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    Interpretation of the morphological characteristics of Sehir creek basin (Ispir) regarding fluvial geomorphology and regional tectonics
    (Elsevier Science Bv, 2014) Karatas, Atilla; Ekinci, Deniz
    Investigations on the morphometric characters of basins facilitate our understanding regarding many issues about the structure of the earth's crust, the life of earth and the related factors and processes. Those quantity-based studies that ensure provision of more concrete data about the fluvial process in the basins that are shaped by the rivers make it possible to generate more consistent and well pointed interpretations. In this context, some morphometric characteristics were identified in Sehir Creek Basin. The issues pointed by the data obtained in this manner were assessed to analyze and interpret basin morphology. Accordingly; various factors that affect the shaping of the basin and their correlations were identified and it was determined that the basin is between youth and maturity stages in terms of erosion cycle however it also includes sections that reflect complete youth stages based on neo-tectonic activities seen in some parts. A serious asymmetry was observed between the source and estuary sections of the basin that shows a relation with the young faults that were active in the Quaternary which were not included in any geology maps of the area. Results obtained by interpreting the morphometric analyses were investigated and confirmed by field studies as well. (C) 2013 The Authors. Published by Elsevier Ltd.
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    Purification and characterization of nitric oxide synthase from bovine kidney and investigating drug-induced toxicities of some antibiotics on the enzyme activity
    (Bentham Science Publishers B.V., 2017) Işgör, Mehmet Mustafa; Ekinci, Deniz; Beydemir, Şükrü
    Introduction: Broad spectrums of antibiotics are widely used in the treatment of bacterial infections, particularly causing Staphylococcus aureus. These drugs have high acute renal injury (AKI) potency due to an increase in nitric oxide level. In this study, potency of nitric oxide synthase inhibition of some antibiotics was investigated according to vancomycin which was used as a reference antibiotic in renal injury. Methods and Results: Nitric oxide synthase (NOS) enzyme was purified by using 2'5'-ADP Sepharose 4B affinity chromatography from bovine kidney tissue in a single step with a yield of 3.58% and 1217-fold. Native and subunit molecular weights of the purified kidney NOS enzyme were calculated as 215 kDa and 114.8 kDa. Optimum pH, optimum ionic strength, optimum temperature and stable pH values for purified enzyme were determined as 7.5, 50 mM, 10°C and a pH range of 5.5-6.5, respectively. In vitro effects of antibiotics on purified enzyme activity were investigated by drawing Lineweaver-Burk plots. Enrofloxacin, kanamycin, chloramphenicol, gentamicin, vancomycin, cefazolin, streptomycin and ampicillin inhibited enzyme activity. IC50 values of these compounds were determined as 0.189 mM, 0.295 mM, 1.509 mM, 6.614 mM, 16.579 mM, 18.679 mM, 28.171 mM and 30.394 mM, respectively. Conclusion: Enrofloxacin and kanamycin were observed to have stronger NOS inhibitory effects as compared to vancomycin and may be more reliable antibiotics for use in renal infections as an alternative to vancomycin. © 2017 Bentham Science Publishers.