Yazar "Oguzoglu, Tuba Cigdem" seçeneğine göre listele

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    The detection of feline coronaviruses in blood samples from cats by mRNA RT-PCR
    (Sage Publications Ltd, 2007) Can-Sahna, Kezban; Ataseven, Veysel Soydal; Pinar, Dilek; Oguzoglu, Tuba Cigdem
    In this study, 26 blood samples were collected from 25 healthy cats and one cat with clinical signs suggestive of feline infectious peritonitis (FIP), namely, fever, weight loss, enlarged abdomen, and ascites. Blood samples were tested for feline coronavirus (FCoV) messenger RNA (mRNA) by an reverse transcriptase-polymerase chain reaction (RT-PCR) assay which has previously been described to have a high specificity in the diagnosis of clinical FlP [Simons AF, Vennema H, Rofina JE, Pol JM, Horzinek MC, Rottier PJM,Egberink HF (2005) A mRNA PCR for the diagnosis of feline infectious peritonitis. Journal of Virological Methods 124, 111-116]. Overall we found 14 (54%) of the cats were positive for FCoV including the cat with clinical disease, but the high rate of positivity among healthy cats suggested a poor specificity for the clinical (diagnosis of FlP among these cats. it was observed that the positivity rate was highest in cats aged between 6 months-1year old. Our findings suggest that FCoVs may be present in the blood samples from healthy cats as well as cats with clinical FIR (c) 2007 ESFM and AAFP. Published by Elsevier Ltd. All rights reserved.
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    Endless variety for bovine virus diarrhea viruses: new members of a novel subgroup into Pestivirus A from Turkey
    (Springer, 2019) Oguzoglu, Tuba Cigdem; Koc, Bahattin Taylan; Coskun, Nuvit; Dogan, Firat; Duran-Yelken, Selda
    As ubiquitous pathogens, bovine virus diarrhea viruses (BVDVs) in cattle have been reported several times in Turkey. Over time, the frequency and importance of this infection has increased for the livestock industries. A total of 1291 animals were sampled from a dairy herd in Turkey suspected of BVDV clinical signs, for instance, reproductive failures (abortion, congenital malformations in calves, repeat breeding, etc.) and interdigital phlegmon in adult animals. Reverse transcription polymerase chain reactions (RT-PCRs) were made by using targeted 5' untranslated region (UTR), E2, and NS2-3 pestiviral gene region primers for antigen ELISA-positive samples (n = 20). The obtained amplicons were sequenced. Sequence results showed the presence of a new subgroup in Pe.stivirus A species. This paper describes the nucleotide sequences of a new BVDV 1 (BVDV 1v) subgroup member.
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    First genetic characterization of equine adenovirus type 1 (EAdV-1) in Turkey
    (Elsevier Sci Ltd, 2012) Ataseven, Veysel Soydal; Oguzoglu, Tuba Cigdem; Basaran-Karapinar, Zeynep; Bilge-Dagalp, Seval
    Equine adenovirus type 1 (EAdV-1) is a cause of repiratory tract infection in equids. In present study for the first time in Turkey, the prevalence of EAdV-1 in nasal swab samples obtained from horses showing respiratory symptoms was investigated by polymerase chain reaction (PCR), and molecular characterization of the hexon gene detected in the Turkish (TR) strain was performed. Overall, the prevalence of EAdV-1 was found low (1.4%) as indicated by a positive PCR reaction from the nasal swab extracts tested. Phylogenetic analysis based on the partial sequences of the hexon gene of a TR-EAdV-1 strain with those of previously isolated AdVs from different mammals and an EAdV-1 M1 strain showed that the EAdV-1 strains were placed into a unique cluster. Although the TR-EAdV-1 strain was closely related to CAV-1, CAV-2 and bat adenovirus reference strains, larger-scale studies are necessary to better understand the molecular epidemiology and population structure of EAdV-1 in Turkey. (C) 2011 Elsevier Ltd. All rights reserved.
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    First molecular characterization of feline immunodeficiency virus in Turkey
    (Springer Wien, 2010) Oguzoglu, Tuba Cigdem; Timurkan, Mehmet Ozkan; Muz, Dilek; Kudu, Aysegul; Numanbayraktaroglu, Basak; Sadak, Seda; Burgu, Ibrahim
    In this study, strains of feline immunodeficiency virus (Fly), designated TR-D, TR-Mo and TR-Mi, isolated from three cats in Turkey, were characterized. PCR products (859 bp) from the envelope (env) gene region were amplified and sequenced, and possible geographical differences in the env gene region of Turkish FIV strains are discussed. Phylogenetic analysis of two strains showed that FIV subtype B was present in Turkey. Phylogenetic analysis showed that one new Turkish FIV strain occupies a separate branch from known clusters (subtypes A to E) from the USA, Canada, Europe and Japan.
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    First molecular characterization of visna/maedi viruses from naturally infected sheep in Turkey
    (Springer Wien, 2013) Muz, Dilek; Oguzoglu, Tuba Cigdem; Rosati, Sergio; Reina, Ramses; Bertolotti, Luigi; Burgu, Ibrahim
    Recent worldwide serological and genetic studies of small ruminant lentiviruses (SRLV) have led to the description of new genotypes and the development of new diagnostic tests. This study investigated the detection and molecular characterization of visna/maedi virus (VMV) infection in serum and blood samples from pure and mixed sheep breeds acquired from different regions in Turkey using ELISA and PCR techniques. The prevalence of VMV was 67.8 % by ELISA and/or LTR-PCR with both assays showing a medium level of agreement (kappa: 0.26; +/- A 0.038 CI). Positivity of VMV in sheep increased according to the age of the animal, although PCR positivity was higher than ELISA in young individuals. Phylogenetic analysis of 33 LTR sequences identified two distinct clades that were closely related to American and Greek LTR sequences. Phylogenetic analysis of 10 partial gag gene sequences identified A2, A3, A5, A9, A11 subtypes of genotype A SRLVs. In vitro culture of all isolates in fetal sheep lung cells (FSLC) showed a slow/low phenotype causing less or no lytic infection compared with infection with the WLC-1 American strain characterized by a rapid/highly lytic phenotype. Phylogenetic analysis revealed that Turkish VMV sequences preceded the establishment of American or Greek strains that were associated with the migration of sheep from the Middle East to Western Europe several centuries ago. This is the first study that describes Turkish VMV sequences with the molecular characterization of LTR and gag genes, and it strongly suggests that SRLV-genotype A originated in Turkey.
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    Molecular characterization of Bovine virus diarrhea viruses species 2 (BVDV-2) from cattle in Turkey
    (Springer, 2010) Oguzoglu, Tuba Cigdem; Muz, Dilek; Yilmaz, Volkan; Alkan, Feray; Akca, Yilmaz; Burgu, Ibrahim
    Five BVDV species 2 (BVDV-2) isolates were detected from cattle in Turkey. Phylogenetic analysis was performed based on the 5' untranslated regions (UTRs) and E2 coding gene regions, respectively. The isolates were closely related to BVDV-2a strains from North America and Canada used as references. This is the first report of the detection of BVDV-2 in naturally infected Turkish cattle. It is important to consider BVDV-2 for planning future BVDV control and vaccination programs in Turkey.
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    Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey
    (Ankara Univ Press, 2016) Ataseven, Veysel Soydal; Oguzoglu, Tuba Cigdem; Dincer, Ender; Bilge Dagalp, Seval
    Equid herpesvirus 1 (EHV-1) is a major agent of large-scale outbreaks of abortion, and these abortions have been described as sporadic or epidemic cases in mares, generally during the last trimester of pregnancy. In this study, the partial characterization based on the glycoprotein B (gB) gene of Turkish (TR) EHV-1 field strains isolated from an abortion outbreak during the 2011 foaling season in Turkey was investigated using a novel designed primer set for EHV-1. The molecular analysis of TR EHV-1 strains showed that genetically identical TR EHV-1 strains were still circulating in Turkey during different years and, these strains were closely related to the European EHV-1 strains. Furthermore, EHV-1 isolated in the present study and to EHV-1 strains published previously in the GenBank database were shown some differences for the aa sequences. This molecular report would be valuable for monitoring of EHV-1 infection in Turkey and determining the gB gene sequence of newly identified EHV-1 field strains from future outbreaks on the pathogenesis and severity of disease.