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Öğe Methicillin Resistance in Staphylococcus pseudintermedius Isolated from Shelter Dogs in Turkey(Kafkas Univ, Veteriner Fakultesi Dergisi, 2014) Sareyyupoglu, Baris; Mustak, H. Kaan; Cantekin, Zafer; Diker, K. SerdarStaphylococcus pseudintermedius is a recently described species of staphylococci that is frequently isolated from dogs. The increase in antimicrobial resistance in staphylococci and transfer of S. pseudintermedius from infected pets to humans threaten the public health worldwide. In this study, it was aimed to determine the prevalence of S. pseudintermedius from skin infections of dogs and to determine the prevalence of methicillin-resistance in S. pseudintermedius isolates by phenotypic and genotypic methods. A total of 41 staphylococci were isolated from 53 dogs. Thirty three (80.4%) staphylococci were identified as S. pseudintermedius by PCR-RFLP analysis of pta gene. Methicillin resistance was also identified in 11 (33.3%) of these isolates by inoculation on chromogenic chromID MRSA agar, oxacillin disc diffusion method and the determination of mecA gene by PCR. This is the sole report in Turkey that described the situation of methicillin resistant S. pseudintermedius.