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    PCR/RFLP-based cost-effective identification of SOD2 signal (leader) sequence polymorphism (Ala-9Val) using NgoM IV: a detailed methodological approach
    (Elsevier, 2004) Akyol, O; Canatan, H; Yilmaz, HR; Yuce, H; Ozyurt, H; Sogut, S; Gulec, M
    Background: Superoxide dismutases (SOD) play an important role in the protection of cells and extracellular space from the products of oxidative stress. Two allelic variants have been described for the SOD2 gene (Ile58Thr involves a C to T substitution at nucleotide residue 339 and Ala-9Val involves a T to C substitution at nucleotide residue 1183). The enzyme proteins encoded by the different alleles have been suggested to have different activity patterns. Methods: The SOD2 polymorphism was determined using a polymerase chain reaction (PCR) and RFLP techniques with restriction endonuclease NgoM IV Results: The most available results were obtained from with 20 pmol primer final concentration in PCR reaction. A total of 20 pmol seems the cost-effective primer concentration with maximum quality. There were no difference between the band quality of 1-5 units of restriction endonucleases. On the other hand, short and long incubation times seem to be similar in order to obtain sharp bands on agarose gel. Conclusions: We have extended a method of SOD2 polymorphism (Ala-9Val) in mitochondrial sequence. This method provides the ability to genotype of SOD2, and it represents a fast, reliable, cost-effective and semi-automated methodology to determine SOD2 polymorphism in order to perform large-scale population studies. (C) 2004 Elsevier B.V. All rights reserved.
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    Protective role of ?-tocopherol and caffeic acid phenethyl ester on ischemia-reperfusion injury via nitric oxide and myeloperoxidase in rat kidneys
    (Elsevier, 2004) Gurel, A; Armutcu, F; Sahin, S; Sogut, S; Ozyurt, H; Gulec, M; Kutlu, NO
    Background: The aim of this study was to determine the acute effects of antioxidant caffeic acid phenethyl ester (CAPE) and alpha-tocopherol (vitamin E) on nitric oxide (NO) production, neutrophil infiltration, and antioxidant enzyme activities on an in vivo model of renal ischemia-reperfusion injury. Methods: Rats were divided into five equal groups each consisting six rats: sham operation, ischemia, ischemia-reperfusion (I/R), I/R plus CAPE, and I/R plus vitamin E groups. CAPE or vitamin E was administered intraperitoneally before reperfusion. After experimental procedure, rats were sacrificed and both ipsilateral and contralateral kidneys were removed and prepared for NO concentrations, myeloperoxidase (MPO), catalase (CAT) and superoxide dismutase (SOD) activities. Results: Acute administration of vitamin E decreased NO concentrations in both ipsilateral and contralateral renal tissues compared to I/R group. SOD activity was increased in I/R and I/R + CAPE groups compared to sham operation group. The most prominent results were encountered in MPO activities, which did not change in contralateral kidneys in both ischemia and I/R groups. There was a significant decrease in ipsilateral MPO activity in ischemia group and a significant increase in I/R group compared to sham operation group. Pretreatment with intraperitoneal CAPE significantly diminished the tissue MPO activity indicating the prevention of the neutrophil sequestration into the kidney. Conclusion: There is a role for CAPE in attenuation in renal damage after I/R injury of the kidney, in part at least by inhibition of neutrophil sequestration. (C) 2003 Elsevier B.V. All rights reserved.
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    The regulatory role of dietary ?-3 essential fatty acids on oxidant/antioxidant balance in rat hippocampus
    (John Wiley & Sons Ltd, 2003) Sarsimaz, M; Songur, A; Kus, I; Ozyurt, B; Gulec, M; Sogut, S; Ilhan, A
    Omega-3 essential fatty acids (omega-3 EFA) contains eicosapentaenoic acid and docosahexaenoic acid (DHA). DHA is one of the building structures of membrane phospholipids of brain and necessary for continuity of neuronal functions. omega-3 EFA has been suggested to be protective against neuropsychiatric disorders including schizophrenia. This study proposed to assess the changes in superoxide dismutase (SOD), xanthine oxidase (XO), malondialdehyde (MDA), and nitric oxide (NO) in the hippocampus of rats fed with omega-3 EFA diet (0.4 g/kg/day) for 30 days. Eight control rats and nine rats fed with omega-3 EFA were decapitated under ether anesthesia, and hippocampus was removed immediately. Rats treated with omega-3 EFA had significantly lower XO activity (p<0.002) and NO level (p<0.0001) whereas higher SOD activity (p<0.002) and MDA levels (p<0.019) than the control rats. These results suggest that the dietary omega-3 EFA may act on the oxidant/antioxidant parameters in hippocampus. On the other hand, although the mechanism is not clear, omega-3 EFA may enhance one of the most important antioxidant enzymes, SOD. Further studies are needed to clarify the molecular mechanism involved and the therapeutic implication of omega-3 EFA in animal psychosis models and clinical studies.