Yazar "Ilhan, E." seçeneğine göre listele

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    ANALYSIS OF ONOBRYCHIS GENETIC DIVERSITY USING SSR MARKERS FROM RELATED LEGUME SPECIES
    (Pakistan Agricultural Scientists Forum, 2014) Avci, S.; Ilhan, E.; Erayman, M.; Sancak, C.
    Availability of legume microsatellite markers for Onobrychis taxa was limited. However, cross genera utilization of such markers has been of great interest due to the high cost and labor. In the present study, we attempted to transfer microsatellite markers from Phaseolus vulgaris L. and Medicago truncatula Gaertn. to Onobrychis genus. Additionally, transferred markers were used to identify genetic diversity among Onobrychis taxa collected from different regions of Turkey. Of the 95 SSR primer pairs previously used for P. vulgaris and M. truncatula, 18 primers were successfully amplified and showed polymorphism among 58 Onobrychis taxa. Eighteen SSR primers observed 79 loci resulting in 725 alleles. The highest number of loci was obtained from BM175 and MTIC84 primers. Gene diversity and polymorphism information content values showed that P. vulgaris primers produced the most informative loci on Onobrychis genomes. The highest genetic diversity values were obtained for Onobrychis argyrea Boiss. subsp argyrea Boiss. (53) while the lowest from Onobrychis cornuta (L.) Desv.(1). The average diversity values were the highest on Hymenobrychis section which was followed by Heliobrychis, Onobrychis, Laphobrychis and Dendobrychis sections. Magnitude of genetic variation was the highest within Onobrychis section in which genetic similarity values ranged from 0.013 to 0.399. The SSR and phylogenetic analysis results showed that sections were separated similar to their morphological characteristics. However, Hymenobrychis and Heliobrychis clearly separated from other sections. Our study showed that Onobrychis genomes could be successfully studied using other legume SSR markers. Therefore, they can be used for conservation of Onobrychis species as well as improving new varieties for feed use.
  • [ N/A ]
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    Analysis of Onobrychis genetic diversity using SSR markers from related legume species
    (Pakistan Agricultural Scientists Forum, 2014) Avci, S.; Ilhan, E.; Erayman, M.; Sancak, C.
    Availability of legume microsatellite markers for Onobrychis taxa was limited. However, cross genera utilization of such markers has been of great interest due to the high cost and labor. In the present study, we attempted to transfer microsatellite markers from Phaseolus vulgaris L. and Medicago truncatula Gaertn. to Onobrychis genus. Additionally, transferred markers were used to identify genetic diversity among Onobrychis taxa collected from different regions of Turkey. Of the 95 SSR primer pairs previously used for P. vulgaris and M. truncatula, 18 primers were successfully amplified and showed polymorphism among 58 Onobrychis taxa. Eighteen SSR primers observed 79 loci resulting in 725 alleles. The highest number of loci was obtained from BM175 and MTIC84 primers. Gene diversity and polymorphism information content values showed that P. vulgaris primers produced the most informative loci on Onobrychis genomes. The highest genetic diversity values were obtained for Onobrychis argyrea Boiss. subsp argyrea Boiss. (53) while the lowest from Onobrychis cornuta (L.) Desv.(1). The average diversity values were the highest on Hymenobrychis section which was followed by Heliobrychis, Onobrychis, Laphobrychis and Dendobrychis sections. Magnitude of genetic variation was the highest within Onobrychis section in which genetic similarity values ranged from 0.013 to 0.399. The SSR and phylogenetic analysis results showed that sections were separated similar to their morphological characteristics. However, Hymenobrychis and Heliobrychis clearly separated from other sections. Our study showed that Onobrychis genomes could be successfully studied using other legume SSR markers. Therefore, they can be used for conservation of Onobrychis species as well as improving new varieties for feed use.
  • [ N/A ]
    Öğe
    Determination of genetic characterization and bioethanol yield of selected maize lines and cultivars
    (Wiley-Blackwell, 2016) Erayman, M.; Horuz, M.; Konuskan, O.; Ilhan, E.; Eren, A. H.
    [Abstract Not Available]
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    Hexaploid wheat (Triticum aestivum) root miRNome analysis in response to salt stress
    (Wiley, 2015) Eren, H.; Pekmezci, M. Y.; Okay, S.; Turktas, M.; Inal, B.; Ilhan, E.; Atak, M.
    Hexaploid bread wheat (Triticum aestivum) is one of the major crops grown and consumed all over the world. Elevated soil salinity causes reduction in crop yield and quality; therefore, several strategies were developed to improve salt-tolerant cultivars. MicroRNAs (miRNAs), small and non-coding RNAs, regulate gene expression at post-transcriptional level and play important roles in stress tolerance. Here, we used a broad-range miRNA-microarray analysis to investigate the root-miRNA profiles of two cultivars, Bezostaja (sensitive) and Seri-82 (tolerant). A total of 44 differentially regulated miRNAs were identified in the 8 x 15K array containing 11 862 plant miRNAs available in the database. Sixteen novel salt-responsive miRNAs were determined in wheat for the first time. The expression of three miRNAs (hvu-miR5049a, ppt-miR1074 and osa-miR444b.2) was up-regulated more than 260-fold in cv. Bezostaja upon salt stress. The target-gene analyses showed that several salt-stress-responsive miRNAs regulate mainly transcription factors such as bHLH135-like, AP2/ERBP, MADS-box and transporters. Gene ontology searches for 565 putative salt-stress-responsive miRNA target-genes revealed 623 processes in 10 different main topics such as metabolic process and response to stimuli. The genome-wide root miRNome study indicates salt-stress-responsive wheat miRNAs and the possible mechanisms behind the tolerance.
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    WATERLOGGING AND NITRIC OXIDE INDUCE GENE EXPRESSION AND INCREASE ANTIOXIDANT ENZYME ACTIVITY IN WHEAT (TRITICUM AESTIVUM L.)
    (Akademiai Kiado Zrt, 2014) Ozcubukcu, S.; Ergun, N.; Ilhan, E.
    The effects of waterlogging (WL) and WL plus nitric oxide (WL+NO) were investigated in seedlings of one wheat cultivars (Triticum aestivum cv. Dogankent) and one wheat line (Triticum aestivum cv. Ducula-4). Under WL conditions, catalase activity was greater in Ducula-4 than in Dogankent. Glutathione reductase activity increased in Ducula-4 seedlings under WL+NO conditions, especially at 48 and 72 hours of treatment. Myb2 expression increased during the early hours of treatment in both wheat varieties exposed to WL, with 40-fold higher levels in Ducula-4, gradually decreasing to control levels. Under WL+NO treatment, Myb2 expression increased 44-fold at 12 hours and high levels of expression were still observed at 72 hours. When Ducula-4 seedlings were subjected to WL+NO treatment, PDPK expression increased approximately 15-fold at 3 hours and decreased to control levels at 72 hours. Under the same conditions, SST1 expression increased 3-fold at 3 and 12 hours and reached control levels during the subsequent hours. Among the genes studied, the highest level of expression was observed for Myb2. Moreover, gene expression was altered most by waterlogging in Ducula-4 seedlings.