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Öğe Anti-Müllerian Hormone Expression in the Ovarian Follicles and Factors Related to Serum Anti-Müllerian Hormone Concentrations in the Domestic Queens(Hellenic Veterinary Medical Soc, 2024) Gozer, A.; Dogruer, G.; Gokcek, I; Kutlu, T.; Bahan, O.; Yagci, I. PirAnti-M & uuml;llerian Hormone (AMH) is a glycoprotein with 140 kDa molecular weight secreted by the granulosa cells, playing a crucial role in folliculogenesis. The present study aimed to reveal (1) the relationship between age, body weight, estrouscycle, and ovariohysterectomy with the serum AMH concentrations and (2) expression of the AMH in the ovarian follicles. Serum AMH concentrations were determined by a commercial ELISA. Ovarian expression of the AMH in the granulosa cells was detected by immunohistochemistry. Twenty femalecats were allocated to three groups according to the period of the sexual cycle, age, and body weight. The phaseof their estrouscycle was determined according to serum estradiol 17 beta and progesterone levels. Blood samples were collected to detect serum AMH concentration on Day 0- preoperative, Day 3, and Day 10 -postoperative. There was a decrease in AMH concentration on Day 3 compared to the preoperative level (Day 0) (P<0.001).No statistical difference in AMH concentration wasfound between Day 3 and 10 postoperative (P>0.05). Furthermore, the period of the sexual cycle, age, and body weight did not affect AMH levels (P>0.05). In immunohistochemical analyses,a mild AMH expression was first observed in the granulosa cells of the primary follicles, and moderate AMH expression was observed in the granulosa cells of the secondary follicles. Strong AMH expression was observed in the cumulus cells and mild AMH expression in the mural granulosa cells of the antral follicles. In conclusion, ovariohysterectomy caused a definite decline in serum AMH concentration, and AMH expression was found in all the ovarian follicles except for primordial follicles in domestic cats.Öğe Estimation of periostin as a biomarker for early pregnancy diagnosis in goats: a preliminary study(Shiraz Univ, 2021) Kose, A. M.; Saribay, M. K.; Urer, Koldas E.; Naseer, Z.; Dogruer, G.; Karaca, F.; Cetin, Coskun N.Background: Periostin (POSTN) is an extracellular matrix (ECM) protein that plays an important role in the metastatic process and cancer cell migration. As implantation is a similar mechanism to metastasis, it has been hypothesized that POSTN may also play a role in the implantation process. Aims: The aim of the present study was to compare POSTN and progesterone levels during the early pregnancy stage in Damascus goats. Methods: Forty goats were synchronized using progesterone based sponges and were mated upon estrus signs display. While ten goats were kept as control (CON) and were not allowed to mate. Blood samples were taken through jugular venepuncture from CON and synchronized goats on day 13, 15, 17, 19, and 21 of breeding. Progesterone and POSTN levels were determined by enzyme-linked immunosorbent assay (ELISA). Later the pregnancy diagnosis was confirmed by transabdominal ultrasonography on day 50 after mating. Results: Progesterone level was influenced by status of pregnancy and day of observation with an interaction between the status of pregnancy and day of observation in goats. Whereas POSTN level was only affected by the day of observation. Conclusion: POSTN level did not vary with progesterone level during phase of embryonic implantation in goats; however, standardization and application of different procedures for POSTN assay in a large group of animals might be useful as an early pregnancy biomarker in goats.Öğe Estrus synchronization during transition period, timed artificial insemination (TAI) and the effect of GnRH administration at the TAI on fertility in lactating goats(Wiley-Blackwell, 2011) Saribay, M. K.; Karaca, F.; Dogruer, G.; Ates, C.[Abstract Not Available]Öğe Glucose-6-phosphate dehydrogenase, glutathione peroxidase, total glutatione and reduced nicotinamide adenine dinucleotide phosphate in milk cells of subclinical mastitic cows(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2019) Akalin, P. P.; Ergun, Y.; Baspinar, N.; Dogruer, G.; Kucukgul, A.; Cantekin, Z.; Isgor, M.This study aimed to determine the levels of milk cell total protein (TP), reduced nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), activities of glucose-6-phosphate dehydrogenase (G6PD) and glutathione peroxidase (GPx) in subclinical mastitic cows. Milk from each udder was collected and grouped by the California Mastitis Test. Then, a somatic cell count (SCC) was performed, and the groups were re-scored as control (5-87 x 10(3) cells), 1st group (154-381 x 10(3) cells), 2nd group (418-851 x 10(3) cells), 3rd group (914-1958 x 10(3) cells), and 4th group (2275-8528 x 10(3) cells). Milk cell TP, NADPH, tGSH levels, G6PD, and GPx activities were assessed. Microbiological diagnosis and aerobic mesophyle general organism (AMG, cfu/g) were also conducted. In mastitic milk, TP, NADPH, and tGSH levels, and G6PD and GPx activities were significantly reduced per cell (in samples of 10(6) cells). In addition, milk SCC was positively correlated with AMG (r=0.561, p<0.001), NADPH (r=0.380, p<0.01), TP (r=0.347, p<0.01) and G6PD (r=0.540, p<0.001). There was also positive correlation between NADPH (r=0.428, p<0.01), TP (r=0.638, p<0.001) and AMG. NADPH was positively correlated with TP (r=0.239, p<0.05), GPx (r=0.265, p<0.05) and G6PD (r=0.248, p=0.056). Total protein was positively correlated with tGSH (r=0.354, p<0.01) and G6PD (r=0.643, p<0.001). There was a negative correlation between tGSH and GPx activity (r=-0.306, p<0.05). The microbiological analysis showed the following ratio of pathogens: Coagulase-Negative Staphylococci 66.6%, Streptococcus spp 9.5%, Bacillus spp 9.5%, yeast 4.8%, and mixed infections 9.5%. As a conclusion, when evaluating the enzyme and oxidative stress parameters in milk, it is more suitable to assign values based on cell count rather than ml of milk. The linear correlation between the SCC and AMG, milk cell NADPH, TP and G6PD suggests that these parameters could be used as markers of mastitis.Öğe Variations in serum metabolites in response to season, cyclicity, and pregnancy in estrus-synchronized Damascus goats(Springer, 2020) Saribay, M. K.; Naseer, Z.; Dogruer, G.; Ozsoy, B.; Ates, C. T.The current study was carried out to determine the changes in serum electrolytes, liver enzymes, and hormones during breeding and non-breeding seasons in estrus-synchronized goats. Forty goats (n = 40) were synchronized by inserting the sponges intravaginally for 12 days; PMSG and PGF(2)alpha were injected on day of sponge removal. Blood samples were collected from the goats at the time of sponges insertion (day -12), estrus day (day 0), and at 15 day after sponge withdrawal (day 15). The blood samples were analyzed for glucose, cholesterol (CHO), aspartate aminotransferase (AST), alanine aminotransferase (ALT), thyroid stimulating hormone (TSH), triiodothyronine (T-3), thyroxine (T-4) and progesterone (P-4), calcium (Ca), phosphate (PO4), magnesium (Mg), sodium (Na), potassium (K), and chlorine (Cl) levels. Repeated measurements ANOVA of general linear model were used as statistical model. The results showed that seasons have an effect (p < 0.05) on glucose, Ca, Na, K, Cl, CHO, T-3, and T-4 levels. There was an effect (p < 0.05) of cyclicity only on P-4 level. Whereas, nonpregnant, or goats carrying singlet and twin fetuses have shown variation (p < 0.05) in glucose, Ca, and P-4 levels. Although, the correlations existed between different serum metabolites but strong correlations (p < 0.05) were observed either between Ca and Na or T-3 and T-4. In conclusion, the serum electrolytes and liver enzymes are correlated with hormones during the different seasons, pregnancy status, and fecundity. In recommendation, the results of the study could be as managemental tool to monitor the reproductive activity across seasons and to maintain pregnancy carrying twining in goat breeds.
