Yazar "Karaaslan, Kerem" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • [ N/A ]
    Öğe
    Effects of surgical and percutaneous tracheotomy on thyroid hormones
    (Edizioni Luigi Pozzi, 2019) Yetim, Tulin Durgun; Ozer, Gul Soylu; Karaaslan, Kerem; Yetim, Brahim; Kilic, Erol
    Aim: To evaluate the effects of surgical and percutaneous tracheotomy on thyroid hormones. MATERIAL AND METHOD: Sixty patients with respiratory problems who underwent surgical tracheotomy and percutaneous tracheotomy between December 2012 and December 2016 were divided into 2 groups. FT3, FT4, thyroglobulin and TSH levels of the groups were statistically evaluated preoperatively and postoperatively. RESULTS: The effects of surgical and percutaneous tracheotomy on free thyroxin (FT4), serum thyroglobulin (TG) and thyroid stimulating hormone (TSH) levels were found to be statistically significant. Although free triiodothyronine (FT3) slightly elevated in both groups, it was not statistically significant. DISCUSSION: Today, percutaneous tracheotomy (PCT) and conventional surgical tracheotomy (CT) have been widely used in intensive care units on patients who are expected to be connected to mechanical ventilation for a long time. Because of the anatomy of the surgical site, tracheotomy may cause damage to the adjacent thyroid gland and tracheal rings CONCLUSION: Surgeons should keep in mind that serum thyroid hormone levels may increase postoperatively. Particularly the patients with cardiac rhythm problems should be followed after surgical and percutaneous tracheotomy due to the systemic effects of thyroid hormones.
  • Küçük Resim
    Öğe
    Ekinokokkozis’te germinal membran ve kist sıvısının raman spektroskopisi ile moleküler düzeyde biyokimyasal analizi
    (Türkiye Parazitoloji Derneği, 2019) Hamamcı, Berna; Açıkgöz, Güneş; Kılıç, Erol; Karaaslan, Kerem; Çetinkaya, Ülfet; Durgun Yetim, Tülin; Yetim, İbrahim
    Amaç: Hydatidosis, Echinococcus granulosus’un larva evresinin neden olduğu zoonotik paraziter bir enfeksiyondur. Çalışmada, karaciğer tutulumu olan hastaların cerrahi sırasında elde edilen germinal membran ve kist sıvılarının Raman spektroskopisi ile moleküler düzeyde biyokimyasal yapılarının incelenmesi amaçlanmıştır. Yöntemler: Çalışmada, germinal membran ve kist sıvılarının mitokondriyal gen bölgesine göre moleküler karakterizasyonları belirlenmiş ve filogenetik analizleri yapılmıştır. Örneklerde Raman spektroskopisi kullanılarak 300-1800 cm-1 arasındaki spektral bantlar incelenmiştir. Bulgular: PZR analizleri sonucunda hastalardan elde edilen germinal membranlarda ve kist sıvılarında yaklaşık 400 bç büyüklüğünde DNA bandı elde edilmiştir. Germinal membranda 780, 880, 970, 1151, 1200, 1270 cm-1’de, kist sıvısında 780 ve 1200 cm-1’de farklı pikler gözlenmiştir. 1333-1335 cm-1’de elde edilen en şiddetli spektral bantların CH3CH2 kollajen ve polinükleotit zincirini gösteren modlar olduğu belirlenmiştir. Bu ise, E. granulosus’un moleküler düzeyde biyokimyasal yapısını gösteren spesifik pik olarak değerlendirilmiştir. Sonuç: Mikroorganizmaların tanımlanmasında ve biyolojik dokuların biyokimyasal analizlerinde; moleküler, serolojik ve konvansiyonel farklı tanı yöntemleri kullanılmaktadır. Bu yöntemlere ek olarak Raman spektroskopisinin hızlı, tahribatsız ve noninvaziv bir yöntem olduğu yapılan çalışmada gösterilmiştir. Dolayısıyla mikroorganizmaların temel biyokimyasal bileşenlerinin moleküler düzeyde analiz edilebilmesi bakımından alternatif bir yöntem olacağı düşünülmektedir.
  • Küçük Resim
    Öğe
    Molecular genotyping of Echinococcus granulosus sensu stricto from human Echinococcal cysts in Hatay, Turkiye
    (Academic Press Inc Elsevier Science, 2023) Hamamci, Berna; Acikgoz, Gunes; Cetinkaya, Uelfet; Kilic, Erol; Kocal, Sedat; Karaaslan, Kerem; Yetim, Tuelin Durgun
    Cystic echinococcosis (CE) is one of the zoonotic infections in human, an important global health problem. It was aimed to determine the molecular characterization and phylogenetic analysis of isolates obtained from patients diagnosed with CE in Hatay province, according to the cox1 gene region. A total of 31 patients, 14 males and 17 females, with a mean age of 35.19 (+/- 14.28) years were included in the study. 35 cyst materials obtained from patients were studied. DNA isolation was performed from the samples with protoscoleces determined in the cyst fluid. One-way DNA sequencing was performed with the Sanger Sequencing Protocol through the obtained PCR products. In the study, 35 hydatid cysts of human origin were examined and protoscoleces was detected in 11 (31.43%) of them. Twenty of the patients had liver involvement, seven had lung involvement, and four had both liver and lung involvement. All the samples with protoscoleces detected were observed of PCR product with a size of approximately 446 bp. When the sequence results of the isolates were evaluated within themselves, it was seen that there were three different sequences with 99% similarity to each other. As a result, of the phylogenetic analysis, it was determined that the isolates were identified in the Echinococcus granulosus sensu stricto (E. granulosus s. s.) (G1-G3) complex. This study is thought to contribute to the epidemiology, parasite control, effective diagnosis and treatment techniques, eradication, vaccine and drug development studies of E. granulosus s. s in Turkiye.