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Öğe Characterization of the partial VP2 gene region of canine parvoviruses in domestic cats from Turkey(Springer, 2012) Muz, Dilek; Oguzoglu, T. Cigdem; Timurkan, M. Ozkan; Akin, HumayCanine parvoviruses (CPVs) is a category comprising three closely related viruses, CPV, feline panleukopenia virus (FPLV), and mink enteritis virus, all of which cause serious diseases, especially in young cats. In this study, molecular detection and genetic analysis of a partial VP2 gene region of CPVs from domestic cats living in Turkey between 2006 and 2010 was performed by PCR amplification and sequence analysis. The results indicated that CPV-2a, CPV-2c, and FPLV were circulating in vaccinated and unvaccinated cats. This is the first description of molecular characterization of CPVs in domestic cats from Turkey.Öğe First molecular characterization of feline immunodeficiency virus in Turkey(Springer Wien, 2010) Oguzoglu, Tuba Cigdem; Timurkan, Mehmet Ozkan; Muz, Dilek; Kudu, Aysegul; Numanbayraktaroglu, Basak; Sadak, Seda; Burgu, IbrahimIn this study, strains of feline immunodeficiency virus (Fly), designated TR-D, TR-Mo and TR-Mi, isolated from three cats in Turkey, were characterized. PCR products (859 bp) from the envelope (env) gene region were amplified and sequenced, and possible geographical differences in the env gene region of Turkish FIV strains are discussed. Phylogenetic analysis of two strains showed that FIV subtype B was present in Turkey. Phylogenetic analysis showed that one new Turkish FIV strain occupies a separate branch from known clusters (subtypes A to E) from the USA, Canada, Europe and Japan.Öğe First molecular characterization of visna/maedi viruses from naturally infected sheep in Turkey(Springer Wien, 2013) Muz, Dilek; Oguzoglu, Tuba Cigdem; Rosati, Sergio; Reina, Ramses; Bertolotti, Luigi; Burgu, IbrahimRecent worldwide serological and genetic studies of small ruminant lentiviruses (SRLV) have led to the description of new genotypes and the development of new diagnostic tests. This study investigated the detection and molecular characterization of visna/maedi virus (VMV) infection in serum and blood samples from pure and mixed sheep breeds acquired from different regions in Turkey using ELISA and PCR techniques. The prevalence of VMV was 67.8 % by ELISA and/or LTR-PCR with both assays showing a medium level of agreement (kappa: 0.26; +/- A 0.038 CI). Positivity of VMV in sheep increased according to the age of the animal, although PCR positivity was higher than ELISA in young individuals. Phylogenetic analysis of 33 LTR sequences identified two distinct clades that were closely related to American and Greek LTR sequences. Phylogenetic analysis of 10 partial gag gene sequences identified A2, A3, A5, A9, A11 subtypes of genotype A SRLVs. In vitro culture of all isolates in fetal sheep lung cells (FSLC) showed a slow/low phenotype causing less or no lytic infection compared with infection with the WLC-1 American strain characterized by a rapid/highly lytic phenotype. Phylogenetic analysis revealed that Turkish VMV sequences preceded the establishment of American or Greek strains that were associated with the migration of sheep from the Middle East to Western Europe several centuries ago. This is the first study that describes Turkish VMV sequences with the molecular characterization of LTR and gag genes, and it strongly suggests that SRLV-genotype A originated in Turkey.Öğe Molecular characterization of Bovine virus diarrhea viruses species 2 (BVDV-2) from cattle in Turkey(Springer, 2010) Oguzoglu, Tuba Cigdem; Muz, Dilek; Yilmaz, Volkan; Alkan, Feray; Akca, Yilmaz; Burgu, IbrahimFive BVDV species 2 (BVDV-2) isolates were detected from cattle in Turkey. Phylogenetic analysis was performed based on the 5' untranslated regions (UTRs) and E2 coding gene regions, respectively. The isolates were closely related to BVDV-2a strains from North America and Canada used as references. This is the first report of the detection of BVDV-2 in naturally infected Turkish cattle. It is important to consider BVDV-2 for planning future BVDV control and vaccination programs in Turkey.Öğe Molecular detection of Nosema ceranae and Nosema apis infections in Turkish apiaries with collapsed colonies(Int Bee Research Assoc, 2010) Muz, Mustafa Necati; Girisgin, Ahmet Onur; Muz, Dilek; Aydin, Levent[Abstract Not Available]Öğe The molecular prevalence of Borrelia burgdorferi, Babesia spp., and Anaplasma spp. in shelter dogs of the Thrace Region in Turkey(Tubitak Scientific & Technological Research Council Turkey, 2022) Altug, Nuri; Muz, Mustafa Necati; Muz, Dilek; Yipel, Fulya AltinokThe study aimed to update the molecular prevalence of some tick-borne pathogens (Borrelia burgdorferi, Babesia spp., and Anaplasma spp.) in the shelter dogs of the Thrace Region, Turkey. The study was carried out on 450 dogs from 7 pet shelters. The individual data of the dogs were recorded, and blood samples were collected in tubes with anticoagulants (EDTA). Then, individual PCR protocols were applied to all samples for the three infective agents. PCR test results recorded for B. burgdorferi is 38.22% (n = 172), 24.22% (n = 109) for Babesia spp., and 21.6% (n = 97) for Anaplasma spp. The positivity of dogs with at least one pathogen was 56.22% (n = 253). Only one pathogen positivity rate was determined in positive samples as 56.92% (n = 144). The positivity was determined 33.99% (n = 86) for two pathogens and 9.09% (n = 23) for three pathogens. The coexistence of the two pathogens was statistically significant (p < 0.01). The effect of sex and age was not statistically significant in the agent positivity (p < 0.01, p < 0.05). Among tested three pathogens, only the positivity of B. burgdorferi (p = 0.155) was statistically significant compared with the prevalence of the others (p < 0.01). As a result, pathogens transmitted by ticks in shelter dogs of the Thrace region were simultaneously investigated and detected for the first time. Results revealed that shelter dogs pose a hidden risk for animal and human health in the region and so the necessity to plan systematic epidemiological studies about tick-borne zoonose pathogens more frequently.Öğe Prevalence of feline coronavirus (FCoV) and feline leukemia virus (FeLV) in Turkish cats(2010) Tuba Çiğdem Oguzoglu; Can Sahna, Kezban; Ataseven, Veysel Soydal; Muz, DilekThe aim of this study was to determine prevalence of Feline Coronavirus (FCoV) with concurrent Feline Leukemia Virus (FeLV) infections in that had no clinical signs living in different cities of Turkey. FCoV antibodies and FeLV antigens/antibodies were investigated in cats by using ELISA. The results showed that 37 (69.8%) of cats had FCoV antibodies and 2 (3.8%) of cats had FeLV antigens, 4 (7.5%) of cats had FeLV antibodies. Additionally, the FCoV antibodies were detected in all cats having FeLV antigen (n=2) and 75% of cats having FeLV antibody (n=4). The results of study showed the presence both of FCoV and FeLV of cats in association with different age, sex living conditions and environment in Turkey.Öğe Survey of the occurrence of Deformed Wing Virus and multiple parasites of queens (Apis mellifera L.) in apiaries with collapsed colonies in Hatay, Turkey(Taylor & Francis Ltd, 2009) Muz, Dilek; Muz, Mustafa N.Honey bee queens in Hatay, Turkey, were investigated to reveal the status of Deformed Wing Virus (DWV) and parasites. DWV, Nosema sp., Malpighamoeba mellificae, and Varroa destructor were detected among samples. This is the first record of DWV in honey bee queens with multiple parasitic infections in apiaries with collapsed colonies in Turkey.
