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Öğe Antibacterial activity of calcium hydroxide combined with triple antibiotic paste against enterococcus faecalis; An in vitro study(2013) Kusgoz, Adem; Ozcan, Erhan; Arslan, Ipek; Inci, MelekObjectives: The aim of the present in vitro study was to investigate the antibacterial efficacy of calcium hydroxide mixed with triple antibiotic paste at various rates. Materials and Methods: Triple antibiotic paste (TAP; metronidizol, minocycline, ciprofloxacin) and calcium hydroxide powder was mixed in different proportions and tested for antibacterial activity against E. faecalis (ATCC 29212)using the agar diffusion method. The samples were divided into 10 different groups;Group 1: 100% Ca(OH)2, Group 2: 99,5% Ca(OH)2 + 0.5% TAP, Group 3: 99% Ca(OH)2 + 1%TAP, Group 4: 97,5% Ca(OH)2 + 2,5% TAP, Group 5: 95% Ca(OH)2 + 5%TAP, Group 6: 90% Ca(OH)2 + 10%TAP, Group 7: 75% Ca(OH)2 + 25% TAP, Group 8: 50% Ca(OH)2 + 50% TAP, Group 9: 25% Ca(OH)2 + 75% TAP, Group 10: %100 TAP. Standard holes in the cultivated agar plates were filled with one of the samples and the zones of microbial inhibition were measured after incubation period (48 hours). The experiment was performed 8 times with each medicament. Results: Group 1 had no effect against E.faecalis. Group 10 demonstrated high antibacterial activity than any other groups in this study. The antibacterial effects of the groups could be ranked from strongest to weakest as follows: Group 10, 9, 8, 7, 6, 5, 4, 3, 2, 1. Conclusions: Adding the triple antibiotic paste increased he antibacterial efficacy of calcium hydroxide. In addition, the antibacterial effect of calcium hydroxide also increased with higher proportion of TAP.Öğe Antifungal activity of several root canal sealers against Candida albicans(Taylor & Francis Ltd, 2013) Ozcan, Erhan; Yula, Erkan; Arslanoglu, Zeki; Inci, MelekObjective. The purpose of this in vitro study was to evaluate the antifungal activity of several root canal sealers (iRoot SP, MTA Fillapex and GuttaFlow) against Candida albicans and compare them to that of AH Plus Jet. Materials and methods. A10 mu L fungi in suspension was allowed to directly contact the sealers, which were freshly mixed or allowed to set for 1 or 7 days. Fresh media was then added and survival of fungi was determined by using 10-fold serial dilution and inoculated onto agar plates. After incubation for 48 h, colony-forming units (CFU) were calculated and their log(10) values converted. The data were analyzed using ANOVA and Tukey tests (alpha = 0.05). Results. Freshly mixed AH Plus Jet totally inhibited the growth of fungi and showed the highest antifungal activity. GuttaFlow did not show any significant antifungal activity at all times. Freshly mixed iRoot SP and MTA Fillapex were found to be antifungal. Statistical differences were found between freshly mixed and set samples (p < 0.05) in favor of the former, except GuttaFlow. No statistically significant differences were found among the tested sealers at the 1 and 7-day samples (p > 0.05). Conclusions. Fresh AH Plus Jet had very potent antifungal activity. All sealers, except GuttaFlow, exhibited antifungal activity when freshly mixed.Öğe Antimicrobial effects of several calcium silicate-based root-end filling materials(Japanese Soc Dental Materials Devices, 2014) Damlar, Ibrahim; Ozcan, Erhan; Yula, Erkan; Yalcin, Muhammet; Celik, SalihThe purpose of this study was to evaluate, in vitro, the antimicrobial effect of iRoot BP, iRoot BP Plus, and mineral trioxide aggregate (MTA) against Entercoccus faecalis and Candida albicans by using direct contact test. The materials were tested immediately after application to the microtiter wells and after setting for 1-day and for 7-days. Ten microliters of microbial suspension was added to each well for direct contact with each material for 1 h at 37 degrees C and 100% humidity. Then fresh media was added and, survival of bacteria and fungi was determined by using 10-fold serial dilution and inoculated onto agar plates. In fresh and 1-day samples all of tested materials showed statistically significant antimicrobial effects compared to control groups (p<0.05). In 7-day samples, there were no significantly differences compared to control groups. MTA, iRoot BP and iRoot BP Plus had similar antimicrobial efficacy against E. faecalis and C. albicans.Öğe The effect of luting cement thicknesses on the push-out bond strength of the fiber posts(Taylor & Francis Ltd, 2013) Ozcan, Erhan; Cetin, Ali Riza; Tuncdemir, Ali Riza; Ulker, MustafaObjective. The aim of this study was to evaluate the effect of luting cement thicknesses on the push-out bond strength of the fiber posts. Materials and methods. Sixty human maxillary canine teeth were endodontically treated. Post spaces were prepared and root specimens randomly divided into three groups: Group 1: etch-and-rinse (Variolink II/Exite DSC), Group 2: self-etch (Panavia F2.0/ED primer) and Group 3: self-adhesive (Clearfil SA Cement). Then each group was divided into four sub-groups according to the cement thickness, as follows: Sub-group 1: 0.35 mm, Sub-group 2: 0.25 mm, Sub-group 3: 0.15 mm, and Sub-group 4: 0.05 mm. Three slices of 1 mm thickness were obtained from each root specimen (n = 15). Push-out tests were performed and data was analyzed with two-way ANOVA and Tukey tests. Results. The etch-and-rinse system (Variolink II) had a significantly higher bond strength compared with the other systems (p < 0.05). The thickest cement group (0.35 mm) had a significantly lower bond strength compared with the 0.15 and 0.05 mm groups in the etch-and-rinse and self-adhesive groups (p < 0.05). In the self-etch group, the cement thicknesses did not significantly affect the bond strength (p > 0.05). Conclusions. The etch-and-rinse system offered better bonding to root dentine than the self-adhesive and self-etch systems. The increases in cement thickness significantly reduced the bond strength of fiber posts to root dentine for both the etch-and-rinse and self-adhesive systems.Öğe The effect of post surface treatments on the bond strength of fiber posts to root surfaces(Springer London Ltd, 2013) Tuncdemir, Ali Riza; Yildirim, Cihan; Guller, Fatma; Ozcan, Erhan; Usumez, AslihanThe aim of the present study was to evaluate the influence of post surface treatment methods on the push-out bond strength of adhesively luted quartz fiber posts. Thirty freshly extracted and endodontically treated human incisor teeth were prepared for quartz fiber posts. The posts were submitted to three different surface treatments (n = 10), including no treatment, 50-A mu m aluminum-oxide (Al2O3) airborne-particle abrasion and Er:YAG laser (10 Hz, 150 mJ) irradiation. The posts were luted with resin cement. Each root was sectioned perpendicularly to its long axis to create specimens of 1-mm thickness. After the specimens were stored in distilled water at 37A degrees C for 24 h, their push-out bond strength was tested using a universal testing machine at a crosshead speed of 1 mm/min. The data were analyzed by two-way ANOVA (alpha = .05). The two-way ANOVA indicated that push-out test values did not vary significantly according to surface treatments applied (control, airborne particle abrasion, Er:YAG laser irradiation) (p > 0.05), however, values varied according to the root segments (cervical, middle, and apical) (p < 0.01). The push-out bond strength values of the coronal root sections were the highest (p < 0.05) and there were no significant differences between the middle and apical root sections in push-out bond strength of fiber posts (p > 0.05). Air-borne particle abrasion or Er:YAG laser irradiation applied on the quartz fiber posts did not affect the push-out bond strengths relative to the root surfaces. The highest bond strength was observed in the cervical third of the roots in all groups.Öğe Effects of different intracoronal bleaching methods on shear bond strengths of orthodontic brackets(E H Angle Education Research Foundation, Inc, 2012) Gungor, Ahmet Yalcin; Ozcan, Erhan; Alkis, Huseyin; Turkkahraman, HakanObjective: To evaluate the effects of different intracoronal bleaching methods on the shear bond strengths (SBS) of orthodontic brackets. Materials and Methods: Sixty freshly extracted mandibular incisors were randomly divided into four groups (n = 15 per group). After filling the root canals, root fillings were removed 2 mm apical to the cementoenamel junction, and a 2-mm-thick layer of zinc-phosphate cement base was applied. Group 1 served as the control. Intracoronal bleaching was performed with hydrogen peroxide (Opalacence Endo, Ultradent products Inc, South Jordan, Utah) in group 2, sodium perborate (Sultan Healthcare, Englewood, NJ) in group 3, and 37% carbamide peroxide (Whiteness Super Endo, Dentscare, Itda, Joinville, Brazil) in group 4. Orthodontic brackets were bonded with a light cure composite resin and cured with an LED light. After bonding, the SBS of the brackets was tested with a Universal testing machine. Results: Analysis of variance indicated a significant difference between groups (P < .001). The highest values for SBS were measured in group 1 (10.15 +/- 1.15 MPa). The SBS was significantly lower in groups 2,3, and 4 than in group 1 (P < .001). The lowest values for SBS were measured in group 3 (6.17 +/- 0.85 MPa). SBS was significantly higher in group 4 than in group 3 (P < .05). Conclusions: Intracoronal bleaching significantly affected the SBS of orthodontic brackets on human enamel. Bleaching with sodium perborate affects SBS more adversely than does bleaching with other agents. (Angle Orthod. 2012;82:942-946.)Öğe Influence of eugenol on the push-out bond strengths of fiber posts cemented with different types of resin luting agents(Springer, 2013) Ozcan, Erhan; Cetin, Ali Riza; Capar, Ismail Davut; Tuncdemir, Ali Riza; Aydinbelge, Hale AriThis study evaluated the influence of eugenol on the push-out bond strengths of fiber posts cemented with different types of resin luting agents. Seventy-two extracted maxillary single-rooted canine teeth were randomly divided into two groups of 36 teeth. Group 1, the control group, was filled with gutta-percha only (i.e., did not receive eugenol), whereas group 2 was filled with a eugenol-containing sealer. All root canals were filled and each group was divided into three subgroups. The posts in each subgroup were cemented with the following materials: subgroup 1 with a 2-step self-etching adhesive system (Clearfil Liner Bond 2V + Panavia F); subgroup 2 with a 1-step self-etching adhesive (Panavia F); and subgroup 3 with a self-adhesive (Clearfil SA Cement). Dislodgement resistance was measured using a universal testing machine. All data were subjected to ANOVA using a factorial design and Tukey test (alpha = 0.05). The use of the eugenol-containing sealer significantly reduced the push-out bond strength of the fiber post (P < 0.05). The push-out bond strength of Panavia F was significantly higher than those of the other groups filled with the eugenol-containing sealer (P < 0.05). The Panavia F group was less susceptible to the inhibiting effect of eugenol than were the other evaluated groups when the fiber post was cemented in the canals filled with the eugenol-containing sealer.
