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    The frequency of shiga-like toxin (stx1 and stx2) and EHEC-hlyA in food by multiplex PCR
    (Univ Press, 2017) Onlen, Cansu; Duran, Nizami; Bayraktar, Suphi; Ay, Emrah; Ozer, Burcin
    Aim: The aim of the present study was to determine the frequency of shiga-like toxin (stx1 and stx2) and drug resistance profiles food-borne Escherichia coli O157: H7 in Hatay province, Turkey. Methods: The presence of the virulence genes (stx1, stx2, hlyA) in a total of 150 E. coli isolates were studied with multiplex PCR. Results: A total of 327 salad samples were analyzed. E. coli O157: H7 was detected in 150 (45.8 %) out of 327 analyzed samples. Of these 150 isolates, the presence of hly-A gene was detected in 32 (21.3%) E. coli isolates. A total of five (15.6%) isolates in this 32 hlyA positive isolates had stx2 gene, two (6.3%) of them had stx1 gene and one (3.1%) of the isolates was found to be positive for both stx1 and stx2 genes. It was found that all E. coli O157: H7 isolates were resistant to erythromycin. While the highest rate of antibiotic resistance was observed for ampicillin (68.8%), no antibiotic resistance against cefuroxime, ciprofloxacin and cephaperasone was identified. Conclusions: The results obtained in our province showed that E. coli strains isolated from salad samples were found to have some important virulence genes such as stx1, stx2, and hlyA. The stx2 frequency was found to be higher than stx1 frequency. Also, it was observed that there was not any significant correlation between drug resistance profiles and presence of toxin genes in E. coli O157: H7 strains. As a result, increasing frequency of STEC O157 serotype among foodborne pathogens is a growing public health problem.
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    Identification of Medically Important Candida Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of the rDNA ITS1 and ITS2 Regions
    (Elsevier, 2017) Bayraktar, Suphi; Duran, Nizami; Duran, Gulay Gulbol; Eryilmaz, Naciye; Aslan, Hayat; Onlen, Cansu; Ozer, Burcin
    Aim: We aimed to identify the distribution of species in candidal strains isolated from clinical samples and restriction fragment length polymorphism (RFLP) method based on Msp I and Bln I restrictive enzyme cuts of polymerase chain reaction (PCR) products after the amplification of ITS1 and ITS2 regions of rDNA genotypically. Materials and Methods: One hundred and fifty candidal strains isolated from various clinical samples were studies/ included. Phenotypic species assessment was performed using automated VITEK-2 system and kit used with the biochemical tests. Common genomic region amplification peculiar to candidal strains was carried out using ITS1 and ITS2 primer pairs. After the amplification, PCR products were cut with Msp I and Bln I restriction enzymes for species identification. Results: The majority of Candida isolates were isolated from urine (78.6%) while other isolates were composed of strains isolated from swab, wound, blood and other samples by 11.3%, 3.3%, 2% and 4.7%, respectively. The result of RFLP analysis carried out with Msp I and Bln I restriction enzymes showed that candidal strains were Candida albicans by 45.3%, Candida glabrata by 19.3%, Candida tropicalis by 14.6%, Candida parapsilosis by 5.3%, Candida krusei by 5.3%, Candida lusitaniae by 0.6% and other candidal strains by 9.3%. Conclusion: When the ability to identify Candida to species level of phenotypic and PCR-RFLP methods was assessed, a great difference was found between these two methods. It may be argued that Msp I and Bln I restriction enzyme fragments can be used in the identification of medically important Candida species. Further studies are needed to develop this kind of restriction profile to be used in the identification of candidal strains.
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    The relationship between acute coronary artery diseases with c-reactive protein +1059 G/C and angiotensin-converting enzyme I/D gene polymorphisms
    (E-Century Publishing Corporation, 2016) Duran, Gulay Gulbol; Fansa, Iyad; Duran, Nizami; Jenedi, Kemal; Onlen, Cansu; Miraloglu, Meral; Yigin, Akin
    Objective: The purpose of this study was to evaluate the presence of an association between the CRP +1059 G/C and ACE I/D gene polymorphisms and patients who were diagnosed to have acute coronary syndrome and underwent coronary angiography. Methods: A total of 126 patients (mean age: 60.0±12.9) and 144 healthy individuals (mean age: 52.1±13.0) were included to this study. The presence of CRP +1059 G/C and ACE I/D gene polymorphisms were analyzed using the RFLP method. Results: When the patient and control groups were evaluated in terms of ACE I/D gene polymorphism, no statistically significant difference was found in the frequency of ACE DD and ACE ID between the two groups (P>0.05), while the percentage of ACE II genotype was statistically significantly higher in the patient group compared with the control group (P<0.032). For the distribution of CRP G/C genotype; CRP GG, CRP GC and CRP CC genotype frequencies were similar in the patient and control groups (P>0.05). When the presence of the ACE I/D genotype and CRP G/C genotype was compared in patients with vessel disease (one vessel, two vessels and three vessels) among the patients with coronary artery diseases with the control group, statistically significant differences were found between the two groups (P<0.05). In addition, the frequency of the ACE I/D genotype in hypertensive patients with coronary artery disease was statistically significantly higher (P<0.033). Also, the frequency of the CRP +1059 G/C genotype was found to be statistically significantly higher in the patient group (P<0.026). Conclusion: This study demonstrated that CRP +1059 G/C and ACE I/D gene polymorphisms may be a genetic marker associated with coronary artery disease in patients diagnosed with ACS. © 2016, E-Century Publishing Corporation. All rights reserved.
  • [ N/A ]
    Öğe
    The relationship between acute coronary artery diseases with c-reactive protein+1059 G/C and angiotensin-converting enzyme I/D gene polymorphisms
    (E-Century Publishing Corp, 2016) Duran, Gulay Gulbol; Fansa, Iyad; Duran, Nizami; Jenedi, Kemal; Onlen, Cansu; Miraloglu, Meral; Yigin, Akin
    Objective: The purpose of this study was to evaluate the presence of an association between the CRP + 1059 G/C and ACE I/D gene polymorphisms and patients who were diagnosed to have acute coronary syndrome and underwent coronary angiography. Methods: A total of 126 patients (mean age: 60.0 +/- 12.9) and 144 healthy individuals (mean age: 52.1 +/- 13.0) were included to this study. The presence of CRP + 1059 G/C and ACE I/D gene polymorphisms were analyzed using the RFLP method. Results: When the patient and control groups were evaluated in terms of ACE I/D gene polymorphism, no statistically significant difference was found in the frequency of ACE DD and ACE ID between the two groups (P>0.05), while the percentage of ACE II genotype was statistically significantly higher in the patient group compared with the control group (P<0.032). For the distribution of CRP G/C genotype; CRP GG, CRP GC and CRP CC genotype frequencies were similar in the patient and control groups (P>0.05). When the presence of the ACE I/D genotype and CRP G/C genotype was compared in patients with vessel disease (one vessel, two vessels and three vessels) among the patients with coronary artery diseases with the control group, statistically significant differences were found between the two groups (P<0.05). In addition, the frequency of the ACE I/D genotype in hypertensive patients with coronary artery disease was statistically significantly higher (P<0.033). Also, the frequency of the CRP + 1059 G/C genotype was found to be statistically significantly higher in the patient group (P<0.026). Conclusion: This study demonstrated that CRP + 1059 G/C and ACE I/D gene polymorphisms may be a genetic marker associated with coronary artery disease in patients diagnosed with ACS.