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Öğe Demodex Parazytes in Schizophrenia(Derman Medical Publ, 2016) Kokacya, Mehmet Hanifi; Hamamci, Berna; Copoglu, Umit Sertan; Kaya, Ozlem AycanAim: Demodex parazytes are commonly present all over the world, especially in facial region of humans. Demodex spp. are assumed to be more common in schizophrenia due to partial suppression of immune system and lack of good self-care. The present study aimed to investigate the prevalence of Demodex ectoparasites in schizophrenia patients. Material and Method: In the study, 31 patients with a diagnosis of schizophrenia and 30 subjects without any psychiatric disorder or skin disease were subjected to standard superficial skin biopsy technique to determine Demodex spp. Results: Demodex spp. were found positive in nine schizophrenia patients and it was found positive in two healthy controls. Considering the prevalence of Demodex spp., a significant relationship is found between schizophrenia patients and normal controls (p< 0.05). Discussion: As a result, Demodex spp. are more common in schizophrenia due conditions of reduced self-care and immunosuppression, Demodex parasites should be considered in schizophrenia patients with skin lesions, especially on the face, and should to be treated if needed.Öğe Determination of Ethanol in Blood Samples Using Partial Least Square Regression Applied to Surface Enhanced Raman Spectroscopy(Springer Heidelberg, 2018) Acikgoz, Gunes; Hamamci, Berna; Yildiz, AbdulkadirAlcohol consumption triggers toxic effect to organs and tissues in the human body. The risks are essentially thought to be related to ethanol content in alcoholic beverages. The identification of ethanol in blood samples requires rapid, minimal sample handling, and non-destructive analysis, such as Raman Spectroscopy. This study aims to apply Raman Spectroscopy for identification of ethanol in blood samples. Silver nanoparticles were synthesized to obtain Surface Enhanced Raman Spectroscopy (SERS) spectra of blood samples. The SERS spectra were used for Partial Least Square (PLS) for determining ethanol quantitatively. To apply PLS method, 920-820 cm(-1) band interval was chosen and the spectral changes of the observed concentrations statistically associated with each other. The blood samples were examined according to this model and the quantity of ethanol was determined as that: first a calibration method was established. A strong relationship was observed between known concentration values and the values obtained by PLS method (R-2 = 1). Second instead of then, quantities of ethanol in 40 blood samples were predicted according to the calibration method. Quantitative analysis of the ethanol in the blood was done by analyzing the data obtained by Raman spectroscopy and the PLS method.Öğe Determination of ethanol in blood samples using partial least square regression applied to surface enhanced Raman spectroscopy (vol 34, pg 127, 2018)(Korean Soc Toxicology, 2024) Acikgoz, Guenes; Hamamci, Berna; Yildiz, Abdulkadir[Abstract Not Available]Öğe Determination of ethyl glucuronide (EtG) in blood samples using partial least squares discriminant analysis applied to surface-enhanced Raman spectroscopy(Elsevier, 2020) Acikgoz, Gunes; Hamamci, BernaConsumption of alcohol and problems related to alcohol vary widely around the world. Especially illegally produced and distributed alcohols have a great risk for human health, since they include high quantity of toxic substances. Ethanol measurements have high specificity for excessive alcohol intake. Ethyl Glucuronide (EtG) is considered as a direct biomarker of recent ethanol consumption. The study aims to identify EtG in blood samples by using Raman Spectroscopy. Silver colloidal nanoparticles were synthesized to obtain SERS (Surface-Enhanced Raman Spectroscopy) spectra of blood samples and EtG. PLS-DA (Partial Least Squares Discriminant Analysis) models were built on the collected SERS spectra. It is observed that EtG and the blood samples taken from people using alcohol are in the same group and the blood samples taken from no alcohol users are in a separate cluster from EtG. The sources of data variability are modelled by the so-called Latent Variables (LVs). The percentage of LVs were found to be 29.53% and 8.34%, respectively. In the study, it has been observed that the level of EtG changes according to the level of ethanol in blood samples. However, there is no study on the detection of EtG in blood with Raman spectroscopy. Outcomes of the present study encourage the feasibility of the application of Raman Spectroscopy for determination of EtG in blood samples.Öğe The Distribution of Intestinal Parasites Determined Between 2009-2010 in Erciyes University, Medical Faculty, Parasitology Laboratory(Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Cetinikaya, Ulfet; Yazar, Suleyman; Kuk, Salih; Ates, Serpil; Hamamci, Berna; Gedikbas, Tugba; Sahin, IzzetThe infection caused by intestinal parasites infects through the mouth are common in the world. The intestinal parasites are a health problem not only developing countries but also developed country. They cause various symptoms such as abdominal pain, diarrhea, weight loss, anemia, and growth retardation. The prevalence of intestinal parasites is affected by various factors such as climate, socioeconomic status, age, sex, sanitation, and hygiene. In this study, we aimed the distribution of intestinal parasites in patients presenting with intestinal complaints to Erciyes University Medical Faculty, Parasitology department. This study carried out from January 2009-December 2010. A total of 16.445 stool samples were examined using native-Lugol and sedimentation methods and 1.482 cellophane tape preparations were examined directly. Totally 3.214(19.5%) patients, 1.745 (10.6%) men and 1.469 (8.9%) women were found infected with one or more intestinal parasite species. The more prevalent parasites were found as Blastocystis hominis 2.602 (15.8%), Entamoeba coli 351(2.1%) and Giardia intestinalis 299 (1.9%) respectively.Öğe Investigation of Fasciola hepatica Seropositivity in forensic autopsies in the Çukurova region(Turkiye Klinikleri, 2013) Zeren, Cem; Eren, Ali; Çelikel, Adnan; Köksaldi Motor, Vicdan; Yengil, Erhan; Hamamci, BernaObjective: Fasciola hepatica is a parasite that affects ruminant animals and is rarely seen in humans. The agent may cause significant health problems and economic loss as a zoonotic disease. Freshwater plants play an important role for infecting humans with this parasite. Eating watercress, drinking water that contains metacercaria, using contaminated kitchen tools, or consuming green vegetables may cause infestation. Green vegetables and fruits, especially watercress, are grown specifically in the Cukurova region and this puts the population living in this region at risk of developing fasciolosis. For the reason of absence of the seroprevalence studies in autopsies and to determine the prevalence of this region, the aim of this study, is to investigate F. hepatica antibody by the method of IHA blood samples which were taken from autopsy cases in the Çukurova Region. Material and Methods: This study was conducted in Adana Forensic Medicine Department, a center where all forensic autopsies from the cities of Adana, Mersin, Kahramanmaras, Hatay and Osmaniye were performed. There were 94 subjects between the ages of 2-87 years (mean:42.55±SD22.09) and their serum samples were collected for assessment of F. hepatica antibodies via the IHA method. Moreover, we examined the hepatobiliary system for adult parasites. Results: IHA results demonstrated that 13 (13.8%) of the subjects were seropositivite for F. hepatica. During the autopsy, however, mature F. hepatica was not observed in hepatobiliary systems of the subjects. Seropositivity rates in Adana, Mersin, Hatay and Osmaniye were 19.4%, 7.1%, 12.5% and 22.2%, respectively. Conclusion: To our knowledge, this is the first study evaluating F. hepatica seropositivity in forensic autopsies. Seeing the results, we suggest that more comprehensive epidemiologic research is needed to study this infestation, especially in endemic areas. © 2013 by Türkiye Klinikleri.Öğe Investigation of Trichomonas Vaginalis by Parasitological and Cytological Methods in Patients Admitted to Gynecology Outpatient Clinics in a University Hospital(Duzce Univ, 2015) Aycan-Kaya, Ozlem; Benk-Silfeler, Dilek; Ozgur, Tumay; Keskin-Kurt, Raziye; Hamamci, Berna; Sefil, Nebihat; Bayazit, AhmetAim: In this study, it was aimed to compare parasitological and cytological diagnostic methods to detect T. Vaginalis in samples taken from posterior fornix of vagina. Methods: In this cross-sectional study, study population (n= 104) were selected from women applying to gynecology outpatient clinic of Mustafa Kemal University Hospital with different complaints between March-August 2013. The presence of T. vaginalis was investigated by parasitological and cytological diagnostic methods. While direct microscopy, giemsa staining and culture in Cysteine-Peptone-Liver-Maltose medium (CPLM) were performed for parasitological diagnosis of T. vaginalis in samples taken from posterior fornix of vagina, cervical smear stained by Papanicolaou (PAPS) was used for cytological diagnosis. Results: T. vaginalis was detected in 12 (11.5%), 12 (11.5%), 14 (%13.4) of materials by direct microscopy, giemsa staining and culture, respectively. T. vaginalis was detected only 5 (4.8%) of materials by cytology. This difference was statistically significant (p<0.05). Conclusion: Our study results showed that parasitological methods are more sensitive than cytological methods in diagnosis of T. vaginalis. Therefore, T. vaginalis should be screened in women with non-specific symptoms in under developed countries where lack of periodic examination and polygamy were seen.Öğe Molecular genotyping of Echinococcus granulosus sensu stricto from human Echinococcal cysts in Hatay, Turkiye(Academic Press Inc Elsevier Science, 2023) Hamamci, Berna; Acikgoz, Gunes; Cetinkaya, Uelfet; Kilic, Erol; Kocal, Sedat; Karaaslan, Kerem; Yetim, Tuelin DurgunCystic echinococcosis (CE) is one of the zoonotic infections in human, an important global health problem. It was aimed to determine the molecular characterization and phylogenetic analysis of isolates obtained from patients diagnosed with CE in Hatay province, according to the cox1 gene region. A total of 31 patients, 14 males and 17 females, with a mean age of 35.19 (+/- 14.28) years were included in the study. 35 cyst materials obtained from patients were studied. DNA isolation was performed from the samples with protoscoleces determined in the cyst fluid. One-way DNA sequencing was performed with the Sanger Sequencing Protocol through the obtained PCR products. In the study, 35 hydatid cysts of human origin were examined and protoscoleces was detected in 11 (31.43%) of them. Twenty of the patients had liver involvement, seven had lung involvement, and four had both liver and lung involvement. All the samples with protoscoleces detected were observed of PCR product with a size of approximately 446 bp. When the sequence results of the isolates were evaluated within themselves, it was seen that there were three different sequences with 99% similarity to each other. As a result, of the phylogenetic analysis, it was determined that the isolates were identified in the Echinococcus granulosus sensu stricto (E. granulosus s. s.) (G1-G3) complex. This study is thought to contribute to the epidemiology, parasite control, effective diagnosis and treatment techniques, eradication, vaccine and drug development studies of E. granulosus s. s in Turkiye.Öğe Prevalence of Encephalitozoon intestinalis and Enterocytozoon bieneusi in Cancer Patients Under Chemotherapy(Ankara Microbiology Soc, 2015) Hamamci, Berna; Cetinkaya, Ulfet; Berk, Veli; Kaynar, Leylagul; Kuk, Salih; Yazar, SuleymanMicrosporidia species are obligate intracellular parasites and constitute one of the most important opportunistic pathogens that can cause severe infections especially in immunocompromised patients. Enterocytozoon bieneusi and Encephalitozoon intestinalis are the most common species among 14 microsporidia species identified as human pathogens. The aim of this study was to investigate the prevalence of E.intestinalis and E.bieneusi in cancer patients under chemotherapy by immunofluorescent antibody and conventional staining methods. A total of 123 stool samples obtained from 93 patients (58 male, 35 female) with cancer who were followed in oncology and hematology clinics of our hospital and 30 healthy volunteers (13 male, 17 female) were included in the study. Fifty-one (55%) of the patients had complain of diarrhea. The presence of E.intestinalis and E.bieneusi were investigated by a commercial immunofluorescence antibody test using monoclonal antibodies (IFA-MAbs; Bordier Affinity Products, Switzerland) in all of the samples, and 50 of the samples were also investigated by modified trichrome, acid-fast trichrome and calcofluor staining methods. A total of 65 (69.9%) patients were found positive with IFA-MAbs method, including 43 (46.2%) E.intestinalis, 9 (9.7%) E.bieneusi and 13 (14%) mixed infections. In the control group, 5 (16.7%) subjects were positive with IFA-MAbs method, including 2 (6.7%) E.intestinalis, 1 (3.3%) E.bieneusi and 2 (6.7%) mixed infections. The difference between the positivity rate of the patient and control groups was statistically significant (p< 0.05). Of the patients with diarrhea, 68.6% (35/51) were infected with microsporidia, and the difference between cases with and without (48.6%) diarrhea was statistically significant (p< 0.05). When 50 samples in which all of the methods could be performed were evaluated, the frequency of microsporidia were detected as follows; 66% (n= 33) with IFA-MAbs, 34% (n= 17) with modified trichrome staining, 24% (n= 12) with acid-fast trichrome staining and 42% (n= 21) with calcofluor staining methods. Our data indicated that the use of IFA-MAbs method along with the conventional staining methods in diagnosis of microsporidia will increase the sensitivity. As a conclusion, the prevalence of E.intestinalis and E.bieneusi in cancer patients under chemotherapy was detected quite high (69.9%) in our study, it would be appropriate to screen these patients regularly in terms of microsporidian pathogens.
