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  • Küçük Resim
    Öğe
    The investigation of the presence of bovine herpesvirus type 4 (BoHV-4) in cows with metritis in a dairy herd
    (2010) Bilge Dağalp, Seval; Güngör, Elvin; Demir, Ayşe Başak; Muz, Dilek Pınar; Yılmaz, Volkan; Oğuzoğlu, Tuba Çiğdem; Ataseven, Veysel Soydal; Alkan, Feray
    To investigate the occurrence of bovine herpesvirus type 4 (BoHV-4) infection in a dairy herd including cows with metritis, various methods were used for the diagnosis BoHV-4 in vaginal discharge samples. Blood serum samples were obtained from 368 cows for serodiagnosis of BoHV-4 infection. Of 368 serum samples, 256 (69.6%) were found positive for BoHV- 4 specific antibodies. For the virological study, vaginal discharge samples of 55 cows with metritis were used and tested for BoHV-4 using polymerase chain reaction (PCR), virus isolation and immunofluorescence techniques. Twenty-nine percent (16/55) of the vaginal discharge samples obtained from cows with metritis were found positive for BoHV-4 DNA by PCR. In this study, the presence of BoHV-4 was determined in cows with metritis in post-partum period in a dairy herd.
  • Küçük Resim
    Öğe
    Porcine circovirus type 2 infection in Turkey
    (2011) Karaoğlu, Taner; Oğuzoğlu, Tuba Çiğdem; Ataseven, Veysel Soydal; Tan, Mehmet Tolga; Alkan, Feray
    Porcine circovirus is the smallest known DNA virus and is identified and characterized by two types: PCV type 1 (PCV 1) and PCV type 2 (PCV 2). Porcine circovirus type 1 (PCV 1) was first identified in 1974 and was recognized as a nondisease- causing agent that frequently occured in laboratory tissue cultures. As for PCV 2, it is defined as an antigenically and genomically different PCV which was commonly seen in swine populations and led to clinic disorders at the end of the 1990s. PCV2 infection in pigs can cause a wide variety of clinical signs and syndromes. This study aims to analyze the presence of PCV 2 related to different clinic cases and syndromes found on some pig farms. To this end, 86 nasal swab samples from two different pig farms and 12 lung tissue pieces from wild boars were collected to detect whether PCV 2 was present. In the study, 98 samples were used in total. The one step PCR technique was applied to the samples obtained. The samples were analyzed in terms of PCV presence; 38 of the samples were identified as PCV positive. Thirty-one of the 38 samples which were identified as PCV positive were identified as PCV 2 in the discriminant diagnosis, while 7 were evaluated as PCV 1.
  • Küçük Resim
    Öğe
    Türkiye'de abortla ilişkili equid herpesvirus tip 1 izolatlarının gB geninin kısmi sekansı
    (2016) Ataseven, Veysel Soydal; Oğuzoğlu, Tuba Çiğdem; Dinçer, Ender; Seval, Bilge Dağalp
    Equid herpesvirus 1 (EHV-1), kısraklarda sporadik veya epidemik tarzda genellikle gebeliğin son trimesterinde görülen abort olgularından sorumlu başlıca etkendir. Bu araştırmada, yeni dizayn edilen bir primer çifti kullanılarak, ülkemizde 2011 yılındaki doğum sezonundaki abort salgınından izole edilen Türkiye (TR) EHV-1 saha suşlarının glikoprotein B (gB) gen bölgesinin kısmi karakterizasyonu araştırılmıştır. Moleküler analiz sonuçları bu araştırmada izole edilen suşların, önceki yıllarda ülkemizde izole edilen EHV-1 suşlarına genetik olarak benzer; EHV-1 Avrupa suşlarıyla ise genetik yakınlığa sahip olduğunu göstermiştir. Ayrıca, bu araştırmada izole edilen suşlar ile GenBank veri tabanında kayıtlı diğer EHV-1 suşlarının aminoasit sekansları arasında farklılıklar tespit edilmiştir. Bu moleküler araştırma, ülkemizde EHV-1 enfeksiyonunun izlenmesi ve gelecekteki salgınlardan izole edilebilecek yeni suşların gB gen bölgesindeki değişikliklerin patogenezi ve hastalığın gelişimi üzerine etkilerinin belirlenmesine ışık tutacaktır.