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    Anoxybacillus calidus sp nov., a thermophilic bacterium isolated from soil near a thermal power plant
    (Microbiology Soc, 2014) Cihan, Arzu Coleri; Cokmus, Cumhur; Koc, Melih; Ozcan, Birgul
    A novel thermophilic, Gram-stain-positive, facultatively anaerobic, endospore-forming, motile, rod-shaped bacterium, strain C161ab(T), was isolated from a soil sample collected near Kizildere, Saraykoy-Buharkent power plant in Denizli. The isolate could grow at temperatures between 35 and 70 degrees C (optimum 55 degrees C), at pH 6.5-9.0 (optimum pH 8.0-8.5) and with 0-2.5% NaCl (optimum 0.5 %, w/v). The strain formed cream-coloured, circular colonies and tolerated up to 70 mM boron. Its DNA G+C content was 37.8 mol%. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. Strain C161ab(T) contained menaquinones MK-7 (96%) and MK-6 (4%). The major cellular fatty acids were iso-branched fatty acids: iso-C-15:0 (52.2%) and iso-C-17:0 (28.0%) with small amounts of C-16:0 (7.4%). Phylogenetic analysis based on the 16S rRNA gene revealed 94.6-96.8 % sequence similarity with all recognized species of the genus Anoxybacillus. Strain C161ab(T) showed the greatest sequence similarity to Anoxybacillus rupiensis DSM 17127(T) and Anoxybacillus voinovskiensis DSM 17075(T), both had 96.8% similarity to strain C161ab(T), as well as to Anoxybacillus caldiproteolyticus DSM 15730(T) (96.6%). DNA-DNA hybridization revealed low levels of relatedness with the closest relatives of strain C161ab(T), A. rupiensis (21.2%) and A. voinovskiensis (16.5%). On the basis of the results obtained from phenotypic, chemotaxonomic, genomic fingerprinting, phylogenetic and hybridization analyses, the isolate is proposed to represent a novel species, Anoxybacillus calidus sp. nov. (type strain C161ab(T)=DSM 25520(T)=NCIMB 14851(T)).
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    Thermolongibacillus altinsuensis gen. nov., sp nov and Thermolongibacillus kozakliensis sp nov., aerobic, thermophilic, long bacilli isolated from hot springs
    (Microbiology Soc, 2014) Cihan, Arzu Coleri; Koc, Melih; Ozcan, Birgul; Tekin, Nilgun; Cokmus, Cumhur
    Two novel endospore-forming, aerobic bacilli, strains E173a(T) and E265(T), were isolated from soil and sediment samples from Kozakli and Altinsu hot springs, Nevsehir (Turkey). Their young cells in the exponential phase of growth were motile, Gram-stain-positive, straight rods, 0.6-1.1x3.0-8.0 mu m in size, but they became strikingly long, approximately 0.6-1.2 by 9.0-35.0 mu m, after the stationary phase of growth. Cells varied in tests for oxidase, and had a weakly positive reaction for catalase. Both strains could grow between 40 and 70 degrees C, with optimal growth at 60 degrees C (E173a(T)) and 55 degrees C (E265(T)). Growth occurred within the range pH 5.0-11.0 with optimal growth at pH 9.0 (E173a(T)) and pH 8.5 (E265(T)). Strain E173a(T) grew within a salinity range from 0 to 1.5 % (w/v) NaCl with optimal growth at 0.5%, while strain E265(T) grew within the range 0-5.0% (w/v), with an optimum at 3.0%. The new isolates differed from each other in some phenotypic and chemotaxonomic characters as well as repetitive extragenic palindromic element PCR (rep-PCR) fingerprints. 16S rRNA gene sequence similarities suggested distant relationships with other members of the family Bacillaceae (<95.8%), although the two strains showed 97.5% sequence similarity between them, and had 55% relatedness by DNA-DNA hybridization. The DNA G+C contents were 44.8 (E173a(T)) and 43.5 mol% (E265(T)). Moreover, the chemotaxonomic data of E173a(T) and E265(T) [presence of low amounts of meso-diaminopimelic acid, A1 gamma to A1 gamma' cross-linkage types in peptidoglycan, fatty acids including iso-C-15:0 (>60%), iso-C-17:0 and C-16:0] supported the consideration of these isolates as members of a novel genus. Based upon phenotypic, phylogenetic and chemotaxonomic characteristics, it is proposed that new isolates represent a novel genus, Thermolongibacillus gen. nov., with two novel species: Thermolongibacillus altinsuensis sp. nov. (type strain E265(T)=DSM 24979(T)=NCIMB 14850(T)) and Thermolongibacillus kozakliensis sp. nov. (type strain E173a(T)=DSM 24978(T)=NCIMB 14849(T)).