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    Investigation of antioxidant effect of silibinin molecule on U-2 OS cells induced by hydrogen peroxide
    (2022) Gündüz, Kübra; Ecevit, Hasret; Küçük, Meral Urhan; Dırıcan, Emre
    Aim: Silibinin is one of the active constituent of silymarin, an extract of the Silybum\rmarianum L. seeds. There are previous studies that have reported the antioxidant, antiinflammatory, anti-apoptotic and anti-carcinogenic effects of silibinin. However, the antioxidant effect of silibinin on bone cancer have been not evaluated before. In our study,\rwe aimed to evaluate the effect of silibinin on U-2 OS cell proliferation and oxidative stress\rparameters catalase enzyme activity and MDA level.\rMaterials and Methods: U-2 OS cells were exposed to H2O2 (0-800 µM H2O2, for\r24 and 48 hours) to form an oxidative damaged cell model. Moreover, the cells were exposed to silibinin (0-100 µM) for 24 hours. Malondialdehyde (MDA) level and antioxidant\renzyme catalase (CAT) activity were determined by spectrophotometric analysis.\rResults: CAT activity and MDA level were observed to be higher in both H2O2 exposure\rgroups compared to control group (p<0.05). Moreover, CAT activity and MDA level were\robserved to be lower in 500 µM H2O2+silibinin groups in comparation with 500 µM H2O2\rgroup (p<0.05). Similarly, MDA level was determined to be lower in 650 µM H2O2+10\rµM silibinin group in comparation to 650 µM H2O2 group (p<0.05).\rConclusion: In conclusion, it was observed that silibinin molecule has anti-proliferative\reffect and decreases catalase enzyme activity and MDA level in a concentration dependent\rmanner.