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    Chemical composition, antimicrobial and antibiofilm efficacy of C. limon and L. angustifolia EOs and of their mixtures against Staphylococcus epidermidis clinical strains
    (Elsevier Science Bv, 2018) Danila, Elena; Moldovan, Zenovia; Popa, Marcela; Chifiriuc, Mariana Carmen; Kaya, Alpaslan Durmus; Kaya, Madalina Albu
    Staphylococcus epidermidis is a Gram-positive resident of the human skin, which in certain conditions, can turn into an opportunistic pathogen, being one of the most frequent agents of nosocomial infections, particularly of those associated with indwelling medical devices. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of two essential oils (EOs) to be used alone or in combination as natural alternatives to currently available antibiotics, for treating S. epidermidis infections or preventing the contamination and further development of S. epidermidis biofilm on viable tissues or medical devices. For this purpose C. Limon and L. angustifolia EOs were obtained both by hydrodistillation and microwave-assisted extraction and analyzed by GC MS. The compositions of EOs as a function of the extraction procedure were compared and discussed. Each of the two extraction techniques was more effective in the extraction of different components of the EOs. In the case of C. limon EOs, terpinen-4-ol, alpha-terpineol and linalool, not found in the microwave assisted extract, are obtained by classical hydrodistillation (being compounds of high polarity) while less polar compounds (alpha-pinene, beta-pinene, limonene, gamma-terpinene) are drawn into higher percentage through the microwave-assisted procedure. In the case of L. angustifolia EOs, a higher percentage of compounds with high polarity (such as linalool) is extracted by classical hydrodistillation while the compounds with lower polarity, such as acetates (linalyl acetate and neryl acetate) are extracted in higher proportion by the microwave-assisted extraction method. The antibacterial and antibiofilm effects of the EOs and of their mixtures were tested towards five S. epidermidis clinical strains and their biological activity was correlated with the oils composition. The most active composition was represented by the binary mixture (1:1) of the EOs obtained by hydrodistillation, containing terpene alcohols (such linalool) and D-limonene, which exhibited the lowest minimum inhibitory concentration (3.9 mu g/mL) and minimum biofilm eradicating concentration (0.97-7.81 mu g/mL), against all tested S. epidermidis strains. Other minor constituents, such as terpinen-4-ol, alpha-terpineol, linalyl acetate, neryl acetate, geranyl acetate and a-pinene could be potentiated in presence of limonene and could be responsible for the high antimicrobial activity of these EOs mixture.
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    Comparative Study of Lavandula angustifolia Essential Oils Obtained by Microwave and Classical Hydrodistillation
    (Chiminform Data S A, 2018) Danila, Elena; Kaya, Durmus Alpaslan; Patrascu, Mariana; Kaya, Madalina Albu; Kumbakisaka, Sylviu
    Lavandula angustifolia which belong to Lamiaceae family with origins in the Mediterranean regionis one of the most important species of Lavandula. The most common method for obtaining the essential oil of lavender is described in the literature is steam hydrodistillation using a Neo-Clevenger, official method described in the European Pharmacopoeia. Another method described in the literature for obtaining oil of lavender is the extraction using microwave. The aim of this study was to obtain lavender essential oils bythese two different methods: classical hydrodistillation using Neo-Clevenger apparatus and microwave-assisted extraction. The yield of extraction was different for the two of methods 0.124 % for classical hydrodistillation and 0.6 % for microwave - assisted extraction. In order to establish and compare the chemical composition of lavender essential oils we used a gas chromatography coupled with mass spectrometry (GC-MS). In the classical hydrodistillation method 44 components with 99.20% yield and in microwave extraction, 46 components with 99.33% yields have been identified. The main compound of essential oil obtained was Linalool. But the other secondary main components are totally different. Even if the obtainedyield for lavender essential oil was bigger in the case of classical hydrodistillation, taking into account the time of extraction was lower for the oil obtained by microwave, it is recommended to continue with this modern method.
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    COMPOSITE SCAFFOLDS FOR BONE REGENERATION MADE OF COLLAGEN/HYDROXYAPATITE/EUCALYPTUS ESSENTIAL OIL
    (Incdtp-Icpi, 2018) Florea, Andrei Dan; Danila, Elena; Constantinescu, Rodica Roxana; Kaya, Madalina Albu; Kaya, Alpaslan Durmus; Coara, Gheorghe; Albu, Luminita
    Bone regeneration is a serious problem nowadays because of the increased number of people suffering from infections, arthritis and bond loss. The aim of the present study was to develop and characterize collagen - hydroxyapatite - essential oil matrices for bone regeneration. In our study we wish to develop biomaterials which mimic bone composition and prevent allergic or toxic effects. The matrices obtained by freeze-drying were characterized by FT-IR analysis, water uptake capacity and microbiological analysis. The results obtained from analyses confirmed that collagen-hydroxyapatite-essential oils matrices exhibit proper characteristics for bone regeneration.
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    Novel Nanocomposite Hydrogels Based on Crosslinked Microbial Polysaccharide as Potential Bioactive Wound Dressings
    (Mdpi, 2023) Marin, Maria Minodora; Kaya, Madalina Albu; Kaya, Durmus Alpaslan; Constantinescu, Roxana; Trica, Bogdan; Gifu, Ioana Catalina; Alexandrescu, Elvira
    A multitude of dressings have been developed to promote wound repair, such as membranes, foams, hydrocolloids and hydrogels. In this study, a crosslinked polysaccharide hydrogel was mixed with a bioactive ingredient to synthesize a novel nanocomposite material to be used in wound healing. Variation of the ratio between hydrogel components was followed and its effect was analyzed in regard to swelling, degradation rate and thermo-mechanical behavior. The resulting crosslinked structures were characterized by FTIR and microscopy analyses. The antimicrobial activity of the crosslinked hydrogels loaded with bioactive agent was evaluated using two bacterial strains (Gram-positive Staphylococcus aureus and Gram-negative bacteria Escherichia Coli). All the results showed that the new synthesized biopolymer nanocomposites have adequate properties to be used as antibacterial wound dressings.
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    OBTAINING AND CHARACTERIZATION OF SOME EMULSIONS BASED ON COLLAGEN HYDROLYSATE AND NATURAL EXTRACTS WITH A POTENTIAL ANTICELLULITIC ACTION
    (Polytechnic Univ Bucharest, 2019) Danila, Elena; Stan, Raluca; Enache, Adelina Elena; Turkmen, Musa; Kaya, Durmus Alpaslan; Kaya, Madalina Albu; Serafim, Andrada
    The aim of this study was to formulate and evaluate O/W emulsions based on collagen and naturals ingredients such as vegetable oils and butters, caffeine, ivy extract and pink pepper essential oil. The essential oil of pink pepper was obtained by hydrodistilation and characterized by GC-MS. The main components of this essential oil are: alpha-Phelandrene 35.84%, Limonene 17.31%, alpha-Pinene 1.98% (monoterpenes) and beta-Phelandrene 13.04%, o-Cymene 4.65%, delta-Cadinene 3.27%, trans-Caryophyllene3.04%, Bicyclogermacrene 2.13% (sesquiterpenes). The emulsions obtained are stable and the pH values of emulsions correspond to the natural pH of the skin, indicating that emulsions can be safely applied to the skin. Results from the optical microscopy analysis show that emulsions have a creamy and foam like appearance. All the emulsions present adequate Theological properties. Further microbiological tests and other analysis are necessary for the obtained emulsions.
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    Polymer – flufenamic acid delivery systems for injured skin
    (2022) Marin, Maria Minodora; Ghıca, Mihaela Violeta; Kaya, Durmuş Alpaslan; Udeanu, Denisa Ioana; Kaya, Madalina Albu; Dinu-Pirvu, Cristina-Elena; Popa, Lacramıoara
    Collagen, the main protein of the body, is extracted in different forms and used as reservoir for drug delivery. The aim of this work was to obtain a drug delivery system based on collagen-dextran matrices cross-linked with glutaraldehyde as support and flufenamic acid and/or microcapsules with flufenamic acid as drug. The flufenamic acid was encapsulated in polymeric microcapsules consisting in gelatin, alginate, and sodium carboxymethyl cellulose. The morphology of matrices was determined by water absorption and contact angle. The biodegradation was performed in collagenase solution. In vitroflufenamic acid release profiles were built and the kinetic mechanism was set according to different mathematical models. The pharmacological studies followed the effect of collagen formulations treatment on the healing process of Wistar rats which were induced experimental wounds. The studied matrices proved that flufenamic acid delivery can be controlled, and the healing can be completed using the designed spongious matrices.