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  • Küçük Resim
    Öğe
    Evaluation of the aquaporin molecules characterization in the sperm cells of men from different aged
    (Tubitak Scientific & Technological Research Council Turkey, 2024) Gevrek, Fikret; Gencel, Osman Serden; Gorgun, Selim; Kara, Mikail; Katar, Muzaffer
    Background/aim: Male infertility rises for many reasons, along with age; therefore, we aimed to research the characterization of aquaporin-3, 7, and 8 in human sperm belonging to different age groups. Material and methods: This study was conducted on sperm samples of men aged over 18 years. A total of 60 men were included in the study and divided into three age groups: group 1, age 18-25 years (n = 20); group 2, age 26-35 years (n = 20); and group 3, age >= 35 years (n = 20). Sperm ejaculates obtained from each participant were used for spermiogram tests, Kruger strict morphology analysis, and immunohistochemistry. Results: We observed no statistically significant differences in terms of macroscopic and microscopic sperm testing. The immunostaining score of aquaporin-3 was the lowest in group 1 and increased in group 3 and group 2, respectively (p < 0.05). Aquaporin-8 immunostaining only increased in group 2 (p < 0.05). Aquaporin-7 immunostaining scores were not different between the groups (p > 0.05). When the immunostaining scores of aquaporin molecules were compared with each other, aquaporin-7 was significantly increased compared with the others (p < 0.05). Conclusion: According to the results, it can be stated that aquaporin-3 and aquaporin-8 molecules were more expressed at age 26 to 35 years, and aquaporin-7 was densely expressed from age 18 to 25 years. If the characterization of these molecules is adversely affected, male infertility may eventually emerge. We recommend further advanced -level studies on this subject.
  • Küçük Resim
    Öğe
    Investigation of the sperm aquaporin molecules expression in fertile and infertile men
    (2023) Gevrek, Fikret; Akyoruk, Arif; Kara, Mikail; Katar, Muzaffer; Erdemir, Fikret
    Aim: Infertility is pregnancy failure for a year without protection, despite regular sexual intercourse. Various disorders and deficiencies in both genders can cause this situation, and a considerable portion of these cases consists of male infertility. We aimed to investigate the relationship between aquaporin molecules expressed in human sperm cells and male infertility. Materials and Methods: We carried out the study on the sperm cells of two different groups consisting of ten fertile and twenty infertile men older than eighteen. Smear slides were prepared following spermiogram testing. These slides were stained using the Diffquick and immunohistochemistry protocols to test the Kruger strict sperm morphology and detect the immune expression of the AQP molecules, respectively. Results: The results of the spermiogram and Kruger-strict sperm morphology analysis showed that average sperm cell count and numerical sperm parameters values in the semen of infertile individuals decreased, and sperm counts with anomalies were higher in infertile ones versus those of fertile (p<0.05) as predicted. Our immunohistochemical analysis findings revealed that the immune expression of each aquaporin molecule was reduced slightly in infertile men regarding fertile ones, but the decrease was not statistically significant (p>0.05). Conclusion: According to our findings, the immune expression of infertile ones’ aquaporin molecules was reduced by a small amount, even if this was not statistically meaningful. These molecules of the sperm cells can not be primarily associated with male infertility. However, further advanced-level research is required on this issue to make a precise decision.