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Öğe Detection by tissue printing of stone fruit viroids, from Europe, the Mediterranean and North and South America(International Society for Horticultural Science, 2004) Torres, H.; Gómez, G.; Pallás, V.; Stamo, B.; Shalaby, Achmed A.; Aouane, B.; Gavriel, I.The two viroids known to affect stone fruit trees are Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd). Viroids can be detected by biological, biochemical or molecular methods. The two first approaches are time consuming and expensive, with the limiting step for molecular techniques being the sample preparation process. In the present study, direct tissue-printing on membranes, a procedure that avoids sample extraction, has been applied on a large scale for an initial screening of PLMVd and HSVd in different regions from Europe (Albania and the Czech Republic), the Mediterranean basin (Algeria, Cyprus, Egypt and Turkey), and North- (Canada) and South-America (Chile). PLMVd was detected in peach trees from all countries surveyed except Egypt, with incidence ranging from 52% (Albania) to 5.4% (Algeria). HSVd was detected in peaches, apricots and plums, in Albania, Egypt and Turkey. The incidence of HSVd in the West part of Turkey both in apricot (66%) and peach (57%) was particularly high.Öğe Efforts to harmonize and promote a stone fruit certification scheme in the Mediterranean countries(International Society for Horticultural Science, 1998) Di Terlizzi, B.; Çaglayan, K.; Gavriel, I.; Myrta, A.; Srhiri, M.; Zeramdini, H.; Audergon, J.M.The awareness of potential disastrous consequences to stone fruit crops from virus diseases (i.e. sharka) has set up alarm signals in the scientific community and has prompted the initiation of national and international projects for the production of virus-free stocks through certification programs. To promote a clean stock program in the Mediterranean region, the Centre International de Hautes Etudes Agronomiques Méditerranéennes (CIHEAM) has promoted the "Mediterranean Network for Virus Disease Assessment and Sanitation of Stone Fruit Trees" (MNFT). Various proposals have been discussed to improve and harmonize current certification schemes starting from EPPO stone fruit protocols now in operation in different countries. Other proposals for more reliable techniques for disease detection to be used in these programs are included. The harmonization of a stone fruit clonal selection and certification scheme in the Mediterranean Region is submitted to the attention of the scientific community and/or the agricultural authorities of member Governments.