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Öğe Determination of dynamic thiol-disulfide levels in dairy cattle with foot disease(Univ Zagreb Vet Faculty, 2022) Deveci, Mehmet Zeki Yilmaz; Erdal, HuseyinFoot diseases are among the top three causes of economic loss in dairy cattle. Recent studies show that oxidative stress plays a critical role in the pathogenesis of foot diseases. There is no study determining the dynamic thioldisulfide levels in dairy cattle with foot disease. This study aimed to determine dynamic thiol-disulfide levels in foot diseased and healthy dairy cattle. Demographic information about the animals, and clinical findings of foot diseases and lameness were presented. In the Foot Disease Group, native thiol (P<0.01), total thiol (P<0.05), disulfide (P<0.01), disulfide/native thiol (P<0.01) and disulfide/total thiol (P<0.01) results were found to be significantly higher than in the Control Group. Native thiol/total thiol (P<0.01) was found to be significantly higher in the Control Group than in the Foot Disease Group. As a result, statistically significant increases in thiol-disulfide levels were determined in foot diseased dairy cattle. It was shown that the thiol-disulfide balance was impaired. This study is the first to determine thiol-disulfide levels and thus changes in thiol-disulfide homeostasis in healthy and foot diseased dairy cattle. With further studies, oxidative stress changes that occur as a result of foot diseases can be better understood and the use of antioxidants as a part of treatment evaluated.Öğe Determination of Serum Perfluorooctanoic Acid and Perfluorooctanesulfonic Acid Levels with Different Stages of Chronic Kidney Disease(Wolters Kluwer Medknow Publications, 2021) Erdal, Huseyin; Sungur, Sana; Koroglu, Muaz; Turgut, FarukThe aim of this study is to investigate the perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) levels in patients with different stages of chronic kidney disease (CKD). Sixty-one CKD stage 1-4 patients who applied to the nephrology outpatient clinic were recruited. A control group consisting of 26 age- and sex-matched healthy controls were also included in the study. Concentrations of PFOA and PFOS were determined by comparing their peak areas with their standard curves. All samples were analyzed three times. The average values of blank samples were subtracted from the detected PFOA and PFOS values. PFOA and PFOS levels were significantly higher in CKD group than the controls (11.4 +/- 7.47, 0.45 +/- 0.55; 0.13 +/- 0. 17, 0.19 +/- 0.4 ng/mL, respectively) (P = 0.001). Hemoglobin, serum albumin, and estimated glomerular filtration rate (eGFR) levels were significantly lower and potassium and uric acid levels were higher in the CKD group than the controls. PFOA and PFOS levels were significantly higher in all stages of CKD patients than healthy controls. However, there was no correlation between eGFR, and PFOS and PFOA. We have demonstrated significantly increased PFOA and PFOS concentrations in different stages of CKD patients. We could not find an association between eGFR, age, and serum PFOS and PFOA concentrations.Öğe THE EFFECT OF PNEUMATIC TUBE SYSTEMS ON THE HEMOLYSIS OF BIOCHEMISTRY BLOOD SAMPLES(Elsevier Science Inc, 2017) Cakirca, Gokhan; Erdal, HuseyinIntroduction: Pneumatic tube systems (PTSs) are widely used in many hospitals because they lead to reduced turnaround times and cost efficiency. However, PTSs may affect the quality of the blood samples transported to the laboratory. The aim of this study was to investigate the effect of the PTS used in our hospital on the hemolysis of the biochemical blood samples transported to the laboratory. Methods: A total of 148 samples were manually transported to the laboratory by hospital staff, 148 samples were transported with the PTS, and 113 were transported with the PTS without use of sponge-rubber inserts (PTSws). Hemolysis rates and the levels of biochemical analytes for the different transportation methods were compared. Results: No significant difference was found between the samples transported manually and with the PTS with regard to hemolysis rate and the levels of biochemical analytes. However, the samples transported with the PTSws showed a significant difference compared with the samples transported manually and with the PTS with regard to hemolysis rate and potassium and lactate dehydrogenase levels. The percentages of the samples that exceeded the permissible threshold for the hemolysis among the samples transported manually, with the PTS, and with the PTSws were 10%, 8%, and 47%, respectively. Discussion: A PTS can be used safely for transporting biochemistry blood samples to the laboratory. However, a sponge-rubber insert that holds sample tubes must be used with the PTS to prevent the hemolysis of blood samples.Öğe Evaluation of dynamic thiol-disulfide homeostasis and ischemia-modified albumin levels in patients with chronic lymphocytic leukemia(Sage Publications Ltd, 2023) Erdal, Huseyin; Ciftciler, Rafiye; Tuncer, Sibel Cigdem; Ozcan, OguzhanThis is the first study to evaluate both the dynamic thiol-disulfide homeostasis and ischemia-modified albumin (IMA) levels in patients with chronic lymphocytic leukemia (CLL). Twenty-nine patients with CLL and 20 controls were included in the study. The dynamic thiol-disulfide balance was determined by the newly developed colorimetric method by Erel. IMA levels were determined by the cobalt binding test. We found that total antioxidant status levels were lower while total oxidant status (TOS) and oxidative stress index (OSI) levels were significantly higher in patients with CLL than controls. Moreover, native and total thiol levels were found to be statistically significant between the study and control groups (p<0.001), whereas no statistically significant difference was noted for IMA levels (p=0.365). A negative correlation was observed between native and total thiol levels, leukocyte, lymphocyte, and TOS. Total bilirubin showed positive correlation with direct bilirubin and alkaline phosphatase. In addition, IMA levels showed a positive correlation with OSI. This study highlights measurement of native and total thiol and IMA levels in patients with CLL for the first time. Dynamic thiol-disulfide homeostasis may contribute in the pathophysiological mechanism, and follow-up to disease in patients with CLL.Öğe Investigation of thiol/disulfide homeostasis in familial mediterranean fever patients(Derman Medical Publ, 2018) Cakirca, Gokhan; Celik, Muhammet Murat; Erdal, Huseyin; Neselioglu, Salim; Erel, Ozcan; Basarali, Mustafa Kemal; Cakirca, Tuba DamarAim: To determine the thiol/disulfide homeostasis in patients with familial Mediterranean fever (FMF) and its correlation with the levels of inflammatory markers consisting of white blood cell count, erythrocyte sedimentation rate, C-reactive protein and fibrinogen. Material and Method: This study was performed in the internal Medicine department of Mustafa Kemal University Hospital in Turkey. A total of 27 FMF patients in the attack period (AP), 33 FMF patients in the attack-free period (AFP), and 36 healthy controls participated in this study. Thiol/disulfide profile parameters were detected using the novel method of Erel and Neselioglu. Results: Total and native thiol levels of the FMF-AP group were markedly lower than those of healthy controls, while the difference in disulfide level was not statistically significant. Thiol/disulfide levels in the FMF-AFP group were similar to the levels in both the FMF-AP group and healthy controls. Correlation analysis showed a negative correlation between fibrinogen levels and total and native thiol levels, while there was a positive correlation between white blood cell count and disulfide levels in the FMF-AP group. Discussion: The findings suggest that decreased concentrations of total and native thiol in patients with FMF-AP are likely to be an outcome of inflammation-induced oxidative stress.Öğe Plasma Apelin Levels and Thiol/Disulfide Balance in Patients with Type 2 Diabetes Mellitus(Aves, 2023) Gunes, Umran Gezici; Turgut, Faruk; Erdal, Huseyin; Dogan, SerdarObjective: The main objective of this research is to investigate the relationship between the plasma apelin concentrations, oxidative stress biomarkers (dynamic thiol/disulfide balance), and albuminuria in patients with type 2 diabetes mellitus. Methods: The study was carried out with 87 patients with type 2 diabetes mellitus and 24 age- and sex-matched healthy control group. Serum apelin concentrations were studied using the enzyme-linked immunosorbent assay method. Colorimetric method was used to determine native and total thiol levels. Concentrations of spot urine albumin and creatinine were measured to calculate the albumin creatinine ratio (mg/g). Results: Serum apelin concentrations were significantly lower in patients with type 2 diabetes mellitus compared to the control group (P <.001). Native and total thiol ratios were also significantly lower in diabetic patients compared to healthy patients (P <.001). The calculated disulfide levels of the patients and control groups were similar (P =.182). A negative and osignificant correlation was detected between serum apelin concentrations, and glucose and hemoglobin A1c levels in diabetic patients (r = -0.272, P =.004, r = -0.280, P =.003, respectively). A negative and significant correlation was also observed between native and total thiol levels and albumin (r = -0.338, P =.001, r = -0.328, P =.001, respectively). Conclusion: We found significantly lowered serum apelin concentrations and native and total thiol levels in patients with type 2 diabetes mellitus. An association was also observed between serum apelin concentrations and glycemic control. The role of apelin and thiol/disulfide balance in diabetic kidney disease requires more detailed studies.Öğe Plasma Ischemia-Modified Albumin Levels and Dynamic Thiol/Disulfide Balance in Sickle Cell Disease: A Case-Control Study(Galenos Yayincilik, 2018) Ozcan, Oguzhan; Erdal, Huseyin; Ilhan, Gul; Demir, Damla; Gurpinar, Ahmet Burak; Neselioglu, Salim; Erel, OzcanObjective: Sickle cell disease (SCR described as a group of inherited blood disorders, affects millions of people throughout the world and is particularly common in the southern part of Turkey. We aimed to determine the relationship between ischemia-modified albumin (IMA) and the dynamic thiol/disulfide balance in SCD. Materials and Methods: Fifty-four adult SCD patients and 30 healthy controls were included in the study. The 54 adult patients included 30 (56% males and 24 (44%/) females with a mean age of 28.3 +/- 8.4 years (minimum-maximum: 18-46 years). Of the 54 patients, 46 had homozygous sickle cell anemia (HbSS) and 8 had sickleni/beta-thalassemia (HbS/(beta(+)-thalassemia). Fasting blood samples were collected. After centrifugation at 1500xg for 10 min, plasma samples were portioned and stored at -80 degrees C. IMA levels were determined by albumin cobalt binding test, a colorimetric method. Total and native thiols and disulfide were analyzed with a novel spectrophotometric method. Results: We found significantly lower levels of native thiol (-SH) (284.0 +/- 86.3 mu mol/L), disulfide levels (14.6 +/- 7 pmol/L), and total thiols (-SH + -S-S-) (313.0 +/- 89.3 ilmol/L) in SCD patients compared to healthy controls (respectively 417.0+54.2, 22.7+11.3, and 462.0 +/- 58.7 mu twill.). Plasma albumin levels (34.9 +/- 7.9 g/L) were lower and IMA levels (13.6 +/- 3.1 g/L) were higher in SCD patients compared to controls (respectively 43.5 +/- 3.1 and 8.4 +/- 1.6 g/L). Plasma albumin levels were strongly correlated with both plasma native (r=0.853; p=0.0001) and total thiols (r=0.866; p=0.0001). Conclusion: Decreased plasma native and total thiol levels and increased IMA levels are related to increased oxidative stress and provide an indirect and quick reflection of the oxidative damage in SCD patients.Öğe The Relationship Between Duration of Hemodialysis, Helicobacter pylori and CagA Seropositivity in Patients with Chronic Kidney Disease(Bilimsel Tip Yayinevi, 2020) Ozcan, Oguzhan; Erdal, Huseyin; Ozer, Burcin; Kacmaz, Filiz; Turgut, FarukIntroduction: The aim of this study was to investigate the relationship between Helicobacter pylori occurrence and, Cytotoxin-associated gene A (CagA) seropositivity with hemodialysis (HD) duration, Serum C-reactive protein (CRP) and hemogram parameters in hemodialysis patients. Materials and Methods: This study included 165 prevalent HD patients. Blood samples were collected before dialysis treatment of the patients. The samples were then centrifuged at 1500 x g for 10 min. immediately after collection. The presence of serum IgG antibody for H. pylori was determined using commercial ELISA kits. Serum anti-CagA IgG antibody of H. pylori positive patients was determined by ELISA method using commercial ELISA kits. CRP levels were measured by nephelometric method, and hemogram parameters were analyzed using fully automated hematology analyzer. Results: We found that the CRP levels of the patients having H. pylori were significantly high. However, there was no significant difference between CRP levels of long-term hemodialysis (LTHD) and short -term hemodialysis (STHD) patients. The possibility of being seropositive of CagA for STHD patients having H. pylori was found to be 2.3 times higher than that of LTHD patients. In addition to that, the periodicity of gastrointestinal (GI) bleeding was significantly high in STHD patients. Conclusion: In conclusion, we found no relationship between dialysis duration and H. pylori prevalence. However, we observed significantly higher odds ratio for CagA seropositivity in patients receiving STHD. These findings suggest that longer dialysis treatment can cause improving effects on H. pylori virulence. Further studies are needed to verify these findings.Öğe Thiol/disulfide homeostasis as a new oxidative stress marker in patients with Fabry disease(Sage Publications Ltd, 2023) Erdal, Huseyin; Turgut, FarukThis is the first study to show both dynamic thiol-disulfide balance and oxidative stress levels in patients with Fabry disease (FD). This prospective study consists of 30 FD patients and 30 healthy controls. Thiol and disulfide values of the study groups were evaluated using a new, cost-effective and fully automatic colorimetric method. A total of 60 subjects were included in the study. A statistically significant difference was found between the patient and control groups for native and total thiol levels (p < 0.001). In addition, disulfide levels were significantly higher in FD patients compared with the control group (p < 0.003). Native thiol levels showed significantly negative correlation with lysosomal globotriaosylceramide, total oxidant status (TOS), and oxidative stress index (OSI) levels. In addition, a positive correlation was found between disulfide/natural thiol and disulfide/total thiol ratios and TOS, OSI, and blood urea nitrogen. We found total antioxidant status levels were lower in the patient group compared to the control group, while TOS and OSI levels were higher and were statistically significant. This study highlights for the first time a novel, cost-effective and fully automated measurement of thiol-disulfide levels in patients with FD. Determination of thiol levels can make important contributions to understand the etiopathogenesis and follow-up of the disease in FD patients.Öğe Thiol/disulfide homeostasis as a new oxidative stress marker in patients with neonatal transient tachypnea(Bayrakol Medical Publisher, 2023) Erdal, Huseyin; Demirtas, Mehmet Semih; Tuncer, Sibel Cigdem; Ozcan, OguzhanAim: Transient tachypnea of the newborn (TTN) is the most common cause of respiratory distress occurring in delayed clearance of lung fluid. This is the first report to examine thiol-disulfide homeostasis in patients with TTN.Material and Methods: Thirty TTN and 30 controls were included in the present study. The dynamic thiol-disulfide balance was determined by the new colorimetric method developed by Erel et al. TAS, TOS and OSI levels were evaluated using the previously described method developed by Erel.Results: Thiol levels were found to be significantly lower between patient and the control groups. However, disulfide levels were not significantly higher in the TTN group compared with the control groups. Disulfide/native thiol, disulfide/total thiol and native/total thiol levels were statistically significantly different between the TTN and control groups. Moreover, we found that TAS, TOS and OSI levels were also statistically significantly different between patient and control groups.Discussion: This study indicates that the measurement of dynamic thiol-disulfide homeostasis may contribute to the pathophysiological mechanism, and follow-up of the disease in patients with TTN. In addition, increased TOS and decreased TAS levels may be related with increased oxidative stress and a functional reduction of antioxidant defense system
