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    Estimation of periostin as a biomarker for early pregnancy diagnosis in goats: a preliminary study
    (Shiraz Univ, 2021) Kose, A. M.; Saribay, M. K.; Urer, Koldas E.; Naseer, Z.; Dogruer, G.; Karaca, F.; Cetin, Coskun N.
    Background: Periostin (POSTN) is an extracellular matrix (ECM) protein that plays an important role in the metastatic process and cancer cell migration. As implantation is a similar mechanism to metastasis, it has been hypothesized that POSTN may also play a role in the implantation process. Aims: The aim of the present study was to compare POSTN and progesterone levels during the early pregnancy stage in Damascus goats. Methods: Forty goats were synchronized using progesterone based sponges and were mated upon estrus signs display. While ten goats were kept as control (CON) and were not allowed to mate. Blood samples were taken through jugular venepuncture from CON and synchronized goats on day 13, 15, 17, 19, and 21 of breeding. Progesterone and POSTN levels were determined by enzyme-linked immunosorbent assay (ELISA). Later the pregnancy diagnosis was confirmed by transabdominal ultrasonography on day 50 after mating. Results: Progesterone level was influenced by status of pregnancy and day of observation with an interaction between the status of pregnancy and day of observation in goats. Whereas POSTN level was only affected by the day of observation. Conclusion: POSTN level did not vary with progesterone level during phase of embryonic implantation in goats; however, standardization and application of different procedures for POSTN assay in a large group of animals might be useful as an early pregnancy biomarker in goats.
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    Evaluation of blood omega-3 and omega-6 levels in healthy female dogs and female dogs with mammary tumours
    (Hellenic Veterinary Medical Soc, 2021) Tuzlu, T.; Saribay, M. K.; Urer, E. Koldas; Kose, A. M.; Gozer, A.; Yakan, A.; Ozsoy, S. Y.
    The study was designed to determine the blood levels of omega fatty acids, which have an important role in the etiology of mammary tumours, in healthy and female dogs with mammary tumour. The study was carried out in 9 female dogs with histologically confirmed mammary tumour and a control group with 9 healthy female dogs without clinical mammary neoplasia. 10 ml cephalic blood samples were collected by using a 21G x 1.5.. blood collecting needle into anticoagulated tubes before the surgical removing of the mammary masses. Mastectomy was performed in all female dogs with mammary tumours and all the mammary specimens were sent to laboratory for histopathological examination. According to histopathological diagnosis results, all of the tumours were found to be malignant. Omega-3 levels were found to be higher in healthy female dogs (p<0.001) whereas omega-6 levels were higher in female dogs with mammary tumour (p<0.001). These observations support the notion tha thigh levels of omega-3 fatty acids might proove to have a protective role on mammary tumor formation in female dogs, while increased levels of omega-6 fatty acids may be related to an increased mammary tumor risk. This difference between omega-3 and omega-6 levels was found to be caused mainly by Eicosatrienoic acid. It is concluded that omega fatty acids mayplay an important role in the biological mechanism of mammary tumour in female dogs.