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Öğe Antioxidant activities of some monofloral honey types produced across Turkey(Elsevier, 2018) Gul, Aziz; Pehlivan, TubaThis study was conducted with the aim of determining the chemical, biochemical properties, and antimicrobial capabilities of some of the monofloral honeys produced in Turkey. In this study, 23 different monofloral honey samples were obtained from diverse geographical regions of Turkey. Floral origin of the honey samples was determined by melissopalinological analyses. Additionally, antioxidant properties were determined. To determine the antioxidant properties of honey samples, four test methods of total phenolic content, DPPH, iron reduction power and beta-carotene linoleic acid emulsion method were used. As a result of the antioxidant activity analysis among the honey samples, rhododendron and parsley honey showed most prominent results in terms of the amount of phenolic compounds and antioxidant activity. On the other hand, acacia and citrus honey samples showed least antioxidant activity. A positive correlation was determined between four methods. Differences between antioxidant activities of honey samples were significantly found (P < 0.01). (C) 2018 The Authors. Production and hosting by Elsevier B.V.Öğe Caucasian Honey Bee ( Apis mellifera caucasia) Factors Causing Deterioration of the Gene Center and Suggestions for Solutions(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Gul, Aziz; Nergiz, RamazanThis study was carried out in Ardahan and Artvin cities where Caucasian honeybees were purely bred. In this study, it was aimed to determine the structure of beekeeping, socio-economic quantity, the status of bee diseases and pests, the problems that destroy the Caucasian Bees Gene Center and to offer some solutions. Within the scope of this study, a questionnaire was carried out face to face with 24 beekeepers who have permissions to produce queen bee. According to the obtained data, it has been determined that the mean age of beekeepers is 52.5, their mean experience is 24.3 years. 48.3% of the breeders were graduated from high school, the main job of 35.6% of breeders is beekeeping, 46.1% of them started to beekeeping via buying hive with honeybees, 83.6% of breeders are pleased with queen producing, 79% of them maintain the queens from gene centers and, 100% of breeders were determined to study with cross breed Caucasian Bees. 46.1% of breeders believe that the region is isolated from the other honeybee species, 72.8% of beekeepers reported that precautions were not taken to protect Caucasian bees. 63.3% of them reported that migratory beekeepers came to the region illegally, 76.6% of them reported that illegal entry to the region should be prevented to protect the Gene Center.Öğe Chemical Composition and Preservative Effect of Thrkish Propolis on Egg Quality Durig Storage(Asian Journal Of Chemistry, 2009) Sahinler, Nuray; Gul, Aziz; Copur, GuelsenThe present study was carried out to investigate chemical composition and preservative effects of Turkish propolis on quality of fresh eggs. For this purpose, different concentrations of ethonolic extract of propolis (5, 8, 10 % EEP) were prepared. Total of (9 period x 5 group x 10 n) 450 egg were used during experiment. The eggs were randomly divided into 5 experimental groups. The first, second and third group eggs covered with 5, 8, 10 % EEP, fourth group with alcohol control and fifth group as a control (uncovered), respectively. Ten eggs in each group were drawn randomly and examined each week between 17 March-12 May 2006. Chemical analysis of propolis extracts indicated that the propolis samples had high concentrations of the aromatic acids, esters and other derivatives which are responsible for the antibacterial, antifungal, antiviral, anti inflammatory and anticancer properties of propolis such as benzyl cinnamate, methyl cinnamate, caffeic acid, cinnamyl cinnamate and cinnamoylglcine besides the most common compounds as fatty acid, terpenoids, esters, alcohols hydrocarbons and aromatic acids. It was found that ethanolic extract of propolis improved interior egg quality.Öğe Cryoprotective Effect of Vitamin E Supplementation of Different Extenders on Quality and Fertilizing Ability of Frozen-Thawed Brown Trout Sperm(Mary Ann Liebert, Inc, 2021) Bozkurt, Yusuf; Yavas, Ilker; Bucak, Mustafa Numan; Kiran, Tugba Raika; Gul, AzizVitamin E is one of the most powerful antioxidants for prevention of cell damage resulting from cryopreservation, but its efficacy for cryopreserving brown trout sperm is still unclear. In this work, the protective effect of vitamin E on quality, fertilizing capacity, and DNA damage of brown trout (Salmo trutta macrostigma) sperm after cryopreservation was evaluated. Sperm samples were diluted at the ratio of 1:10 with three different extenders (E): (E-I): 300 mM glucose, 10% egg yolk; (E-II): 33.3 mM glucose, 5.1 mM NaCl, 0.5 mM NaHCO3,, 15% DMA; and (E-III): 61.6 mM NaCl, 134.2 mM KCl, 1.9 mM CaCl2, 0.8 mM MgCl2, 2.3 mM NaHCO3 in distilled water. Each extender was supplemented with 10% DMSO and different concentrations of vitamin E at 0.1, 0.5, and 1.0 mM. Spermatozoa frozen without vitamin E (0 mM, control) and fresh sperm were also used. After dilution, the sperm was aspirated into 0.25 mL straws, frozen 3 cm above the liquid nitrogen (LN2) surface, and plunged into the LN2. Cell motility, viability, fertilization, and eyeing were determined in post-thawed samples. DNA damage was determined by the comet assay after cryopreservation. Supplementation of 1 mM vitamin E to all extenders exhibited the best cryoprotective effect in terms of sperm motility, duration of motility, viability, fertility, and DNA integrity against cryopreservation damage, compared with 0.1, 0.5, and control group (0 mM) (p < 0.05). The highest post-thaw motility (62.4% +/- 0.36%), fertilization (48.2 +/- 0.84), and the lowest DNA damage (7.245%) were obtained with the extender-II including 1.0 mM vitamin E (p < 0.05). Consequently, vitamin E positively affected the motility parameters, fertility, and DNA integrity, and the results suggest the addition of extenders with vitamin E as an antioxidant for the cryopreservation of brown trout sperm.Öğe Determination of Honey Botanical Origin by Using Discriminant Analysis(Medwell Online, 2009) Sahinler, Suat; Sahinler, Nuray; Gul, AzizOne of the most important criterions is determination of honey botanical origin in point of the sense of taste, the force of habit and quality of honey and the results of this, in preference of honey. The purpose of this study, is to discriminate the honey type using a linear discriminant function on the basis of honey chemical characteristics by using discriminant analysis, which is one of the multivariate techniques and has recently been much used in applied sciences. In this study, independent variables, mineral content (%), moisture (%), pH, acidity (meq), invert sugar (%), sucrose (%), diastase level and HMF (mg) belonging to 5 types as sunflower, citrus, cotton, pine and high plateau honeys were used. The discriminant functions from different combinations of variables on the basis of honey chemical characteristics were given and the cases from the original honey botanical origins, sunflower, citrus, cotton, pine and high plateau, could be classified 100% correctly by using these functions.Öğe EFFECT OF EXTENDER SUPPLEMENTED WITH BORON ON POST-THAW MOTILITY, VIABILITY, DNA DAMAGE AND FERTILIZATION ABILITY OF CRYOPRESERVED BROWN TROUT (Salmo trutta macrostigma) SPERMATOZOA(Cryo Letters, 2019) Bozkurt, Yusuf; Yavas, Ilker; Gul, Aziz; Bucak, Mustafa Numan; Yeni, Deniz; Avdatek, FatihBACKGROUND: Boron has been considered as an essential nutrient for decreasing lipid peroxidation and improving antioxidant mechanism in different animal species. On the other hand, its effect on quality or DNA damage following cryopreservation process in fish sperm is still unclear. OBJECTIVE: Experiments were designed to analyse the effect of an ionic based extender supplemented with boron on post-thawed motility, viability, fertility and DNA integrity of cryopreserved brown trout (Salmo trutta macrostigma) sperm. MATERIALS AND METHODS: Sperm samples were cryopreserved with the ionic extender containing different boron concentrations (0.1, 0.2, 0.3 and 0.4 mM) using a controlled freezer at two different freezing rates (FR-I: 10 degrees C min(-1) from +4 degrees C to -40 degrees C and FR-II: 15 degrees C min(-1) from +4 degrees C to -40 degrees C). Sperm motility, viability, fertilization, eyeing and DNA fragmentations were determined in post-thawed samples. RESULTS: Freezing rate-I provided significantly higher fertilization and eyeing rates compared to freezing rate-II (p<0.05). Higher post-thaw motility (62.8 +/- 1.4%) and fertilization (75.2 +/- 0.9%) rates were obtained with the 0.4 mM boron concentration at freezing rate-I. CONCLUSION: Supplementation of the extender with boron increased fertilization and eyeing rates and also decreased DNA damages at both freezing rates.Öğe Effects of diluents and plasma on honey bee (Apis mellifera L.) drone frozen-thawed semen fertility(Elsevier Science Inc, 2017) Gul, Aziz; Sahinler, Nuray; Onal, Ali G.; Hopkins, Brandon K.; Sheppard, Walter S.Cryopreservation is an advanced method used to protect germplasm in liquid nitrogen. Honey bees are of special interest to protect because of their pollination activity and critical role in agriculture. There has been important progress in the cryopreservation of honey bee germplasm in recent years, leading to practical recovery of genetic material for breeding purposes following freezing. However, there remains room for improvement and the goal of the present study was to evaluate the effect of different extenders added post-thaw on the fertilization rate of cryopreserved honey bee semen. The purpose of adding extender post-thaw was to dilute the cryoprotectant to remove chemicals after centrifugation because of potential adverse effects. The control consisted of frozen-thawed semen without the addition of an extender; treatment groups included the addition of one of the following extenders: glucose solution, fresh ram semen plasma, fresh honey bee semen plasma, extender solution. All of the above treatments and frozen-thawed control were compared to fresh semen. For each group, 15 virgin queens were instrumentally inseminated with the semen-diluent solution and introduced into nucleus colonies to determine the brood patterns of the queens. Percentages of worker brood produced in the fresh semen, frozen-thawed semen control, glucose, fresh ram semen plasma, fresh honey bee semen plasma, and extender solution supplemented groups were 98 +/- 1.1%, 47 +/- 0.9%, 3.0 +/- 0.8%, 0.3 +/- 0.1%, 48.1 +/- 4.1% and 40.3 +/- 2.4%, respectively. Similiarly, spermatozoa numbers in the spermathecae of the same treatment groups were 3.6 x 10(6),1.6 x 10(6), 73 x 10(6), 4.7 x 10(6), 8.1 x 10(6), and 4.6 x 10(6) spermatozoa for the same treatment, respectively. The differences in both worker brood percentage and sperm count in the spermatheca were statistically significant (P < 0.01) among all treatment groups, except the frozen thawed control group and fresh drone semen plasma group. We found a positive correlation between sperm count in the spermatheca and the percentage of worker brood (r = 0.91). With the exception of fresh honey bee semen plasma, the fertility rate was reduced following the addition of various plasmas and diluents post-freezing. (C) 2017 Elsevier Inc. All rights reserved.Öğe Effects of Propolis on Storage of Sweet Cherry Cultivar Aksehir Napolyon(Asian Journal Of Chemistry, 2009) Candir, Elif Ertuerk; Ozdemir, Ahmet Erhan; Soylu, Emine Mine; Sahinler, Nuray; Gul, AzizSweet cherries cultivar Aksehir Napolyon were dipped in ethanol extracted propolis (EEP) and water extracted propolis (WEP) in various concentrations immediately after harvest and then kept at 0 degrees C, 85-90 % relative humidity for 4 weeks, The following treatments were applied to the fruits: dipping in water, dipping in ethanol (70 %), dipping in 1, 5 and 10 % concentrations of ethanol extracted propolis and dipping in 1, 5 and 10 % concentrations of water extracted propolis. The effects of propolis on incidence of fungal decay and fruit quality (weight loss, total soluble solids, titratable acidity, skin colour, stem browning, surface pitting, appearance and taste) were assessed at weekly intervals during storage. Ethanol extracted propolis treatments were effective in preventing fungal decay in cherries for 4 weeks, but adversely affected sensory quality and stem colour of cherries.Öğe Evaluation of the standards compliance of the queen bees reared in the Mediterranean region in Turkey(Elsevier, 2021) Arslan, Servet; Cengiz, Mahir Murat; Gul, Aziz; Sayed, SamyThe influence of different commercial queen producers on the quality of Apis mellifera queens was assessed. It was aimed to determine the quality characteristics of queens reared by commercial queen producers located in the province of Antalya, which is an important region in queens production due to its climatic characteristics. For this purpose, the quality characteristics of a total of 105 queen bees obtained from 21 enterprises were determined. Differences between the enterprises in terms of the number of spermatozoa (P < 0.01) were determined. In terms of the diameter of spermatheca, spermatheca volume and live weight, statistical differences between the enterprises were also observed (P < 0.05). When the relationships between the measured characteristics were examined, significant values were obtained statistically between live weight and diameter of spermathecae (0.268) and spermatheca volume (0.258). It was also determined that there is a significant correlation between spermatheca diameter and spermatheca volume (0.995). The spermatheca diameter of a good quality queen bee should not be <1.2 mm, spermatheca volume 0.90 mm(3) and live weight not <200 mg. Only live weight was found to be within the normal quality standard values when the average results of the quality criteria are taken into consideration. Other characters such as spermathecae diameter, spermathecae volume and number of spermatozoa in spermathecae seem to be below quality standard values. (C) 2021 The Author(s). Published by Elsevier B.V.Öğe Using AHP and PROMETHEE multi-criteria decision making methods to define suitable apiary locations(Taylor & Francis Ltd, 2020) Sari, Fatih; Kandemir, Irfan; Ceylan, Durmus Ali; Gul, AzizBeekeeping activities have a rapidly increasing importance due to their derived products and their contributions to human health, biodiversity, agriculture, and pollination. Moreover, beekeeping can play a major role in supporting rural development and bringing the benefits of sustainable developments and productivity. Thus, the decision on correct apiary locations via suitability analysis techniques seems essential to sustain and increase the yield and efficiency. However, this decision-making process requires a large number of parameters including the topography of the field surrounding environment and climate conditions. At this point, Multi Criteria Decision Analysis techniques can provide efficient solutions for decision making on suitable apiary locations. In this study, suitable apiary site selection analysis for beekeeping activities via Analytical Hierarchy Process (AHP) and The Preference Ranking Organization Method for Enrichment of Evaluations (PROMETHEE) methods was proposed for Konya city located in Central Anatolia as a pilot application. Aspect, elevation, slope, flora, water resources, roads, railroads, settlements, electricity lines, buildings, precipitation, and natural disaster area criteria were assessed to generate beekeeping suitability for apiaries. The restrictions and requirements of beekeeping activities were specified considering expert beekeeper decisions and recent studies for the study area. The AHP and PROMETHEE suitability maps were validated with existing 461 apiary locations to be able to determine the reliability and applicability.
