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    Öğe
    Investigation of the prophylactic and therapeutic effectiveness of oral thyme extract in rats experimentally infected with cryptosporidium parvum
    (Springer, 2023) Kara, Erdal; Duru, Sibel Yasa; Gokpinar, Sami; Duru, Ozkan; Sevin, Sedat; Senel, Yasin; Kaya, Ufuk
    In this study, the prophylactic and therapeutic activities of thyme extract at different concentrations against experimental Cryptosporidium parvum infection in immunosuppressed rats were investigated. Thyme extract was prepared at four different concentrations (10%, 30%, 50%, and 100%) and administered as a single oral dose of 1 mL for evaluation of its prophylactic efficacy. Five consecutive days after infection was detected in all rats, therapeutic evaluations were also performed. According to the results obtained by daily counting of oocysts in stools, the prophylactic and therapeutic effects of thyme extract administration were significant in comparison to the control group (P<0.01). Oocyst shedding continued in the control group at high numbers from the beginning to the end of the study, while oocyst counts in the prophylaxis groups remained low throughout the study. On the other hand, oocyst excretion rates were high in the therapeutic groups and decreased rapidly after thyme extract administration. At the end of the study, oocyst excretion had completely stopped for some rats administered thyme extract. There was no group in which oocyst shedding ceased for all rats. No significant differences were observed in the therapeutic or prophylaxis groups regarding the doses administered (P > 0.01). Renal and hepatic functions were monitored by measuring urea, creatinine, alanine transaminase, and aspartate transaminase levels before and after thyme extract administration. As a result, it was concluded that oral thyme extract administration at the doses applied in this study is effective and safe in the prophylactic and therapeutic treatment of experimental cryptosporidiosis in rats.
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    Öğe
    A research on Babesia and Theileria species in sheep and goats of Kirikkale province through molecular methods
    (Tubitak Scientific & Technological Research Council Turkey, 2021) Gokpinar, Sami; Gazyagci, Aycan Nuriye; Aydenizoz, Meral; Kaya, Ufuk
    The aim of the present study was to determine presence and prevalence of Babesia and Theileria species in the sheep and goats of stock farming in the province of Kirikkale, which is in Central Anatolia Region of Turkey through microscopic examination and RLB method. Venous blood samples of 3 mL were collected from vena jugularis of 300 sheep and 100 goats into tubes containing EDTA. Blood smears were prepared from samples; and stained by Giemsa and examined for Theileria and Babesia under a light microscope. Genomic DNAs were extracted from blood samples with EDTA through suitable methods; a region with a length of 360 to 430 bp on variable region V4 of the 18 ssu rRNA gene of Theileria and Babesia species were proliferated; PCR products obtained were hybridized on a membrane where species-specific probes. Piroplasms were detected in 54 (18%) sheep and in none of the goats during microscopic examination of blood smears. With the RLB technique; T. ovis 70%, Theileria sp. 2.3%, B. ovis 5.3%, T. ovis + B. ovis 2.7% were detected in sheep. In goats; T. ovis was detected at a rate of 1%. Consequently, this study is the first for detection of Theileria and Babesia species in sheep and goats in Kirikkale. Presence of T. ovis and B. ovis in the sheep, and T. ovis in goats was detected in Kirikkale.