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Öğe CHANGES IN CHEMICAL COMPOSITION, ANTIOXIDANT ACTIVITIES AND TOTAL PHENOLIC CONTENT OF ARBUTUS ANDRACHNE FRUIT AT DIFFERENT MATURATION STAGES(Chiriotti Editori, 2009) Ozgen, M.; Torun, A. A.; Ercisli, S.; Serce, S.Several characteristics of Arbutus andrachne L. fruit harvested at four maturation stages (green, green-orange, orange-red and red) have been examined. Soluble solids, pH, acidity, organic acids, specific sugars, and several nutrient element concentrations (N, P, K, Ca, Mg, Fe, Zn, Mn, Cu) have been determined. The total phenolic content along with antioxidant activity, determined by FRAP and TEAC methods, were also measured. There were significant differences among most of the traits tested. The average soluble solids increased from 16.0 to 20.6% going from the green to the red stages, while the acidity decreased from 0.54 to 0.45%. All of the element concentrations were statistically different among the various stages, with N and Ca being the most variable. The total acidity was found to be the highest in the orange-red and red stages (0.52 and 0.50 g/100 g, respectively). The main organic acid was malic acid (0.32 g/100 g) followed by ascorbic and citric acids (0.09 and 0.03 g/100 g) respectively. The red (17.84 g/100 g) and orange-red (16.25 g/100 g) stages had the highest total sugar values. Fructose was the main sugar in the fruit with an overall average of 8.91 g/100 g. The total phenolic content was highest at the red stage (3,904 mg gallic acid (GA)/kg fresh weight (fw)), followed by the green stage (3,589 mg GA/kg fw). The antioxidant activities were highest at the red stage as determined by both the TEAC and FRAP methods (26.5 and 21.8 mu mol TE/g fw).Öğe Relationships among Crataegus accessions sampled from Hatay, Turkey, as assessed by fruit characteristics and RAPD(Springer, 2011) Serce, S.; Simsek, O.; Toplu, C.; Kamiloglu, O.; Caliskan, O.; Gunduz, K.; Ozgen, M.The genus Crataegus known as hawthorns, is the largest genus among the Maloideae, which comprises 265 species. Turkey is one of the genetic centers of Crataegus and there are more than 20 species found in Turkey. The fruits of Crataegus are used as food and have high flavonoid, vitamin C, glycoside, anthocyanidin, saponin, tannin, and antioxidant levels. In this study, we attempted to characterize 15 Crataegus accessions sampled from Hatay, located in Eastern Mediterranean region of Turkey. The accessions belonged to several species; C. aronia (L.) DC. var. aronia, C. aronia var. dentata Browicz, C. aronia var. minuta Browicz, C. monogyna Jacq. subsp. azarella (Griseb.) Franco, and C. orientalis Pall. ex M. Bieb. var. orientalis. Fruit characteristics of the accessions exhibited considerable variation. The multivariate, principle component and cluster analyses indicated that the accessions belonged to three groups: (1) C. aronia var. arona accessions; (2) C. aronia var. dentata accessions; and, (3) C. monogyna subsp. azarella and C. orientalis var. orientalis accessions. The principle component analysis results also revealed that the first three components explained 46, 21, and 14% of the variation, comprising a total of 81%. The fruit length and width, leaf area, and soluble solids contents were highly correlated characteristics for the first three components. The 19 RAPD primers generated a total of 107 bands, where 76 of these were polymorphic. The molecular data analyses by principle coordinate and clustering showed similar results to those of pomological characteristics. There were three groups, (1) C. aronia var. arona accessions; (2) C. aronia var. dentata accession; and, (3) C. monogyna subsp. azarella. C. orientalis var. orientalis accession grouped with C. aronia var. arona accessions. Therefore, it can be concluded that, overall, the diversity patterns of pomological and molecular data, generated by RAPD, for Crataegus are in good agreement and the accessions of C. aronia var. aronia, C. aronia var. minuta, C. monogyna subsp. azarella and C. orientalis var. orientalis accessions.