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Öğe Borik asit uygulamasının sıçan böbrek ve testis dokusunda oluşturduğu hasara karşı omega-3 yağ asitlerinin koruyucu etkisinin histopatolojik olarak incelenmesi(2014) Nacar, Ahmet; Selçuk, Yasin; Okuyan, Hamza Malik; Sefil Kaplan, Nebihat; Deligönül, Erkan; Nacar, EmelAmaç: Bu çalışmada borik asitin böbrek ve testis dokuları üzerine toksik etkilerine karşı omega-3 yağ asitlerinin koruyucu etkileri araştırıldı. Yöntemler: Çalışmada 32 adet Wistar albino rat kullanı- larak 4 grup oluşturuldu. Kontrol, Omega-3 (10 gün sü- reyle 400 mg/kg/gün), Borik asit (375 mg/kg/gün, 10 gün), Borik asit+Omega-3. Böbrek ve testis dokuları belirli histopatolojik bulguların yaygınlığına göre puanlandı. Bulgular: Histopatolojik analizde, borik asit testis ve böbrekte anlamlı derecede hasar oluşturdu. En belirgin bulgular böbrekte glomerüllerde büzülme, nekroz, kanama ve tübüler hücrelerde dejenerasyon; testiste ise seminifer tübülde hücre kaybı, hücrelerin bazal laminadan kopması ve epitel hücrelerin dejenerasyonu şeklindeydi. Omega-3 uygulaması bu hasarı belirgin bir biçimde hafifletti. Sonuç: Literatür analizimize göre bu çalışma borik asitin indüklediği böbrek ve testis hasarına karşı omega-3 yağ asitlerinin koruyucu etkilerinin gösterildiği ilk çalışmadır.Öğe The effect of caffeic acid phenethyl ester on isoproterenol-induced myocardial injury in hypertensive rats(2014) İlhan, Selçuk; Yılmaz, Nigar; Nacar, Emel; Motor, Sedat; Oktar, Süleyman; Şahna, EnginObjective: The aim of this study is to investigate the effects of caffeic acid phenethyl ester (CAPE) on isoproterenol (ISO)-induced myocardial injury in hypertensive rats. Methods: Rats were divided into 4 groups (n=29): Control group (n=8), L-NNA (NG-Nitro-L-arginine) group (n=8), L-NNA+ISO (L-NNA+isoproterenol) group (n=7) and L-NNA+ISO+CAPE (L-NNA+ISO + caffeic acid phenethyl ester) group (n=6). ISO (150 mg/kg/day) was given intraperitoneally (i.p.) once a day for 2 consecutive days (at the 12th and 13th days of L-NNA treatment). NG-Nitro-L-arginine (L-NNA) was given orally (25 mg/kg/day) in drinking water for 14 days. CAPE (10 ?mol/kg/day) was given (i.p.) for 7 days after the first week. Systolic blood pressure (SBP) was evaluated by the tail-cuff method and biochemical analysis were performed using an autoanalyzer and a spectrophotometer.Results: SBP in all L-NNA-treated groups was found to be increased at seventh day. AST and LDH levels in LNNA+ISO group were significantly increased compared to control (AST: 125±5 vs. 105±2; LDH: 861±154 vs. 571±46 U/L respectively) (p<0.05). Also, ISO caused to extensive necrosis and mononuclear cell infiltration in hypertensive rat myocardium. CAPE application reversed the enhanced AST and LDH levels as well as the extensive necrosis and the mononuclear cell infiltration in LNNA+ISO+CAPE group compared LNNA+ISO.Conclusion: According to our findings, it might be suggested that CAPE may be a favorable agent to protect the hypertensive myocardium from the injury induced by isoproterenol via mechanisms such as the induction of the antioxidant enzymes and the inhibition of lipid peroxidation.Öğe Effect of Thymoquinone on Oxidative Stress in Escherichia coli-Induced Pyelonephritis in Rats(Elsevier Science Inc, 2011) Evirgen, Omer; Gokce, Ahmet; Ozturk, Oktay Hasan; Nacar, Emel; Onlen, Yusuf; Ozer, Burcin; Motor, Vicdan KoksaldiBACKGROUND: Recurrent urinary tract infections are important in children and adults with diabetes mellitus and/or incontinence due to risk of pyelonephritis (PYN) and renal damage. There is a positive correlation released free radicals during PYN and renal damage. Experimental studies showed that antioxidant agents improve renal damage when used immediately after bacterial inoculation. OBJECTIVE: The aim of the present study was to evaluate whether treatment by thymoquinone (TQ) before or during Escherichia coli inoculation prevents oxidative damage in acute pyelonephritis (PYN) in an ascending obstructive rat model. METHODS: In this study, 42 Wistar rats were grouped as follows: control, PYN (24, 48, and 72 hours), and TQ-PYN (24, 48, and 72 hours). E. coli (1 x 10(9) colony forming units) was inoculated into the bladder via urethral catheterization in both the PYN and TQ groups. TQ injections were performed 24 hours before bacteria inoculation and repeated at 24-hour intervals during the indicated time at a dose of 10 mg/kg body weight intraperitoneally in TQ groups. RESULTS: Superoxide dismutase activity was statistically lower in the TQ-PYN-48 and -72 groups than the PYN-48 and -72 groups (P < 0.001, P = 0.004, respectively). Catalase activity was significantly higher in PYN-24, -48, and -72 groups than the control group (P < 0.001). In addition, there was a significant difference between the TQ-PYN-24, -48, and -72 groups and PYN groups in terms of glutathione peroxidase activity (P < 0.001, P = 0.026, P = 0.046, respectively). When the TQ-PYN-72 group was compared with the PYN-72 group, malondialdehyde levels were significantly lower in the TQ-PYN-72 group than in the PYN-72 group (P = 0.033). A histologic examination also confirmed the protective effect of TQ. In statistical analysis of histopathologic findings, there were significant differences between the PYN-24 and TQ-PYN-24, PYN-48 and TQ-PYN-48, and PYN-72 and TQ-PYN-72 groups (P = 0.008, P < 0.001, P < 0.001, respectively). CONCLUSIONS: The results indicate that TQ administration attenuated the oxidative damage that occurred in PYN and, therefore, could be used as a supportive agent to protect the kidneys from oxidative damage caused by PYN. (Curr Ther Res Clin Exp. 2011;72:204-215) (C) 2011 Elsevier HS Journals, Inc. All rights reserved.Öğe IMMUNOHISTOCHEMICAL INVESTIGATION OF ENDOMETRIAL LEUKOCYTES IN IMPLANTATION PERIOD IN RATS WITH STREPTOZOTOSIN-INDUCED DIABETES(Vesalius Univ Medical Publ, 2016) Nacar, Emel; Bozkurt, Yesim Akaydin; Koc, Ahmet; Nacar, AhmetOur first aim was to determine the total leukocyte profile in implantation. Second aim was to detect the changes in uterine leukocyte profile in diabetes, a common accompanying disease. For this purpose 4 groups are formed with Wistar albino rats weighing 250-300 g. Two of the groups were non-diabetic and two of them were diabetic. One of the diabetic and one of the non-diabetic groups were left pregnant. Then uterus tissues of pregnant animals were removed in the 5th and 7th days of pregnancy together with tissues of other two non-pregnant groups. Tissues were analyzed immunohistochemically with antibodies CD45, CD3, CD4, CD8, CD56, CD68 and CD79a. It was revealed that pregnancy increased immune staining of CD68, CD3, CD45 and CD56 in endometrium. In addition it was observed that immune staining density of CD68, CD45 and CD56 decreased in diabetes. In the histopathological examination, significant degeneration was detected in the endometrium of diabetic rats. Diabetes could decrease leukocyte proportions in decidua in early pregnancy periods. Therefore immune cell therapies could be administrated in diabetes related problems of pregnancy.Öğe Investigation of the protective effect of erdosteine against cyclosporine-induced injury in rat liver with histological and biochemical methods(2015) Nacar, Ahmet; Karaboğa, İhsan; Okuyan, Hamza Malik; Kaplan Sefil, Nebihat; Nacar, Emel; Motor, Sedat; Akküçük, Seçkin; Özkan, Orhan VeliBackground/aim: In the present study, the protective effect of erdosteine against cyclosporine-induced injury in rat liver was investigated with histological and biochemical methods. Materials and methods: Thirty-two Wistar albino male rats were randomly divided into 4 groups: control (n = 8), cyclosporine (n = 8, 20 mg kg–1 day–1 i.p.), cyclosporine + erdosteine (n = 8, erdosteine 12 mg kg–1 day–1 orally), and erdosteine (n = 8). At the end of day 12, liver tissues were removed for histological and biochemical analysis. After liver tissues were fixed in 10% buffered neutral formalin, routine histological processes were applied and tissue sections were stained with hematoxylin and eosin, periodic acid–Schiff, and elastic fiber stain methods. One hundred lobules of liver were examined for each group and evaluated statistically. The levels of malondialdehyde and glutathione peroxidase, as well as the activities of superoxide dismutase, were determined. Results: The cyclosporine group showed significant histopathological changes compared to the control. In the cyclosporine + erdosteine group, histopathological changes of hepatic damage were markedly reduced. Histological findings were supported by biochemical results. Conclusion: Erdosteine could attenuate cyclosporine-induced liver injury.Öğe Protective Effect of Ellagic Acid on Paraquat-induced Kidney Hazards in Rats(Iranian Soc Nephrolgy, 2017) Silfeler, Ibrahim; Alp, Harun; Dorum, Bayram Ali; Nacar, Emel; Arslan, Selda; Uygur, VeliIntroduction. Paraquat is a commonly used highly toxic herbicide. Despite many studies on detoxification of paraquat, an efficient and safe antidote has not been introduced for toxic cases in human being. The aim of this study was to investigate the effect of ellagic acid (EA) on paraquat-induced kidney hazards in rats. Materials and Methods. Sixty rats were randomly assigned as controls and 5 treatment groups (n = 10 each) receiving EA only, paraquat at doses of 15 mg/kg and 45 mg/kg, and paraquat at the same doses plus EA. Paraquat was intraperitoneally injected and the EA was orally given. Kidney tissues were stained with hematoxylin-eosin for histopathologic investigation. Results. Pathologic scoring showed that paraquat at the higher dose was associated with higher scores than the in the controls, EA group, and the high-dose paraquat group with EA treatment (P < .001 for all comparisons). It was noted that paraquat caused a serious damage in the kidney and the EA treatment significantly reduced the extent of the damage. Conclusions. This study showed the protective effects of EA against paraquat-induced nephrotoxicity histologically. Ellagic acid provided significant improvement in glomerular and tubular structure.Öğe Streptozotosin ile diyabet oluşturulan sıçanlarda implantasyon döneminde endometriyumdaki immün sistem hücrelerinin immünhistokimyasal yöntemlerle incelenmesi(Hatay Mustafa Kemal Üniversitesi, 2012) Nacar, Emel; Koç, Ahmetİmplantasyonun başarıyla gerçekleştirilebilmesindeki en kritik olay dişi vücudunun zigota gösterdiği immün toleranstır. İmplantasyon ile başlayarak gebeliğin sonuna dek uterustaki immün hücrelerin oranları ve işlevlerinde çeşitli değişimler gerçekleşmektedir. Dolayısıyla implantasyon mekanizmasının tam olarak anlaşılabilmesinin bir yolu uterustaki immün hücre dağılımının implantasyon sırasında gösterdiği değişiklikleri incelemektir. İmplantasyonun daha iyi anlaşılmasını kolaylaştıracak bir diğer yol ise patolojik durumlarda implantasyonun nasıl etkilendiğini araştırmaktır. Bu nedenle tezde iki amaç hedeflenmiştir. Birinci amaç çeşitli çalışmalarda farklı yönleriyle ele alınan uterusta implantasyon sırasındaki lökosit profilini bir bütün olarak değerlendirmektir. İkinci amaç ise uterustaki hücresel profilin diyabet gibi gebelikte sık görülen bir hastalık sonucu nasıl değiştiğini saptamaktır. Bu amaçla 250?300 g ağırlığında, erişkin dişi Wistar Albino sıçanlardan ikisi normal ikisi diyabetik olacak şekilde 4 grup oluşturuldu. Diyabetik ve normal gruplardan birer tanesi gebe bırakıldı. Gebe olan hayvanlardan gebeliklerinin 5 ve 7. günlerinde uterus dokuları alınarak diğer gruplarla endometriyumlarındaki CD45, CD3, CD4, CD8, CD56, CD68 ve CD79a tutulumları açısından immünhistokimyasal yöntemlerle inceleme yapıldı. Çalışmanın sonucunda gebeliğin endometriyumda CD68, CD3, CD45 ve CD56 tutulumunu arttırdığı görüldü. Diyabetin etkisine bakıldığında ise CD68, CD45 ve CD56 tutulumunun diyabetik gruplarda daha zayıf olduğu izlendi. Histopatolojik değerlendirmede diyabet olan gruplarda endometriyumda belirgin dejenerasyon saptandı.Sonuç olarak normalde implantasyon döneminde en yüksek popülasyona sahip olan uNK ve makrofaj oranının diyabetle birlikte azaldığı görüldü. Bu azalmanın, diyabetin uterus üzerindeki olumsuz etkilerini arttırabileceği düşünüldü. Öte yandan daha uzun süre izlem yapılmasının da diyabetin ileri dönem etkilerinin hücresel düzeydeki yansımalarının anlaşılmasında daha verimli olabileceği kanısına varıldı.
