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    Anticandidal Activity and Anticandidal Mechanism of Essential Oil of Cuminum cyminum L. and Myrtus communis L. Mixture
    (Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Eliuz, Elif Ayse Erdogan; Bahadirli, Nadire Pelin
    C. cyminum (cumin) and M. communis (myrtle) comprise many biologically active molecules. In this study, cumin seed and myrtle leaves were mixed then hydrodistilled and analyzed by GC-MS. The main components of the essential oil were cuminal (50.71%), 1,8 cineole (8.30%), O-cymene (7.88%), beta-pinene (7.62%), a-pinene (7.16%),.-terpinene (6.09%) and a-terpinolene (2.19%). The antifungal activity of the essential oil against C. albicans, C. parapsilosis and C. tropicalis was investigated using spectrophotometric broth microdilution, colorimetric broth microdilution, and agar well diffusion tests. Antimicrobial mechanism of the EO was researched by TTC-dehydrogenase relative activity, protein and DNA leakage analysis. While the MIC values of the oil were 3.29 mg mL(-1) for C. albicans, 3.57 mg mL(-1) for C. parapsilosis and 3.65 mg mL(-1) for C. tropicalis, the 50% cytotoxic concentration values were between 0.17 mg mL(-1) and 2.61 mg mL(-1) for Candida species. The inhibition values against yeasts in dark and light conditions were found as 42.1 mm on C. parapsilosis and 39 mm on C. tropicalis exposed dark and light, respectively. The inhibition zones of oil in water, glucose, glycerol and salt environment. As a result, the highest IZ was found as 26.6 mm on C. albicans and 26 mm on C. tropicalis in the presence of a 3% glycerol environment. The DNA and protein levels were increased when yeast was exposed to the EO. As a result, the oil of mixed C. cyminum and M. communis preserved its antimicrobial stability in different environmental conditions and should contribute to new antifungal research.