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Öğe Effect of auxin type and concentrations in different media on the callus induction and shoot formation of crested wheatgrass (Agropyron cristatum (L.) Gaertn)(Diagnosis Press Ltd, 2008) Can, E.; Celiktas, N.; Hatipoglu, R.Effects of different medium (LS and SH).supplemented with various auxin concentrations (2, 4, 6 and 8 mg. l(-1)) of dicamba (3,6-dichloro-2metoxibenzoic acid) and picloram (4-amino-3,5,6-trichloropicolinic acid) on the callers induction, direct shoot foramtion rate and callus weight from young inflorescences crested wheatgrass (Agropyron cristatum (L.) Gaertn). The result of the study showed that callus induction rate (`%), shoot formation (%) and callus weight (mg/per Petri dishes) the young inflorescences were signficantly; affected by medium, auxin and auxin concentrations. Depending on the medium, mean callus,induction rate,.shoot formation and callus weight (mg/per Petri dishes) varied from 18% -18.8%, 8.6% - 62.5%, 11.4 - 16.4 mg, respectively Callus induction,.shoot formation rates and callers weight were also significantly influenced by the auxin ripe and auxin concentrations. The highest callus induction rate and callers weight were obtained from the LS medium containing 4 mg. P t(-1) dicamba. The highest direct shoo! formation rate was obtained from the SH medium containing 2 mg. P of picloram..Öğe Effect of inflorescence length on the callus induction and plant regeneration in orchardgrass (dactylis glomerata l.)(2003) Çeliktas, N.; Can, E.; Hatipoglu, R.This study was carried out to determine the effect of inflorescence length on callus induction and plant regeneration in Orchardgrass (Dactylis glomerata L.). In the study, the inflorescences with different length (2–25 mm) isolated from the ecotype with a good in-vitro regeneration capacity were cultured on LS medium supplemented with 5 mg?1 2,4-D. Both callus induction and directly shoot regeneration from the inflorescences of orchard-grass were observed during the investigation. Callus induction rates varied from 50% to 84.5% depending on the inflorescence length. The highest callus weight per inflorescence was obtained from the inflorescence with 11–15 mm length. The inflorescences being longer than 15 mm produced lower callus weight than those being shorter than 15 mm. The highest regeneration rate (4.4 plantlets per inflorescence) was obtained from the inflorescences with the 11–15 mm length. Increasing the inflorescence length up to 20 mm decreased the regeneration rate. Considering the results of study, it was concluded that the inflorescences being yellowish in color and 6–20 mm length could be used as explant for an acceptable regeneration rate. © 2003 Taylor and Francis Group, LLC.Öğe Effects of genotype and concentrations of dicamba on callus induction and plant regeneration from young inflorescences of perennial ryegrass (lolium perenne l.)(2004) Can, E.; Celiktas, N.; Hatipoglu, R.; Yilmaz, S.; Avci, S.This study was carried out to determine the effects of genotypes and dicamba concentrations (2.5, 5, 7.5 and 10 mg?1) on the callus induction and plant regeneration from the segments of young inflorescences cultured on the LS-medium in perennial ryegrass (Lolium perenne L). The result of the study showed that callus induction rate, callus weight per petri dish and plant regeneration from the young inflorescences were significantly affected by the genotypes. Depending on the genotypes, callus induction rate, callus weight mg/petri dish and number of regenerates per inflorescence segment varied from 20.3 %-67.2 %, 54.4–118.1, 0.797–2.719 respectively. Callus and shoot induction rates, callus weight and regeneration rate were also significantly influenced by the dicamba concentrations. The segments cultured on the LS medium containing 5 mg?1 of dicamba gave the highest values of callus induction rate (77.1 %), shoot induction rate (66.6 %), callus weight (168.2 mg/petri dish) and regeneration rate (3.458 regenerates per segment). © 2004 Taylor and Francis Group, LLC.Öğe NEW MULTIVARIATE EMPIRICAL PREDICTION EQUATION FOR RELATIVE FEED VALUE OF NATIVE GRASSES(Aloki Applied Ecological Research And Forensic Inst Ltd, 2020) Celiktas, N.; Can, E.; Kaya, S.; Atis, I; Hatipoglu, R.; Ertekin, IRelative feed value and structural mineral differentiation of native grasses and their relationships at different plant growth stages were studied to create a more informative multivariate model to predict relative feed value. The hierarchical clustering grouped the species and their growth stages under six distinct categories with their average relative feed values of 122.6, 108.6, 99.6, 90.3, 80.9 and 71.5. The principal component analyses for the relative feed value and the mineral composition of native grasses was efficient to classify the forages with the total explained variation of 63.69% with the first two principal components. The most important predictors for relative feed value were determined as nitrogen and potassium contents of the native grasses according to beta coefficients from the partial least square regression analyses. Three partial least square regression based new empirical equations for predicting the relative feed value were constructed by using the forage nitrogen content. The coefficients of determination (R-2) and the root mean square error of prediction (RMSEP) for the equations were 0.92, 0.35, 0.81 and 2.17, 11.29, 5.88, respectively. The Fisher's F test manifested that the actual and the predicted relative feed values were not different (P>0.05) for all three equations.
