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Öğe Determination of chemical component of essential oil of Origanum dubium plants grown at different altitudes and antifungal activity against Sclerotinia sclerotiorum(Wiley, 2022) Turkmen, Musa; Kara, Merve; Maral, Hasan; Soylu, SonerThe aim of this study was to determine effect of altitude on the yields, composition, and antifungal activities of the essential oil isolated from Origanum dubium plant populations collected at four different altitudes. The essential oil yield, the content of four compounds and antifungal activities against Sclerotinia sclerotiorum were found to vary in relation to the different altitudes examined. According to the GC-MS analysis, carvacrol (46.51%-85.65%), linalool (0.24-48.37), p-cymene (1.89%-7.89%), and gamma-terpinene (0.28%-6.31%) were determined as major compounds of essential oils of the plants collected from different altitudes. Altitude seems one of the factors that affected the quantity and quality of the essential oil of O. dubium. A significant correlation between the amount of carvacrol, p-cymene, and antifungal activities was determined. The essential oil of plant having the highest content of carvacrol (85.65%) showed the strongest antifungal activity against S. sclerotiorum at relatively very low concentration used (0.75 mu l petri(-1)). Practical applications The essential oil yields, the chemical compositions and antifungal activities against Sclerotinia sclerotiorum were found to vary in relation to the altitudes where plants grown. Since the yield, chemical composition, and biological activities was affected, altitude seems to be an important factor that should be considered. To the best of our knowledge, this is the first study reporting antifungal activities of essential oil of Origanum dubium against devastating plant fungal disease agent S. sclerotiorum.Öğe Determination of essential oil constituents and antioxidant activities of hybrid species Salvia x karamanensis Celep & B. T. Drew and its parents (Salvia aucheri Benth. subsp. canescens (Boiss. & Heldr.) Celep, Kahraman & Dogan x Salvia heldreichiana Boiss. ex Benth.)(Pergamon-Elsevier Science Ltd, 2024) Maral, Hasan; Cecen, Omer; Baydir, Ayseguel Turk; Turkmen, MusaSalvia x karamanensis is endemic to Turkiye and is a hybrid of Salvia aucheri subsp. canescens and Salvia heldreichiana. In this study, the essential oil content and composition of S. x karamanensis hybrid species and its parents S. aucheri subsp. canescens, S. heldreichiana were determined. Essential oils of the plants were obtained by hydro distillation method using Clevenger apparatus for 3 h and the essential oil components were determined by GC -MS. As a result of the distillation, it was determined that S. aucheri subsp. canescens, contains 0.80%, while S. x karamanensis and S. heldreichiana contain 0.64 and 0.48% essential oil, respectively. The major components of the essential oils were borneol, 1,8-cineole, camphor, alpha-pinene, borneol and alpha-terpineol. The DPPH antiradical activity of the samples was determined as 46.70 +/- 0.09, 33.78 +/- 0.68 and 25.93 +/- 0.72 (S. aucheri subsp. canescens, S. x karamanensis and S. heldreichiana), respectively. The highest phenolic content in terms of gallic acid was obtained from S. aucheri subsp. canescens with 15,02 mg/100 ml, followed by S. x karamanensis with 14,43 mg/100 ml and S. heldreichiana with 13,43 mg/100 ml.Öğe The effect of different drying temperatures on the essential oil content and chemical composition of Lavandula angustifolia Mill.(2024) Türkmen, Musa; Eren, Yılmaz; Maral, Hasan; Kaya, Durmuş AlpaslanDrying temperatures affect the content and composition of essential oils in plants containing essential oils due to the organs where essential oils are synthesized and stored. For this reason, many studies have been carried out to determine the appropriate drying temperature to obtain the highest amount and the best quality essential oil. In present study, it is aimed to determine the effects of different drying temperatures on essential oil content and components in lavender (Lavandula angustufolia Mill.). The samples dried at four different temperatures (35°C, 45°C, 55°C and 65°C) were isolated for 3 hours using Clevenger type apparatus and the obtained oils were analyzed by GC-MS. The essential oil contents obtained at 25°C, 35°C, 45°C, 55°C, and 65°C were 1.17%, 0.96%, 0.94%, 0.65%, and 0.18% respectively. It was determined that the major components of essential oils obtained at different drying temperatures were 1.8-cineole (17.88-50.15%), camphor (32.60-48.86) and borneol (3.46-9.45%). The highest 1,8-cineole ratio was found in samples dried at 55°C (50.15%) but the lowest in samples dried at 65°C (17.88%). The highest and lowest camphor ratios were determined in samples dried at 65°C (48.86%) and samples dried at 55°C (32.60%) recpectively. The highest (9.45%) borneol ratio was obtained in samples dried at 65°C, while the lowest (3.46%) ratio was obtained in samples dried at 55°C. The results obtained in the present study showed that Lavender essential oil content and composition were affected by drying temperatures and the optimum drying temperature was 35 oC.Öğe Essential oil composition of Nepeta isaurica Boiss. Heldr. apud Bentham and Nepeta sulfuriflora P.H. Davis growing naturally in Türkiye(2023) Maral, Hasan; Çeçen, Ömer; Türkmen, MusaNepeta species are widely used in folk medicine as a diaphoretic, diuretic, antiseptic, astringent, anti-asthmatic, cough suppressant, antispasmodic, antipyretic, sedative and stomachic. This study was designed to examine the chemical composition of the essential oil of Nepeta isaurica and Nepeta sulfuriflora. It was determined that the aerial parts of the N. isaurica plant contained 0.72% essential oil, 37 compounds were identified representing 98.86% of the total oil analyzed via GC-MS. The major components of the essential oil obtained from these plants were trans-pinocarveol, ?-pinene, linalool, 1,8-cineole, ?-terpineol, ?-cadinol, spathulenol and ?-pinene. It was determined that the aerial parts of N. sulfuriflora contained 0.64% essential oil. GC-MS analysis identified 37 compounds representing 99.07% of the total oil. It has been determined that the main component of the essential oil in N. sulfuriflora is 1,8-cineol (72.05%). As a result of the study, it was concluded that N. sulfuriflora could be used as a 1,8-cineol reservoir.
