Yazar "Elmali, Mehmet" seçeneğine göre listele
Listeleniyor 1 - 12 / 12
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Antimicrobial susceptibility and virulence-associated genes in Campylobacter isolates from milk and wastewater in Hatay, Turkey(Univ Federal Santa Maria, 2019) Elmali, Mehmet; Can, Hayriye YesimCampylobacter is one of the most common causes of bacterial foodborne diseases throughout the world This study was conducted to determine the prevalence, antimicrobial resistance and virulence of Campylobacter isolates of raw cow's milk and cattle slaughterhouse wastewater samples in Hatay, Turkey. A total of 114 raw milk and 78 wastewater samples were analyzed for the identification of C. jejuni, C. coli, and C. lari by multiplex PCR. The overall prevalence of Campylobacter was found to be 7.2%. of these isolates, 85.7% were identified as C. jejuni and 14.2% as C. coli, but C. lari was not detected in the study. The cdtA and cadF genes were present in 66.6% and 41.6% of C. jejuni isolates tested, respectively, but wlaN gene was not found in any of the isolates. Results of antimicrobial resistance analysis showed that 71.4% of the isolates were resistant to erythromycin, 64.2% to tetracycline, and 57.1% to ciprofioxacin. Overall, 8 of 14 Campylobacter isolates (57.1%) showed a multidrug resistance.Öğe Carcass and Meat Quality Characteristics of White and Multicolor Geese under Local Breeder Conditions(Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Yakan, Akin; Elmali, Dilek Aksu; Elmali, Mehmet; Sahin, Tarkan; Motor, Sedat; Can, YesimThe purpose of this study was to define the meat quality characteristics of White and Multicolor geese under local breeder conditions. While carcass and edible offal percentages were similar breast, back and wing ratios were statistically different between White and Multicolor geese. Phenotypic color differentiation between geese varieties was reflected on both breast meat and skin (except a* value). While White geese had better values than Multicolor geese for saturated fatty acid and monounsaturated fatty acid, Multicolor geese were higher than White geese for polyunsaturated fatty acid. Nutritive value of White variety was higher than Multicolor variety in breast meat, skin and abdominal fat.Öğe Detection of Coxiella burnetii in cows', goats', and ewes' bulk milk samples using polymerase chain reaction (PCR)(Croatian Dairy Union, 2015) Can, Hayriye Yesim; Elmali, Mehmet; Karagoz, AlperQ fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. This study was planned to determine the prevalence of C. burnetii in cows', goats', and ewes' bulk milk (BM) samples using PCR and to confirm positive results by DNA sequencing. A total of 150 BM samples (50 samples of each cows', goats', and ewes' milk) collected from 15 randomly selected dairy farms in Hatay province were analyzed. The BM samples were taken between January 2012 and July 2013. Bacterial DNA was extracted directly from milk samples. Nine of the BM samples (6 %) were PCR positive; five from cows' BM, two from ewes' BM, and two from goats' BM. Bacterial DNA was detected in 3 of 15 (20 %) dairy farms. Positive results were confirmed by DNA sequencing. The sequencing results of Coxiella DNA extracted from cows', goats', and ewes' milk samples were consistent with the reference strain with 100 %, 99.7 %, 99.8 % homology, respectively. BM samples were found to be contaminated with C. burnetii; therefore, taking hygienic measures is necessary for food safety and public health.Öğe Detection of Listeria monocytogenes by using PCR method in refrigerated ready-to-eat food consumed in Turkey(Polish Soc Veterinary Sciences Editorial Office, 2013) Elmali, Mehmet; Oner, Suleyman; Yaman, HilmiThis study aimed to detect the prevalence, risk and importance of Listeria monocytogenes in ready-to-eat (RTE), traditional food products, using PCR in the Antakya/Hatay district in the southeast of Turkey, which is a popular tourist destination. L. monocytogenes was isolated and identified from 2.09% of the 239 RTE food samples tested. L. monocytogenes was found in 3 (2.58%) of 116 salad samples, and 2 (6.66%) of 30 abagannus samples. However, no L. monocytogenes contamination was detected in the samples of broad bean paste, hummus (crushed chickpeas), parsley salads, traditional salted yoghurt, thyme salads, and walnuts with red pepper. In conclusion, our study showed a relatively high risk of L. monocytogenes in some traditional RTE foods in the Antakya/Hatay district of Turkey. The observed L. monocytogenes contamination may have been caused by a high microbial load of the ingredients, cross-contamination, poor production and processing conditions, incorrect handling, or watering vegetables with polluted water.Öğe Detection of Salmonella spp., Salmonella Enteritidis, Salmonella Typhi and Salmonella Typhimurium in cream cakes by polymerase chain reaction (PCR)(Polish Soc Veterinary Sciences Editorial Office, 2014) Can, Hayriye Yesim; Elmali, Mehmet; Karagoz, Alper; Oner, SuleymanThe aims of this study were: (i) to determine the incidence of Salmonella spp. by conventional culture methods in cream cakes, (ii) to detect invA gene by PCR for the confirmation of the isolates, (iii) to analyze isolates for S. Enteritidis, S. Typhi, and S. Typhimurium by PCR based on Sdf I, ViaB, Spy gene sequences, respectively, and (iv) to identify isolates in comparison to the reference strain by DNA sequence analysis. A total of 81 unpackaged cream cake samples were obtained from different patisseries in Hatay, Turkey. Salmonella spp. was detected in 13 (16%) out of 81 cream cake samples by the conventional culture method. A total of 45 isolates from the 13 positive samples were confirmed as Salmonella spp. by PCR. Homology among the reference strain and isolates and homology within the isolates was found to be 98.97-100%. Cream cake samples analyzed in this study were found to be contaminated with Salmonella, thus posing a potential health hazard for the consumer. To protect public health, food safety management systems such as HACCP, GMP and GHP could be carried out in cream cake production.Öğe Inhibitory effects of different decontamination agents on the levels of Listeria monocytogenes in the experimentally inoculated raw beef samples in the laboratory conditions(Veteriner Fakultesi Dergisi, 2012) Elmali, Mehmet; Yaman, Hilmi; TekInşen, Kemal Kaan; Öner, Süleyman; ÇekIn, ErhanThis study aimed at comparing the inhibitory effects of various decontamination agents (1% lactic acid, 2% lactic acid, 2% acetic acid, 0.1% acidified sodium chloride, 0.1% sodium acetate, and 0.1% cetylpridinium chlorine) on experimentally contaminated raw beef samples with Listeria monocytogenes being a significant pathogen microorganism for public health. The highest level of bacterial inhibition was determined in the meat samples treated with 2% lactic acid while the lowest level was in the samples treated with 0.1% acidified sodium chloride. It is very important to specify that initial bacterial load of carcases, the decontamination technique applied, and the characteristics of acids used (dissociated or undissociated, pH, amount, percentage, application temperature, and different combinations) are essential parameters to be taken into account in the process of reducing microorganism on raw meat samples.Öğe Molecular Typing and Antimicrobial Susceptibility of Staphylococcus aureus Strains Isolated from Raw Milk, Cheese, Minced Meat, and Chicken Meat Samples(Korean Soc Food Science Animal Resources, 2017) Can, Hayriye Yesim; Elmali, Mehmet; Karagoz, AlperThe objectives of this study were: i) to detect the presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk, cheese, beef minced meat, and chicken meat samples; ii) to evaluate the antimicrobial susceptibility of the isolates; and iii) to determine clonal relation among the isolates by using pulsed-field gel electrophoresis (PFGE) method. Therefore, a total of 160 food samples were randomly collected between August 2014 and May 2015 in Hatay province, located in the southern Turkey. Twenty (12.5%) of the samples were found to be contaminated with S. aureus. A total of 40 isolates from the 20 positive samples were confirmed to be S. aureus by multiplex PCR based on 16S rRNA and nuc gene. The mec A gene was not detected in any of the S. aureus strains. In the present study, 39 out of 40 (97.5%) isolates were found to be resistant to one or more antibiotics. All of isolates were susceptible to gentamicin, oxacillin, and vancomycin. The highest resistance rate was detected in penicillin (95%) and ampicillin (92.5%), followed by tetracycline (30%), erythromycin (20%), ciprofloxacin (12.5%). Nine major patterns were determined by PFGE. In 6 of these patterns, thirty-six strains (90%) had identical PFGE profiles.Öğe Observation of Lactic Acid Bacteria and Yeast Populations During Fermentation and Cold Storage in Cow's, Ewe's and Goat's Milk Kefirs(Kafkas Univ, Veteriner Fakultesi Dergisi, 2010) Yaman, Hilmi; Elmali, Mehmet; Kamber, UfukChanges in the population numbers of Lactobacillus spp, Lactococcus + Leuconostoc spp. and yeasts in the kefirs made of cows, ewes and goats milk were determined in this study. The highest count of Lactobacillus spp. was in ewe's milk. There was a slight or no difference in the goats and cow's milk. At 24 h, one log(10) cfu/ml decrease was observed for Lactobacillus spp. in cows and ewes milk. Lactobacillus spp. were more steady in goat milk with a half log(10) cfu/ml decrease. Lactococcus + Leuconostoc spp. similarly reached their maximum populations in cow, ewe and goat milk at the time period of 15-24 h. Lactococcus + Leuconostoc spp. in all milks were in parallel with the maximum populations of Lactobacillus spp. in ewe milk at the time period of 12-15 h. Yeasts had similar numbers in cow, ewe and goat milk at the time period of 15-21 h. Some decreases were observed in the numbers of Lactobacillus spp, Lactococcus + Leuconostoc spp, and yeasts of the kefirs kept at 4 degrees C for 7 days. The decreases of Lactobacillus spp. in cows' and goats' milk kefirs were slight and similar, while one and half log(10) cfu/ml decrease was counted in ewes' milk kefir. The decrease in the numbers of Lactococcus + Leuconostoc spp. was more pronounced than those of Lactobacillus spp. and yeasts. pH decreased during the fermentation and all remained constant over the storage period of 7 days. The type of milk had an influence on the population development of kefirs. Ewe's milk supported the growth of Lactobacillus spp. and Lactococcus + Leuconostoc spp. better. This indicates that different milks may influence the population development of kefir microflora which may affect the quality of kefir.Öğe Occurence and antimicrobial resistance of Arcobacter species in food and slaughterhouse samples(Soc Brasileira Ciencia Tecnologia Alimentos, 2017) Elmali, Mehmet; Can, Hayriye YesimThe objectives of this study were: i) to isolate Arcobacter species (Arcobacter butzleri, Arcobacter skirrowii, Arcobacter cryaerophilus) from different foods and sources, ii) to verify the isolates by multiplex PCR assay, iii) to detect the antibiotic resistance profiles of the isolates. In this study a total of 60 Arcobacter isolates were obtained. Arcobacter species were mostly isolated from swab samples (40%), followed by wastewater (29.1%), broiler wing meat (30%), raw milk (23.9%) and minced meat (6.6%). Regarding the seasonal distribution of Arcobacter from swab and wastewater samples, the bacterium was commonly isolated from wastewater in winter and spring, while it was frequently detected in swab samples during autumn and spring. All of the isolates were found to be resistant to nalidixic acid, ampicillin, rifampin, and erythromycin. The most effective antibiotic was tetracycline, because 96.66% of the isolates were susceptible against it. This is the first report of the isolation, seasonal distribution and antimicrobial susceptibility of Arcobacter species in cattle slaughterhouse samples in Turkey. These results indicate that foods of animal origin and cattle slaughterhouses are significant source of the antimicrobial resistant arcobacters.Öğe Prevalence of Listeria monocytogenes in poultry meat(Soc Brasileira Ciencia Tecnologia Alimentos, 2015) Elmali, Mehmet; Can, Hayriye Yesim; Yaman, HilmiThe objectives of this study were: i) to isolate Listeria spp. and Listeria monocytogenes in broiler wing meat samples, ii) to confirm the isolates by PCR, based on prs and hly A gene sequences, iii) to determine the seasonal and monthly distribution of the isolates. A total of 120 broiler wing meat samples (60 packaged pieces wrapped using strech film in styrofoam plates and 60 unpackaged pieces) bought from different markets in Hatay province were analysed. Listeria spp. was isolated from 57 (47.5%) out of 120 samples. Fifty-four, out of 57 Listeria spp. isolates were identified as L. monocytogenes. L. monocytogenes was isolated from the samples collected during the spring, winter, summer, and autumn at the levels of 26.6%, 40%, 53.3%, 60%, respectively. In this study, the isolation rates were found to be the highest in autumn, while the isolation rates were found to be the lowest in spring. As a consequence, high prevalence of Listeria spp. and L. monocytogenes in poultry wing meat samples may pose a risk for human health. We consider that with obeying the rules of good hygiene practices (GHP), good manufacturing practices (GMP) and HACCP can minimize the contamination with Listeria spp.Öğe The prevalence, vancomycin resistance and virulence gene profiles of Enterococcus species recovered from different foods of animal origin(Univ Zagreb Vet Faculty, 2018) Elmali, Mehmet; Can, Hayriye Y.In this study, Enterococcus faecium was the most commonly found species with a level of 10.1%, followed by Enterococcus durans (19/246, 7.7%), Enterococcus faecalis (13/246, 5.2%), and Enterococcus hirae (9/246, 3.6%). When the virulence gene profile of isolates was evaluated, gelE was the predominant (25/66, 37.8%) virulence factor in isolates followed by asa1 (22/66, 33.3%), esp (12/66, 18.1%), and cylA (4/66, 6.0%). None of the isolates harboured the hyl gene. In this study, all and/or the majority of the Enterococcus isolates tested were found to be resistant to ampicillin, rifampicin, vancomycin, and erythromycin. However, vancomycin resistance genes, such as vanB and vanC1, were not determined in any of the isolates by multiplex PCR. Only three isolates of E. durans recovered from Surk cheese were found to be carrying the vanA gene. The results demonstrate that antimicrobial-resistant and virulent strains of enterococci occur in food of animal origin in Turkey.Öğe Psychrotrophic properties, toxigenic characteristics, and PFGE profiles of Bacillus cereus isolated from different foods and spices(Univ Federal Santa Maria, 2022) Can, Hayriye Yesim; Elmali, Mehmet; Karagoz, Alper; Disli, Huseyin BurakBacillus cereus is an aerobic and facultatively anaerobic, spore-forming bacterium, and it is found naturally in soil and poses a risk factor for the contamination of food and foodstuffs including cereals, vegetables, spices, ready-to-eat (RTE) foods, meats, milk, and dairy products. This study determined the prevalence of B. cereus in raw poultry meat, raw cow's milk, cheese, spices, and RYE foods in Hatay province. The study also analysed the psychrotrophic properties, toxigenic characteristics, and pulsed : field gel electrophoresis (PFGE) profiles of the isolates. The levels of contamination with B. cereus determined for cheese, raw milk, RTE foods, spices, and raw poultry meat were 16.6%, 34.2%, 42.8%, 49%, and 55.5%, respectively. B. cereus was isolated from 84 (42%) of the 200 samples analysed and the 84 isolates were verified by PCR analysis targeting the haemolysin gene specific for B. cereus. Of the total isolates, 64 (76.1%) were psychrotrophic. The toxin gene profiling of B. cereus isolates was determined by amplifying the four genes nhe, hbl, cytK, and ces. The nhe and cytK genes were most frequently detected in the isolates, while the hbl and ces genes were not found. In addition, a high genetic relationship between the isolates was detected at a 92%) similarity level by PFGE analysis. In conclusion, the occurrence of both psychrotrophic and tarigenic B. cereus strains in this study indicated a potential risk for food spoilage and food poisoning.
