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    Comparing the effects of fluticasone, anti-IgE and anti-TNF treatments in a chronic asthma model
    (Elsevier Doyma Sl, 2018) Ozkars, M. Y.; Keskin, O.; Tokur, M.; Ulasli, M.; Gogebakan, B.; Ciralik, H.; Kucukosmanoglu, E.
    Background: Corticosteroids are used in the treatment of asthma. The aim of this study was to determine the efficacy of anti-IgE and anti-TNF alpha as asthma treatments. Methods: A mouse model of chronic asthma was developed. The fluticasone group was exposed to fluticasone and the anti-IgE and anti-TNF groups were administered anti-IgE or anti-TNF. IL-4, and IgE levels were measured, and histological analysis, pathological analysis and miRNA-126, miRNA-133a analyses were applied. Results: The cell concentration in the BAL fluid decreased in all the treatment groups. The rate of perivascular and peribronchial cell infiltration decreased in the lung in the high-dose anti-IgE and anti-TNF groups. Smooth muscle thickness decreased in the lung tissue in the low dose anti-IgE and anti-TNF groups. Bronchial wall thickness decreased in the lung tissue in the fluticasone + antidgE group. The IL-4 level in BAL fluid decreased in the high-dose anti-IgE, fluticasone + anti-IgE and anti-TNF groups. IgE levels increased in the BAL fluid in the high dose anti-IgE and anti-TNF groups. The lymphocyte level increased in the BAL fluid in the high-dose anti-IgE group. The macrophage level decreased in the BAL fluid in the anti-TNF group. The relative expression of miRNA-126 increased in all groups. The relative expression of miRNA-133a decreased in the placebo and fluticasone groups. The relative expression of miRNA133a increased in the low-dose anti-IgE, high-dose anti-IgE, fluticasone + anti-IgE and anti-TNF groups. Conclusions: The results showed that anti-IgE is successful as a treatment. Fluticasone + antiIgE and anti-TNF were seen to be superior to other therapeutic modalities when used for prophylaxis. (C) 2017 SEICAP. Published by Elsevier Espana, S.L.U. All rights reserved.
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    Effects on cells in the BAL fluid of asthma medications
    (Wiley-Blackwell, 2015) Ozkars, M. Y.; Keskin, O.; Tokur, M.; Gogebakan, B.; Ciralik, H.; Kucukosmanoglu, E.; Demirel, C.
    [Abstract Not Available]
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    Öğe
    Effects on histological changes on the BAL fluid of asthma medications
    (Wiley-Blackwell, 2015) Ozkars, M. Y.; Keskin, O.; Tokur, M.; Gogebakan, B.; Ciralik, H.; Kucukosmanoglu, E.; Demirel, C.
    [Abstract Not Available]
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    Öğe
    Effects on IL-4 and IgE on the BAL fluid of asthma medications
    (Wiley-Blackwell, 2015) Ozkars, M. Y.; Keskin, O.; Tokur, M.; Gogebakan, B.; Ciralik, H.; Kucukosmanoglu, E.; Demirel, C.
    [Abstract Not Available]
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    Öğe
    Effects on miRNA on the lung tissue of asthma medications
    (Wiley-Blackwell, 2015) Ozkars, M. Y.; Keskin, O.; Tokur, M.; Gogebakan, B.; Ciralik, H.; Kucukosmanoglu, E.; Demirel, C.
    [Abstract Not Available]
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    Öğe
    Molecular Characterization of Staphylococcus aureus from Clinical Sheep Mastitis Cases
    (Israel Veterinary Medical Assoc, 2022) Aslantas, O.; Keskin, O.; Yucetepe, Gullu A.
    In the current study, it was aimed to examine the genes encoding toxin, biofilm and microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) of 62 Staphylococcus aureus isolated from sheep clinical mastitis cases using polvmerase chain reaction (PCR). Besides, accessory gene regulator (agr) groups and the antibiotic susceptibilities of the isolates were also determined. Most of the isolates (87.1%) were susceptible to all tested antimicrobials. The sec (38, 61.3%) and sell (50, 80.6%) were the only staphylococcal enterotoxin (SE) genes detected among the isolates. The tst gene was observed in 43 (57.3%) isolates. Whereas co-existence of sec, sell, tst was detected in 36 (48%) isolates; none of them isolates harbored eta and eth genes. However, eight (12.9%) isolates were found to be negative for SE, ET and TSST-1 genes. Hemolysin genes were detected in all isolates and the frequency of bla,hlb,hld, and hlg2 were 100%, 95.2%, 98.4%, and 61.3%, respectively. The lukED and lukM were found together in 40 (64.5%) of the isolates, and /ukED was found alone in 22 (35.5%) of the isolates. Of the biofilm-related genes, icaD was the only gene detected with a frequency of 83.9% (52). MSCRAMMs genes eno, cna, ebps, fib, fnb, clfA, and c()13 were detected in 100%, 79%, 100%, 95.2%, 100%, and 100%, respectively. None of the isolates carried the bbp gene. This study indicated that S. aureus isolates from clinical sheep mastitis possessed several virulence-associated determinants.