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    Concentration of essential and non-essential elements and carcinogenic/non-carcinogenic health risk assessment of commercial bee pollens from Turkey
    (Elsevier Gmbh, 2023) Sevin, Sedat; Tutun, Hidayet; Yipel, Mustafa; Aluc, Yasar; Ekici, Husamettin
    Background: Bee pollen, known as a natural super-food with valuable nutritional ingredients, is regarded as a good indicator of ecotoxic substances, such as potentially toxic elements (PTEs). Therefore, this study aims to examine the concentrations of selected PTEs (Al, As, Ba, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Mo, Ni, Pb, Se, Sn, Sr, V, Zn) in bee pollen purchased from online markets in Turkey and perform a health risk assessment to identify the potential risk to consumers.Methods: The quantitative analyses were conducted by inductively coupled plasma optical emission spectrometry (ICP-OES).Results: The mean values of essential PTEs in decreasing content order were Mg > Fe > Zn > Mn > Cu > Ni > Se > Cr > Mo >Co = V. Regarding the results of the study, daily consumption (40 g for adult or 20 g for children) of commercial bee pollen can recompense 20-35 % of daily Cu, Mn, Se requirements for children, adults, pregnant, and breastfeeding women. The decreasing content order of non-essential elements was Al > Sn > Sr > Ba > Pb > As. Cadmium and Hg concentrations were below the detection limits in all the samples. In terms of food and public health; detection of the PTEs concentrations is necessary to assess the quality and safety of bee pollen before consumption. According to the carcinogenic and non-carcinogenic risk assessments; commercial pollen consumption does not pose a health risk to either children or adults for the PTEs monitored in this study.Conclusion: We conclude that bee pollen is an ideal indicator for the monitoring of environmental pollution of PTEs and also a valuable source of essential elements. This study highlights the need to develop standards that regulate acceptable concentrations of PTEs.
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    Concentrations of Essential and Non-essential Toxic Trace Elements in Wild Boar (Sus Scrofa L., 1758) Tissues from Southern Turkey
    (Springer, 2014) Yarsan, Ender; Yipel, Mustafa; Dikmen, Bilal; Altintas, Levent; Ekici, Husamettin; Koksal, Ali
    Wild animals, including wild boars, are suitable for use as bioindicators. The aim of this study was to evaluate the concentrations of selected essential (Co, Cu, Fe, Se, Zn) and non-essential (As, Cd, Pb) potentially toxic trace elements in various tissues (hair and hoof) of wild boars hunted in the vicinity of Antalya province in Turkey, in relation to hunting seasons. Concentrations in mg/kg on dry weight basis were determined as 0.37 +/- A 0.27 mg/kg for As, 0.05 +/- A 0.04 mg/kg for Cd, 0.24 +/- A 0.33 mg/kg for Co, 4.84 +/- A 2.48 mg/kg for Cu, 289.94 +/- A 165.26 mg/kg for Fe, 8.71 +/- A 15.68 mg/kg for Pb, 0.24 +/- A 0.10 mg/kg for Se, and 28.99 +/- A 21.41 mg/kg for Zn in the hair samples; and as 0.11 +/- A 0.04 mg/kg for As, 0.01 +/- A 0.04 mg/kg for Cd, 0.01 +/- A 0.01 mg/kg for Co, 1.03 +/- A 0.25 mg/kg for Cu, 56.88 +/- A 18.68 mg/kg for Fe, 0.30 +/- A 0.18 mg/kg for Pb, 0.11 +/- A 0.05 mg/kg for Se, and 17.91 +/- A 10.98 mg/kg for Zn in the hoof samples.
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    The content and health risk assessment of selected elements in bee pollen and propolis from Turkey
    (Academic Press Inc Elsevier Science, 2022) Tutun, Hidayet; Aluc, Yasar; Kahraman, Hatice Ahu; Sevin, Sedat; Yipel, Mustafa; Ekici, Husamettin
    This study aimed to determine the concentrations of potentially toxic elements (PTEs), including Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, Sn, V, and Zn, in pollen and propolis samples collected from migratory beekeeping areas in Turkey. A health risk assessment was performed to identify the potential risk of these PTEs to consumers in terms of public health. Concentrations of the elements were analyzed by inductively coupled plasma optical emission spectroscopy. The most abundant element in the bee pollen and propolis samples was Fe, with average concentrations of 47.3 and 390 mg kg(-1), respectively, followed by Al, Zn, Mn and Sn. Concentrations of all elements except Cu, Mn and Sn were higher in propolis than in pollen. The concentrations of Fe, Al, and Zn in the propolis samples were over eight, seven, and two times higher than in the bee pollen samples, respectively. Se, Cd, and Hg in pollen and Se and Cd in propolis were below the detection limits. Pb, Co, and Cr were detected below 0.62, 0.06, and 0.91 mg kg(-1), in pollen samples, respectively. Co and Hg were detected below 0.68, and 0.18 mg kg(-1), in propolis samples, respectively. Detection of the PTEs contamination level and assessment of the health risks in pollen and propolis is necessary to ascertain quality and safety before consumption. In this study, we concluded that bee pollen and propolis may be good indicators for the screening of environmental pollution with PTEs and standards regulating acceptable concentrations of these pollutants in bee products should be established. In addition, it has been determined that consumption at the specified rates does not pose a risk. This study suggests the determination of admissible concentrations of PTEs in bee pollen and propolis.
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    Contractile Effects of Eryngium kotschyi Boiss. on Rat Isolated Ileum and Detrussor Muscle
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2014) Baydan, Emine; Kartal, Murat; Yurdakok, Begum; Erdem, Sinem Aslan; Ince, Sinan; Ekici, Husamettin; Alp, Harun
    The pharmacological activity of the aerial (EKA) and root (EKR) parts of the endemic plant, Eryngium kotschyi Boiss., on rat isolated ileum and detrusor muscle was investigated. Plant extracts alone and with the presence of agonist (acethylcholine) and antagonist (atropin, verapamile, oxybutinine-detrusor muscle, and papaverine-ileum) drugs, along with Ca2+ applications on calcium-free medium, were applied. Plant extracts induced contraction in ileum and detrusor muscle where the contractions were concentration dependant for EKA and EKR single dose applications in detrusor muscle and concentration-free contractions were observed in cumulative applications for both tissues. Aerial and root parts of Eryngium extracts induced contractions in dose, tissue and protocol dependent manner where the contractions were affected by the tested antagonists, which could be attributed to non-specific pathways including calcium ions and calcium channel stimulations.