Yazar "Kutlu, T." seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim
    Öğe
    Anti-Müllerian Hormone Expression in the Ovarian Follicles and Factors Related to Serum Anti-Müllerian Hormone Concentrations in the Domestic Queens
    (Hellenic Veterinary Medical Soc, 2024) Gozer, A.; Dogruer, G.; Gokcek, I; Kutlu, T.; Bahan, O.; Yagci, I. Pir
    Anti-M & uuml;llerian Hormone (AMH) is a glycoprotein with 140 kDa molecular weight secreted by the granulosa cells, playing a crucial role in folliculogenesis. The present study aimed to reveal (1) the relationship between age, body weight, estrouscycle, and ovariohysterectomy with the serum AMH concentrations and (2) expression of the AMH in the ovarian follicles. Serum AMH concentrations were determined by a commercial ELISA. Ovarian expression of the AMH in the granulosa cells was detected by immunohistochemistry. Twenty femalecats were allocated to three groups according to the period of the sexual cycle, age, and body weight. The phaseof their estrouscycle was determined according to serum estradiol 17 beta and progesterone levels. Blood samples were collected to detect serum AMH concentration on Day 0- preoperative, Day 3, and Day 10 -postoperative. There was a decrease in AMH concentration on Day 3 compared to the preoperative level (Day 0) (P<0.001).No statistical difference in AMH concentration wasfound between Day 3 and 10 postoperative (P>0.05). Furthermore, the period of the sexual cycle, age, and body weight did not affect AMH levels (P>0.05). In immunohistochemical analyses,a mild AMH expression was first observed in the granulosa cells of the primary follicles, and moderate AMH expression was observed in the granulosa cells of the secondary follicles. Strong AMH expression was observed in the cumulus cells and mild AMH expression in the mural granulosa cells of the antral follicles. In conclusion, ovariohysterectomy caused a definite decline in serum AMH concentration, and AMH expression was found in all the ovarian follicles except for primordial follicles in domestic cats.
  • Küçük Resim
    Öğe
    The Effect of Alpha Lipoic Acid on Pathogenesis of Experimental Nephrolithiasis and Epithelial Mesenchymal Transition
    (Hellenic Veterinary Medical Soc, 2023) Kutlu, T.; Kazak, F.; Uyar, A.
    In the present study, it was aimed to examine the effects of early calcium oxalate (CaOx) crystal formation and deposition on the kidneys and the effects of alpha-lipoic acid (ALA) in the prevention and early treatment of CaOx deposition in rats. Sixty rats were divided into six groups (n=10 per group). Ethylene glycol (EG) and ammonium chloride (AC) (0.75% EG + 0.75% AC) were added to the drinking water of different groups for 7 or 14 days to induce nephrolithiasis. The effects of the CaOx and ALA (100 mg/kg per day orally) on kidney were investigated via histopathological, immunohistochemical, and biochemical methods. EG+AC application for both 7 and 14 days caused crystal accumulation in the tubule lumens, cystically dilated tubules, and hydropic degeneration in the tubular epithelium. However, inflammatory cell infiltration was observed merely in 14 days. When EG+AC administration was applied for 14 days only, it caused expression of ED1, alpha smooth muscle actin (alpha-SMA), and vimentin in the tubulointerstitial areas. However, alpha-SMA and vimentin expression was not observed in tubular epithelial cells. Transforming growth factor beta-1 (TGF-01) expression was also detected in the tubules and intertubular cells at 14 days. It was determined that ALA administration with EG+AC reduced the crystal accumulation in the tubule lumens (p<0.001), the degeneration of the tubular epithelium (p<0.001), and the expression of TGF-01. In addition, it was de- tected that ALA caused an increase in glutathione peroxidase (GPx) (p<0.001) and Catalase (CAT) (p>0.05) activities, which decreased with EG+AC application. This study suggests that ALA may be an effective strategy for reducing acute kidney injury caused by CaOx.
  • Küçük Resim
    Öğe
    The effects of black cumin (Nigella sativa l..) seed on carcass characteristics, kidney oxidant antioxidant levels and ileum histomorphology in Japanese quails
    (Hellenic Veterinary Medical Soc, 2023) Kazak, F.; Cimrin, T.; Alasahan, S.; Kisacam, M. A.; Kutlu, T.
    This study aims to determine the effects of supplementation of different level of black cumin seeds (NS) to quail feeds on carcass characteristics, kidney oxidant antioxidant levels, and ileum histomorphology. A total of 432 mixed-gender three days old Japanese quailswere randomly divided into four groups, including 108 animals each and further into 6 replications consisting of 18 chicks. The groups were fed on either a basal diet alone (Control Group) or a basal diet supplemented with NS at rates of 0.5% (NS-0.5 Group), 1% (NS-1 Group), and 2% (NS-2 Group).It was determined that the back+neck weight in the NS-2 group and head weight and ratio in the NS-1 and NS-2 groups decreased. The thigh rate increased in the NS-0.5 group compared to the control and other NS groups. While the wing weight increased in male quails fed with NS-supplemented feed in NS-1 and NS-2 groups compared to the control group, the abdominal fat weight decreased in NS-supplemented groups.Also, the slaughter weight and hot carcass weight in female quails were significantly reduced in the NS-2 group compared to the control group.Kidney glutathione (GSH), glutathione peroxidase (GPx), and male quails' catalase (CAT) values were increased in NS groups compared to the control group. The kidney vitamin C (Vit C) increased only in the NS-2 group compared to the con-trol. In the NS-2 group, the villus height decreased both on a group basis and in female quails, while the villus width decreased only in female quails. Consequently, it was determined that dietary supplementation of NS at rates of 0.5% increased the thigh rate, dietary supplementation at three different level of NS were effective in maintaining the kidney oxidant-antioxidant balance, and the ileum histomorphology did not change except for dietary supplementation of NS at rates of 2%. Therefore, the result of this study can be stated that NS can be used as a natural antioxidant source in the quail diet.