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    Prevalence and antimicrobial resistance of Salmonella isolated from bovine and ovine samples in slaughterhouses of Algiers, Algeria
    (Hellenic Veterinary Medical Soc, 2018) Nouichi, S.; Ouatouat, R.; Can, H. Y.; Mezali, L.; Belkader, C.; Ouar-Korichi, M.; Bertrand, S.
    The aims of our work are to estimate the prevalence of Salmonella isolated from carcasses and feces of cattle and sheep in the two biggest slaughterhouses in Algiers, Algeria, and to characterize the obtained strains by serotyping and antimicrobial resistance testing. The detection of Salmonella was performed by the conventional culture method and isolates were confirmed by PCR. Susceptibility to antibiotics was carried out by agar disc diffusion method. The results showed that 10.17% of samples were Salmonella positive. Carcass samples were more contaminated than fecal samples. Serotyping of the 84 Salmonella isolates has enabled to identify 10 different serovars; the most predominant was S. Muenster. The invA gene was detected in 96.43% of isolates whereas all S. Typhimurium strains were positive for spy gene. Sixty-eight (80.95%) isolates were resistant to at least one of the 28 antibiotics tested and exhibited 17 different antimicrobial resistance patterns. The most frequently observed resistance was to streptomycin (69.05%). While 22.62 % of the isolates were MDR, two S. Typhimurium showed an ACSSuT'' pentaresistance pattern. Considering the importance of this group of bacteria for public health, Salmonella control is necessary at several steps of food production to ensure safe products for consumers.
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    Prevalence and distribution of staphylococcal enterotoxin genes among Staphylococcus aureus isolates from chicken and turkey carcasses in Algeria
    (Hellenic Veterinary Medical Soc, 2018) Mebkhout, F.; Mezali, L.; Hamdi, T. M.; Cantekin, Z.; Ergun, Y.; Ramdani-Bouguessa, N.; Butaye, P.
    This study is aimed to determine the prevalence of staphylococcus aureus (S.aureus) by biochemical tests in poultry carcasses. It is also intend to detect the distribution of genes for classical staphylococcal enterotoxins A, B, C, D and E (sea, seb, sec, sed and see) and for gene femA, specific for S. aureus species, using multiplex PCR. A total of 385 samples of neck skins from fresh poultry carcasses were collected during the period 2012-2013 from 16 different slaughterhouses located in the region of Algiers, Algeria. The overall prevalence of S.aureus in freshly slaughtered poultry carcasses was 41.56%, with an individual prevalence of 40.63% and 45.71% for chicken and turkey respectively. From the 95 strains of S.aureus identified by biochemical tests. 82 (86.32%) isolates were femA positive using multiplex PCR. The investigation has also revealed the presence of both enterotoxins B and D, with a predominance of seb (13.33%) followed by sed (1.67%), in the chicken carcasses while in turkey only sed was detected (4.55%) It has been found that strains of S.aureus of poultry origin can be enterotoxigenic with the predominance of genes encoding for enterotoxins seb in chicken and sed in turkey. As enterotoxins can be produced in adequate amounts to induce foodborne illnesses, these potential dangers must be considered in terms of a real risk to public health.
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    Prevalence and Distribution of Staphylococcal Enterotoxin Genes Among Staphylococcus Aureus Isolates From Chicken and Turkey Carcasses in Algeria
    (Hellenic Veterinary Medical Society, 2018) Mebkhout, F.; Mezali, L.; Hamdi, T.M.; Cantekin, Z.; Ergun, Y.; Ramdani-Bouguessa, N.; Butaye, P.
    This study is aimed to determine the prevalence of staphylococcus aureus (S.aureus) by biochemical tests in poultry carcasses. It is also intend to detect the distribution of genes for classical staphylococcal enterotoxins A, B, C, D and E (sea, seb, sec, sed and see) and for gene femA, specific for S.aureus species, using multiplex PCR. A total of 385 samples of neck skins from fresh poultry carcasses were collected during the period 2012-2013 from 16 different slaughterhouses located in the region of Algiers, Algeria. The overall prevalence of S.aureus in freshly slaughtered poultry carcasses was 41.56%, with an individual prevalence of 40.63% and 45.71% for chicken and turkey respectively. From the 95 strains of S.aureus identified by biochemical tests, 82 (86.32%) isolates were femA positive using multiplex PCR. The investigation has also revealed the presence of both enterotoxins B and D, with a predominance of seb (13.33%) followed by sed (1.67%), in the chicken carcasses while in turkey only sed was detected (4.55%) It has been found that strains of S.aureus of poultry origin can be enterotoxigenic with the predominance of genes encoding for enterotoxins seb in chicken and sed in turkey. As enterotoxins can be produced in adequate amounts to induce foodborne illnesses, these potential dangers must be considered in terms of a real risk to public health. Copyright © 2019 F. Mebkhout, L. Mezali, T.M. Hamdi, Z. Cantekin, Y. Ergun, N. Ramdani-Bouguessa, P. Butaye