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Öğe Chemical composition, antimicrobial and antibiofilm efficacy of C. limon and L. angustifolia EOs and of their mixtures against Staphylococcus epidermidis clinical strains(Elsevier Science Bv, 2018) Danila, Elena; Moldovan, Zenovia; Popa, Marcela; Chifiriuc, Mariana Carmen; Kaya, Alpaslan Durmus; Kaya, Madalina AlbuStaphylococcus epidermidis is a Gram-positive resident of the human skin, which in certain conditions, can turn into an opportunistic pathogen, being one of the most frequent agents of nosocomial infections, particularly of those associated with indwelling medical devices. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of two essential oils (EOs) to be used alone or in combination as natural alternatives to currently available antibiotics, for treating S. epidermidis infections or preventing the contamination and further development of S. epidermidis biofilm on viable tissues or medical devices. For this purpose C. Limon and L. angustifolia EOs were obtained both by hydrodistillation and microwave-assisted extraction and analyzed by GC MS. The compositions of EOs as a function of the extraction procedure were compared and discussed. Each of the two extraction techniques was more effective in the extraction of different components of the EOs. In the case of C. limon EOs, terpinen-4-ol, alpha-terpineol and linalool, not found in the microwave assisted extract, are obtained by classical hydrodistillation (being compounds of high polarity) while less polar compounds (alpha-pinene, beta-pinene, limonene, gamma-terpinene) are drawn into higher percentage through the microwave-assisted procedure. In the case of L. angustifolia EOs, a higher percentage of compounds with high polarity (such as linalool) is extracted by classical hydrodistillation while the compounds with lower polarity, such as acetates (linalyl acetate and neryl acetate) are extracted in higher proportion by the microwave-assisted extraction method. The antibacterial and antibiofilm effects of the EOs and of their mixtures were tested towards five S. epidermidis clinical strains and their biological activity was correlated with the oils composition. The most active composition was represented by the binary mixture (1:1) of the EOs obtained by hydrodistillation, containing terpene alcohols (such linalool) and D-limonene, which exhibited the lowest minimum inhibitory concentration (3.9 mu g/mL) and minimum biofilm eradicating concentration (0.97-7.81 mu g/mL), against all tested S. epidermidis strains. Other minor constituents, such as terpinen-4-ol, alpha-terpineol, linalyl acetate, neryl acetate, geranyl acetate and a-pinene could be potentiated in presence of limonene and could be responsible for the high antimicrobial activity of these EOs mixture.Öğe Comparative Study of Lavandula angustifolia Essential Oils Obtained by Microwave and Classical Hydrodistillation(Chiminform Data S A, 2018) Danila, Elena; Kaya, Durmus Alpaslan; Patrascu, Mariana; Kaya, Madalina Albu; Kumbakisaka, SylviuLavandula angustifolia which belong to Lamiaceae family with origins in the Mediterranean regionis one of the most important species of Lavandula. The most common method for obtaining the essential oil of lavender is described in the literature is steam hydrodistillation using a Neo-Clevenger, official method described in the European Pharmacopoeia. Another method described in the literature for obtaining oil of lavender is the extraction using microwave. The aim of this study was to obtain lavender essential oils bythese two different methods: classical hydrodistillation using Neo-Clevenger apparatus and microwave-assisted extraction. The yield of extraction was different for the two of methods 0.124 % for classical hydrodistillation and 0.6 % for microwave - assisted extraction. In order to establish and compare the chemical composition of lavender essential oils we used a gas chromatography coupled with mass spectrometry (GC-MS). In the classical hydrodistillation method 44 components with 99.20% yield and in microwave extraction, 46 components with 99.33% yields have been identified. The main compound of essential oil obtained was Linalool. But the other secondary main components are totally different. Even if the obtainedyield for lavender essential oil was bigger in the case of classical hydrodistillation, taking into account the time of extraction was lower for the oil obtained by microwave, it is recommended to continue with this modern method.Öğe COMPOSITE SCAFFOLDS FOR BONE REGENERATION MADE OF COLLAGEN/HYDROXYAPATITE/EUCALYPTUS ESSENTIAL OIL(Incdtp-Icpi, 2018) Florea, Andrei Dan; Danila, Elena; Constantinescu, Rodica Roxana; Kaya, Madalina Albu; Kaya, Alpaslan Durmus; Coara, Gheorghe; Albu, LuminitaBone regeneration is a serious problem nowadays because of the increased number of people suffering from infections, arthritis and bond loss. The aim of the present study was to develop and characterize collagen - hydroxyapatite - essential oil matrices for bone regeneration. In our study we wish to develop biomaterials which mimic bone composition and prevent allergic or toxic effects. The matrices obtained by freeze-drying were characterized by FT-IR analysis, water uptake capacity and microbiological analysis. The results obtained from analyses confirmed that collagen-hydroxyapatite-essential oils matrices exhibit proper characteristics for bone regeneration.Öğe Formulation and Characterization of Niacinamide and Collagen Emulsion and Its Investigation as a Potential Cosmeceutical Product(Mdpi, 2024) Danila, Elena; Kaya, Durmus Alpaslan; Anuta, Valentina; Popa, Lacramioara; Coman, Alina Elena; Chelaru, Ciprian; Constantinescu, Rodica RoxanaCosmeceuticals are one of the fast-growing areas of the natural personal care industry. Cosmeceuticals are cosmetic products with medicinal or drug-like benefits that can affect the biological functioning of the skin depending on the ingredients in the composition. The development of one formulation acting on the dermis and stimulating the collagen production is very important for the hydration of the skin. The association of collagen with other ingredients can have a positive effect on increasing the natural production of collagen in the skin. An example of such an ingredient is niacinamide, which, having a recognized nutritional value, has been quite recently studied. Considering these aspects, this study focused on developing oil-in-water (O/W) emulsions, based on natural ingredients (vegetable oils, floral waters, and essential oils) and hydrolyzed collagen and niacinamide as active ingredients, and on evaluating the stability, pH, optical, superficial, rheological and textural properties, as well as microbiological tests of the emulsions, in order to investigate their potential as a cosmeceutical product. All the obtained emulsions proved to be stable at variable temperatures and had a pH value compatible with natural pH of the skin, allowing their safe application. Over goniometric analysis, a partial wetting and a hydrophilic character of the emulsions were emphasized. Following the rheological analyses, all dermatocosmetic emulsions exhibited non-Newtonian pseudoplastic behavior and a thixotropic character, these properties being very important for their production process and their application on the skin surface to generate an optimal therapeutic effect. The textural characteristics recorded for all emulsions indicated adequate spreadability at the application site. All tested samples respected the Pharmacopoeia limits of microbiological contamination. All prepared emulsions have good stability, are safe for the skin and have appropriate physicochemical and microbiological characteristics; therefore, they can be used as a cosmeceutical product.Öğe OBTAINING AND CHARACTERIZATION OF SOME EMULSIONS BASED ON COLLAGEN HYDROLYSATE AND NATURAL EXTRACTS WITH A POTENTIAL ANTICELLULITIC ACTION(Polytechnic Univ Bucharest, 2019) Danila, Elena; Stan, Raluca; Enache, Adelina Elena; Turkmen, Musa; Kaya, Durmus Alpaslan; Kaya, Madalina Albu; Serafim, AndradaThe aim of this study was to formulate and evaluate O/W emulsions based on collagen and naturals ingredients such as vegetable oils and butters, caffeine, ivy extract and pink pepper essential oil. The essential oil of pink pepper was obtained by hydrodistilation and characterized by GC-MS. The main components of this essential oil are: alpha-Phelandrene 35.84%, Limonene 17.31%, alpha-Pinene 1.98% (monoterpenes) and beta-Phelandrene 13.04%, o-Cymene 4.65%, delta-Cadinene 3.27%, trans-Caryophyllene3.04%, Bicyclogermacrene 2.13% (sesquiterpenes). The emulsions obtained are stable and the pH values of emulsions correspond to the natural pH of the skin, indicating that emulsions can be safely applied to the skin. Results from the optical microscopy analysis show that emulsions have a creamy and foam like appearance. All the emulsions present adequate Theological properties. Further microbiological tests and other analysis are necessary for the obtained emulsions.Öğe Rheological Properties and Stability of Dermatocosmetic Emulsions with Collagen and Natural Ingredients Used as Color Correcting Cream and Cream Foundation(Revista Chimie Srl, 2019) Danila, Elena; Kaya, Durmus Alpaslan; Ghica, Mihaela Violeta; Kaya, Madalina Georgiana Albu; Negrea, Cristina; Popa, Lacramioara; Nitipir, CorneliaThe aim of this study was to formulate and evaluate two types of 0/W emulsions based on collagen and other natural ingredients that can be used as color correcting (CC-cream) and cream foundation. Both types of emulsions obtained are stable at different temperatures and the pH values of emulsions correspond to the natural pH of the skin, indicating that emulsions can be safely applied to the skin. Results from the optical microscopy analysis show that all emulsions present foam-like appearance. The rheological properties of emulsions were studied and it was proved that all emulsions present a non-Newtonian pseudoplastic behaviour and thixotropic characteristics at two operating temperatures (24 and 33 degrees C). It can be concluded that all emulsions can be used as a natural alternative to a CC-cream or a cream foundation. Further microbiological tests and clinical trials are necessary for the obtained emulsions.Öğe Rheological properties and stability of dermatocosmetic emulsions with collagen and natural ingredients used as color correcting cream and cream foundation(Syscom 18 SRL, 2019) Danila, Elena; Kaya, Durmus Alpaslan; Ghica, Mihaela Violeta; Kaya, Madalina Georgiana Albu; Negrea, Cristina; Popa, Lacramioara; Nitipir, CorneliaThe aim of this study was to formulate and evaluate two types of O/W emulsions based on collagen and other natural ingredients that can be used as color correcting (CC-cream) and cream foundation. Both types of emulsions obtained are stable at different temperatures and the pH values of emulsions correspond to the natural pH of the skin, indicating that emulsions can be safely applied to the skin. Results from the optical microscopy analysis show that all emulsions present foam-like appearance. The rheological properties of emulsions were studied and it was proved that all emulsions present a non-Newtonian pseudoplastic behaviour and thixotropic characteristics at two operating temperatures (24 and 33°C). It can be concluded that all emulsions can be used as a natural alternative to a CC-cream or a cream foundation. Further microbiological tests and clinical trials are necessary for the obtained emulsions. © 2019 SYSCOM 18 S.R.L.. All rights reserved.Öğe Selection of Optimal Operating Conditions for Extraction of Myrtus Communis L. Essential Oil by the Steam Distillation Method(Mdpi, 2020) Kaya, Durmus Alpaslan; Ghica, Mihaela Violeta; Danila, Elena; Ozturk, Sevket; Turkmen, Musa; Albu Kaya, Madalina Georgiana; Dinu-Pirvu, Cristina-ElenaMyrtus communis L. is one of the important aromatic and medicinal species from the Mediterranean area. It is used in various fields such as culinary, cosmetic, pharmaceutical, therapeutic, and industrial applications. Thus, a Box-Wilson experimental plan was used in this study to select the optimal operating conditions in order to obtain high volumes of essential oils. The factorial design method was applied to evaluate at an industrial scale the effect of major process variables on the essential oil extraction from Myrtus communis L. herbs by the steam distillation method. The input variables considered as significant operating conditions were: X-1-boiler occupancy rate (boilers were filled to 50%, 75%, and 100%), X-2-distillation duration (distillation was continued 60, 75, and 90 min), and X-3-particle size (herbs were cut in sizes of 10, 20, and 30 mm via guillotine). The dependent variable selected, coded as Y, was the essential oil volume obtained (mL). The steps of the classical statistical experimental design technique were complemented with the Taguchi method to improve the extraction efficacy of essential oil from Myrtus communis L., and the optimum parameter conditions were selected: boiler occupancy rate 100%, distillation duration 75 min, and particle size 20 mm. Following the optimum parameters, the GC-MS assay revealed for the Myrtus communis L. essential oil two predominant components, alpha -pinene-33.14% and eucalyptol-55.09%.
