24, 48 and 72 h of storage at 5 degrees C. The extender supplemented with 0.5 mM lycopene resulted in higher mitochondrial activity rate (p<0.05) in comparison to the control group at 72 h of storage. Lycopene at 0.5 mM dose led to higher sperm motility rate (p<0.05) when compared to 2 mM lycopene group at 72 h of liquid storage. As regards oxidative stress parameters, only 2 mM lycopene increased total glutathione levels (p<0.05) at 0 h of storage. The extender supplemented with 1 mM cysteamine gave higher motility (p<0.05) at 48 h compared to control. As regards oxidative stress parameters, 1 and 2 mM cysteamine at 48 h and 1 mM cysteamine at 72 h increased total glutathione levels (p<0.05) compared to control groups. Cysteamine at 1 and 2 mM doses decreased lipid peroxidation (p<0.05) at 0 h of liquid storage compared to control. Our data suggest that lycopene at 0.5 and 2 mM and cysteamine at 1 and 2 mM doses can be added to Tris based extender for improving the ram sperm motility, viability, mitochondrial activity and oxidative stress parameters during the liquid storage. (C) 2016 Elsevier B.V. All rights reserved.
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    Relationship of blood and seminal plasma ceruloplasmin, copper, iron and cadmium concentrations with sperm quality in Merino rams
    (Elsevier Science Bv, 2015) Akalin, Pinar Peker; Bulbul, Bulent; Coyan, Kenan; Baspinar, Nuri; Kirbas, Mesut; Bucak, Mustafa Numan; Gungor, Sukru
    The aim of the current study was to investigate the concentrations of ceruloplasmin, copper, iron, zinc and cadmium concentrations in blood serum and seminal plasma obtained from Merino rams. In addition, their relationship with sperm parameters, fertility rate and litter size were also studied. Blood and ejaculate samples (6 replicates) were taken in October from 19 Merino rams, aged between 18 and 24 months. Ceruloplasmin, copper, iron, zinc and cadmium in blood serum and seminal plasma were determined. Sperm parameters including volume, mass motility, motility, concentration, Hos-test, viability, abnormal sperm and acrosome abnormality in semen, fertility rate and litter size were also evaluated. Highly positive correlation was found between blood ceruloplasmin and blood copper concentrations (r=0.812, p<0.001), whereas negative correlation were determined between these parameters in seminal plasma (r=0.195, p<0.05). Seminal plasma copper concentration was positively correlated with seminal plasma cadmium (r=0.206, p<0.05) and seminal plasma iron (r=0,305, p<0.01) concentrations. Negative correlation was determined between blood ceruloplasmin level and acrosomal defect (r=0.443, p<0.05). Seminal plasma ceruloplasmin level was positively correlated with volume (r=0.255, p<0.01) and negatively correlated with abnormal sperm (r=0.186, p=0.058) and acrosome abnormality (r=0.213, p<0.05). Seminal plasma iron concentration was positively correlated with other abnormality (r=0.257, p<0.01). Seminal plasma cadmium concentration was positively correlated with sperm abnormality (r=0.207,p=0.052) and other abnormality (r=0.262,p <0.05) and negatively correlated with fertility rate (r=0.449,p =0.054). Blood cadmium concentration was negatively correlated with litter size (r=0.579, 9<0.01). In conclusion, blood and seminal plasma ceruloplasmin may be suggested to have positive influence regardless of copper with its antioxidant property whereas iron and cadmium have negative influence on sperm parameters and fertility in Merino rams. (C) 2015 Elsevier B.V. All rights reserved.