Yazar "Disli, H. Burak" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim
    Öğe
    Escherichia coli O157 in fish: Prevalence, antimicrobial resistance, biofilm formation capacity, and molecular characterization
    (Elsevier, 2020) Onmaz, Nurhan Ertas; Yildirim, Yeliz; Karadal, Fulden; Hizlisoy, Harun; Al, Serhat; Gungor, Candan; Disli, H. Burak
    This study was performed to survey the incidence of Escherichia coli O157:H7 contamination in fish samples which were obtained from different fish farms and retail markets. For this purpose, a total of 140 fish samples were analyzed according to ISO 16654 and screened for virulence genes by mPCR. Antibiotic susceptibility tests were performed with the disc diffusion method and isolates were genotyped by using Enterobacterial repetitive intergenic consensus (ERIC) PCR. Of the 140 analyzed sample, two (1.4%), from the same farm, were found to be contaminated with E. coli O157 serogroup, one of which harbored stx1 and the other eaeA gene. E. coli O157 serogroup were resistant to only ciprofloxacin and were not capable of forming biofilm and their ERIC-PCR patterns were different. In conclusion, the existence of pathogenic E. coli O157 serogroup in fish samples might be a significant threat to public health and fish could serve as a vehicle for transmission of these bacteria to consumers in Turkey.
  • Küçük Resim
    Öğe
    From cattle to pastirma: Contamination source of methicillin susceptible and resistant Staphylococcus aureus (MRSA) along the pastirma production chain
    (Elsevier, 2021) Gungor, Candan; Barel, Mukaddes; Dishan, Adalet; Disli, H. Burak; Koskeroglu, Kursat; Onmaz, Nurhan Ertas
    This study was designed to determine the prevalence of Methicillin Susceptible S. aureus (MSSA) and MethicillinResistant S. aureus (MRSA) and their source of contamination in the pastirma production chain. Additionally, this study was focused on the antimicrobial resistance, virulence profiles, biofilm-forming capabilities and phylogenetic relationships of obtained isolates. A total of 400 samples were analyzed and from which 105 (26.25%) were found positive for coagulase-positive with Staphylococci (CPS). Within the 105 CPS samples, 36 (9%) were identified as S. aureus, from which 8 (2%) were MRSA. Four (11.1%) of 36 S. aureus isolates, of which 3 (37.5%) were MRSA, had a multidrug resistance (MDR), and 6 MRSA strains were found positive for one or more SEs genes (seb, sed, and see). According to the ERIC-PCR analysis, only two S. aureus strains (one with personnel origin and one with carcass origin) were genetically identical. This study highlights the detection frequency of S. aureus in samples analyzed observed, while low, can be a significant public health problem, especially due to the identification of MRSA harboring some enterotoxin genes and having MDR.
  • Küçük Resim
    Öğe
    Profiles of Campylobacter jejuni from raw retail chicken meat: genetic diversity, pathogenic features, and antibiotic resistance
    (Wiley, 2024) Hizlisoy, Harun; Barel, Mukaddes; Dishan, Adalet; Al, Serhat; Gungor, Candan; Koskeroglu, Kursat; Disli, H. Burak
    The study aimed to assess Campylobacter jejuni prevalence in chicken meat, biofilm formation, virulence factors, antibiotic resistance, and molecular typing. In the study, 200 chicken meat samples were collected from local outlets and 51 (25.5%) isolates were identified as C. jejuni. Resistance rates to ampicillin, tetracycline, sulfamethoxazole/trimethoprim, and ciprofloxacin were 59%, 60%, 64%, and 64% respectively. Many of the isolates (49%) exhibited multidrug resistance. Beta-lactamase and tetracycline resistance genes were found in 82.3% and 86.2% of isolates, respectively. Virulence genes were detected in various proportions. Biofilm formation was weak to moderate. ERIC-PCR showed varied band profiles. Whole genome sequencing confirmed findings. The study highlights C. jejuni presence with antibiotic resistance, virulence and biofilm features in chicken meat, posing public health risks.