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Öğe Comparative Efficacy of Three Anesthetic Agents in Himri Barbel, Carasobarbus luteus (Heckel, 1843) under Controlled Conditions(Medwell Online, 2011) Gokcek, Kaya; Ogretmen, FatihIn this study, the efficacy of three anaesthetic agents (Clove oil, 2-phenoxyethanol and benzocaine) was compared in captive-bred Himri barbel, Carasobarbus luteus (Heckel, 1843). The lowest effective concentrations based on the efficacy criteria of complete anesthesia induction within 180 sec and recovery within 300 sec were determined to be 75 mg L-1 (induction 143 +/- 15 sec and recovery time 149 +/- 35 sec) for clove oil, 500 mu L L-1 (induction 145 +/- 27 sec and recovery time 57 +/- 16 sec) for 2-phenooxyethanol and 50 mg L-1 (induction 152 +/- 49 sec and recovery time 88 +/- 24 sec) for benzocaine. The onset of individual phases of anesthesia and recovery times depended significantly on the concentration of the anaesthetic used (p<0.05). An inverse exponential relationship was observed between concentrations of anaesthetic and induction time whereas exponential relationships were observed between concentrations and recovery times for all anaesthetic agents evaluated,Öğe Comparing the Effectiveness of Ovopel and Carp Pituitary Extract (CPE) on Artificial Spawning of Scaly Carp (Cyprinus carpio)(Medwell Online, 2010) Kayim, Murathan; Bozkurt, Yusuf; Ogretmen, FatihThe effect of ovulation stimulators (Ovopel and carp pituitary extract) on spawning of scaly carp females were investigated. Following application of Ovopel, eggs were obtained from higher percentage of females than CPE treatment (92.5 and 75.0%, respectively. In the case of Ovopel stimulator, female weights were significantly correlated with living embryo (r = 0.576, p<0.01) and hatching rates (r = 0.684, p<0.01). Also interaction between egg weights and fertilization rates (r = 0.603, p<0.01) and between hatching and living embryo rates (r = 0.679, p<0.01) were determined significant. On the other hand, egg weights were negatively correlated (r = -0.368, p<0.01) with living embryo rates. For the CPE stimulator, egg weights were significantly correlated with fertilization (r = 0.662, p<0.01), living embryo (r = 0.390, p<0.05) and hatching rates (r = 0.443, p<0.01). Also hatching were significatly correlated with fertilization (r = 0.665, p<0.01) and living embryo rates (r = 0.903, p<0.01). In addition, fertilization and living embryo rates significantly correlated (r = 0.615, p<0.01) with each other.Öğe Effect of Glycerol on Fertility of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm(Israeli Journal Of Aquaculture-Bamidgeh, 2011) Bozkurt, Yusuf; Yavas, Ilker; Ogretmen, Fatih; Sivasligil, Bugra; Karaca, FikretThis research investigated the effect of adding glycerol to different ionic extenders on motility and fertilizing ability of frozen-thawed sperm of grass carp (Ctenopharyngodon idella) under hatchery conditions. Semen was collected by abdominal stripping from adult males and diluted to a ratio of 1:3 in Kurokura I or Kurokura II extender containing 10%, 15%, or 20% glycerol. Diluted semen was packaged in 0.5-ml straws and left to equilibrate for 30 min at 4 degrees C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into liquid nitrogen (-196 degrees C). Straws were thawed in a water bath at 30 degrees C for 10 s to determine the post-thaw motility and movement duration. The spermatozoa: egg ratio in fertilization experiments was 1 x 10(5):1. The highest mean fertilization rate (95.63 +/- 0.52; p>0.05) was obtained with semen frozen in Kurokura I extender containing 10% glycerol. Results indicate that grass carp sperm can be successfully cryopreserved with ionic extenders containing glycerol at 10-20%.Öğe Effects of Seminal Plasma Composition on Sperm Motility in Mirror Carp (Cyprinus carpio)(Israeli Journal Of Aquaculture-Bamidgeh, 2009) Bozkurt, Yusuf; Ogretmen, Fatih; Secer, Faik Sertel; Ercin, UgurThis research provides data concerning the biochemical (ionic and organic) composition of sperm of the mirror carp (Cyprinus carpio) and its relationship to spermatozoa motility. Seminal plasma contained 67.12 +/- 1.06 mM/l Na+, 105.1 +/- 2.24 mM/l K+, 7.85 +/- 0.67 mg/dl Ca(2+), 2.61 +/- 0.11 mEq/l Mg(2+), 0.14 +/- 0.002 g/dl total protein, 10.3 +/- 1.01 mg/dl triglyceride, 6.83 +/- 0.72 mg/dl cholesterol, and 54.72 +/- 3.49 mg/dl urea. A positive relationship (p<0.05) was determined between Na(+) and motility (r = 0.522). On the other hand, Ca(2+), K(+), and Mg(2+) ions negatively correlated (p>0.05) with motility (r = -0.565, r = -0.160, and r = -0.184, respectively). Spermatozoa motility correlated negatively (p>0.05) with protein (r = -0.233), triglyceride (r = -0.348), and urea (r = -0.331) but positively with cholesterol (r = 0.012). This information will help to develop cryopreservation procedures, to meet species-specific extender requirements, and to optimize artificial fertilization procedures in mirror carp.Öğe Efficacy of Clove Oil, 2-Phenoxyethanol and Benzocaine on European Catfish, Silurus glanis Linnaeus 1758(Central Fisheries Research Inst, 2017) Gokcek, Kaya; Ogretmen, Fatih; Kanyilmaz, MahirThe efficacy of clove oil, 2-phenoxyethanol and benzocaine was tested on captive-bred European catfish (wels), Silurus glanis L., 1758 in this study. According to effectiveness criteria, the most optimal implementation results were obtained from 100 mu l L-1 (induction 69.00 +/- 6.48 s and recovery 253.86 +/- 14.78 s) clove oil, 1000 mu l L-1 (induction 88.71 +/- 8.50 s and recovery 201.43 +/- 12.19 s) PE, and 100 mu l L-1 (induction 103.00 +/- 6.90 s and recovery 215.71 +/- 19.99 s) benzocaine. The concentration of anesthetics agent used directly affected the onset of each physiological respond (p<0.05). The relationship between doses and induction times was inverse exponential, in spite of that an exponential relationship was confirmed between doses and recovery times for all anesthetics. In conclusion, all these three anesthetics can be effectively used for wels.Öğe Relationship Between Seminal Plasma Composition and Spermatological Parameters in Scaly Carp (Cyprinus carpio)(Medwell Online, 2009) Bozkurt, Yusuf; Ogretmen, Fatih; Secer, Faik Sertel; Ercin, UgurThe present study was conducted to evaluate seminal plasma composition, spermatological parameters and their relationships in scaly carp (Cyprinus carpio). Seminal plasma contained 71.18 +/- 3.40 mML(-1) (Na(+)), 87.48 +/- 4.42 mML(-1) (K(+)), 10.64 +/- 1.50 mg dL(-1) (Ca(2+)), 2.57 +/- 0.19 mEq L(-1) (Mg(2+)), 0.12 +/- 0.04 g dL(-1) total protein, 1.43 +/- 0.02 mg dL(-1) glucose, 7.82 +/- 2.60 mg dL(-1) triglyceride, 6.27 +/- 2.32 mg dL(-1) cholesterol, 24.45 +/- 7.98 mg dL(-1) urea. The following spermatological parameters were found: sperm volume 13.17 +/- 5.39 mL, sperm motility 63.18 +/- 7.16%, movement duration 56.81 +/- 20.32 s, sperm concentration 29.637 +/- 1.07 x 10(9) mL(-1), total sperm concentration 419.200 +/- 29.62 x 10(9) and pH 7.59 +/- 0.74. The K(+) and Ca(2+) ions correlated positively with motility (r = 0.215, p>0.05 and r = 0.048, p>0.05), respectively. On the other hand, Na(+) and Mg(2+) ions correlated negatively with motility (r = -0.067, p>0.05, r = -0.407, p>0.05), respectively. Significant correlations were observed between K(+) and Na(+) (r = -0.695, p<0.05), protein and triglyceride (r = 0.786, p<0.01), protein and cholesterol (r = 0.936, p<0.01), triglyceride and cholesterol (r = 0.802, p<0.01). These parameters can be used to prepare species-specific extenders for short-term storage and cryopreservation of scaly carp sperm.Öğe Short-Term Preservation of Grass Carp (Ctenopharyngodon idella) Eggs: Effect of Extenders and Storage Duration(Israeli Journal Of Aquaculture-Bamidgeh, 2010) Bozkurt, Yusuf; Ogretmen, Fatih; Secer, Faik SertelThe short-term effects of extenders and storage duration on fertilization, eyeing, and hatching rates in short-term stored grass carp eggs was evaluated. Eggs were kept in one of two artificial extenders (Ringer and 300 mM glucose solutions) or in ovarian fluid in 10-cm petri dishes. About 4 ml of artificial extender or coeolomic fluid was added to each petri dish and the dishes were transferred to a dark humid refrigerator for storage at 4 C for 120, 240, 360, or 480 min. Eggs were fertilized by adding 200 mu l milt to each petri dish. The fertilization rate was determined at the gastrula stage. The eyeing rate was defined as the number of eyed eggs divided by the initial number of eggs used for fertilization. The highest fertilization rate (93%) was attained in coelomic fluid stored for 120 min. The highest eyeing rates were in eggs kept in ovarian fluid for 120 or 240 min. The glucose extender was best for longer storage (360 and 480 min). The highest hatching rate was obtained with ovarian fluid at 240 min but the glucose extender was best for storage at 120, 360, and 480 min. Results indicate that glucose solution is the best extender for short-term (2-4 h) storage of grass carp eggs.