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Öğe 45S5 Bioactive Glass-Ointment Positively Effects on Wound Healing in Rats by Regulating TNF?, Il-10, VEGF, and TGF?(Wiley, 2024) Kirgiz, Omer; Altug, Muhammed Enes; Ozkan, Huseyin; Han, Mehmet Cengiz; Akcakavak, Goekhan; Ozarslan, Ali Can; Yucel, SevilAimThis study aimed to investigate the effects of 45S5 bioactive glass-ointment (BG) on cutaneous wound healing in rats at the molecular, biochemical, and histopathological levels.Materials and MethodsThirty-two rats were divided into four groups (n = 8): Control, Sham, BG, and DEX (Dexpanthenol). While no wound treatment was applied to the CONTROL, a wound model was created in the Sham, and no treatment was applied. A wound model was created for other groups, and BG and DEX were applied locally for 21 days. During the 21-day experiment period, feed and water consumption and weight changes were observed. Wound areas were calculated on days 0, 3, 7, 4, and 21. Following treatment, the rats were euthanized and tissues from the wound area and blood samples were collected. While the expression levels of tumor necrosis factor-alpha (TNF alpha), Interleukin 6 (IL6), Interleukin 10 (IL10), transforming growth factor-beta (TGF beta), and vascular endothelial growth factor (VEGF) genes were determined by qPCR, the levels of TNF alpha, IL6, and IL10 proteins were measured by ELISA.ResultsIt was observed that the BG group showed anti-inflammatory activity by suppressing TNF alpha levels and stimulating IL-10. In addition, it was determined that BG increased fibroblast activity and vascularization.ConclusionCurrent findings showed that topical application of BG has anti-inflammatory effects, while also accelerating healing by increasing vascularity and making positive contributions to tissue healing. This study investigates the effects of topical application of 45S5 bioactive glass-ointment (BG) on wound healing in rats at the molecular and histopathological levels. The findings demonstrate that BG exhibits anti-inflammatory activity, enhances vascularization, accelerates healing, and contributes positively to tissue repair.imageÖğe Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze-thawed ram spermatozoa(Wiley, 2021) Bucak, Mustafa Numan; Akalin, Pinar Peker; Keskin, Nazan; Bodu, Mustafa; Ozturk, Ali Erdem; Ili, Pinar; Ozkan, HuseyinBackground: Freeze-thawing process negatively affects ram spermatozoa in terms of sperm quality, DNA integrity and antioxidant defence system. Thus, antioxidant supplementation of spermatozoa during freeze-thawing is suggested to improve sperm parameters. Objectives: The aim of this study was to determine the effects of fetuin and trehalose added into ram semen extender on sperm parameters, antioxidant parameters, antioxidant-related gene expressions and DNA integrity during the freeze-thawing process, in low glycerol concentration. Methods: Semen samples collected from six mature rams were pooled and splitted into equal aliquots and diluted with a tris-based extender containing different concentrations of glycerol (G5; %5 and G3; %3), fetuin (F; 2.5, 5 and 15 mg/mL) and trehalose (60 mm) as eight groups (G5F0, G5F2.5, G5F5, G5F15, G3F0, G3F2.5, G3F5 and G3F15). Results: G3F5 group resulted in the highest motility, mitochondrial activity and viability and the lowest DNA fragmentation and DNA damage (p < 0.05). Also, G3F0 displayed considerably more cryoprotective effect compared with G5F0 group (p < 0.05) in terms of motility, mitochondrial activity and viability rates. Lipid peroxidation levels decreased in G5F5 group compared with G5F0 group (p < 0.05). The levels of total glutathione increased in G3F2.5 group (p < 0.05) in comparison with the G5F0 group. NQO1 gene levels were upregulated approximately twofold in G5F5, G5F15, G3F2.5, G3F5 and G3F15 groups compared with G5F0 group (p < 0.05). The levels of GCLC gene were approximately twofold higher in G3F0, G3F2.5, G3F5 and G3F15 groups compared with G5F0 group (p < 0.05). GSTP1 gene levels were significantly higher with different levels in all treatment groups except for G5F2.5 and G3F0 groups in comparison with G5F0 group (p < 0.05). Conclusions: Co-supplementation of tris-based extender having low glycerol (3%) with trehalose and fetuin to enhance the quality of ram spermatozoa after freeze-thawing process is recommended.Öğe Comparative evaluation of major milk quality parameters of Holstein and Simmental cows at different lactation stages under similar environmental conditions(Polish Soc Veterinary Sciences Editorial Office, 2023) Kaya, Ufuk; Ozkan, Huseyin; Yazlik, Murat Onur; Camdeviren, Baran; Gungor, Guven; Karaaslan, Irem; Dalkiran, SevdaThe aim of this study was to evaluate the composition and quality parameters of Simmental and Holstein cows' milk with different lactation stages under the same environmental conditions. Multiparous Holstein and Simmental cows from different lactation stages (n = 210) were included in the present study. MDA, SCC, composition, and fatty acid analyzes were performed from the collected milk samples. To determine the effect of breed, lactation stage and their interactions, linear mixed models were applied to these parameters. Among the breeds, only milk fat and pH were determined statistically significant as composition parameters. While C15:0 and C17:1 n8 were observed to be statistically significant for the breed factor, C18:2 n6 trans were statistically significant only for the lactation stage factor. Moreover, the fatty acids of C4:0, C6:0, C8:0, C10:0, C11:0, C12:0, C13:0, C16:0, C18:1 n9, C20:0 and C22:6 n3 were determined to be statistically significant in terms of interactions. For the fatty acid indices, SCFA, MCFA, n3 and n6/n3 were statistically significant in terms of interactions while SFA, MUFA, UFA and AI were statistically significant in terms of breeds. On the other hand, new studies are needed to investigate the differences between these breeds at the molecular level for milk quality and fatty acid synthesis.Öğe Comparative Evaluation of RNAlater Solution and Snap Frozen Methods for Gene Expression Studies in Different Tissues(Sciendo, 2020) Ozkan, Huseyin; Kerman, EnesIntroduction: Freezing of tissues with liquid nitrogen is the most common method in studies performed at the RNA level. However, the use of RNA stabilization solutions has become a popular alternative method. The aim of this study is to investigate the effectiveness of RNAlater on RNA stabilization in different tissues. Material and Methods: In this study, RNA were isolated from the lung, heart, liver and skeletal muscle tissues of rats that were frozen with liquid nitrogen (snap frozen, SF group) or stored in RNAlater solution (RL group), and the changes in concentration, purity, reference genes expression, and fold-change levels between groups were analyzed. Results: In the RL group, the concentration of RNA isolated from the liver tissues was higher (P<0.05), whereas the A260/280 ratio was lower in the heart and liver tissues (P<0.05). PPIA and SRP72 genes were found to have lower Cl values in the heart tissues ofrats in the RL group (P<0.05 and P<0.001, respectively) than the SF group. Expression levels of PPIA, ACTB, and SRP72 genes across the tissues were found to be different between the groups (P<0. 05). The gene expression level examined in terms of fold-change was significantly different in the RL group (upregulated up to 4 folds and downregulated about 0.5 fold) (P< 0.05). Conclusions: The results showed that RNAlater can maintain the RNA integrity and can also change the results of gene expression because it does not inhibit biological activity. The snap freezing method is more reliable because gene expression is more stable in tissues frozen with liquid nitrogen.Öğe Considering potential roles of selected MicroRNAs in evaluating subclinical mastitis and Milk quality in California mastitis test (+) and infected bovine milk(Wiley, 2024) Ozkan, Huseyin; Keceli, Hasan Huseyin; Kaya, Ufuk; Dalkiran, Sevda; Yuksel, Murat; Tek, Erhan; Yakan, AkinThis study investigates the relationships between subclinical mastitis and milk quality with selected microRNAs in cow milk. California Mastitis Test (CMT)-positive (n = 20) and negative (n = 20) samples were compared (Experiment I). Additionally, samples with CMT-positive but microbiological-negative, as well as positive for only Staphylococcus subspecies (Staph spp.) and only Streptococcus subspecies (Strep spp.) were examined (Experiment II). Four groups were formed in Experiment II: Group I (CMT and microbiological-negative) (n = 20), Group II (CMT-positive but microbiological-negative) (n = 10), Group III (Staph spp.) (n = 5), Group IV (Strep spp.) (n = 5). While electrical conductivity, somatic cell count (SCC), malondialdehyde (MDA) increased, miR-27a-3p and miR-223 upregulated and miR-125b downregulated in the CMT-positive group in Experiment I. SCC and MDA were higher in CMT-positive groups. miR-27a-3p and miR-223 upregulated in Groups III and IV. While miR-155 is upregulated, miR-125b downregulated in Group IV. Milk fat is positively correlated with miR-148a and miR-223. As miR-27a-3p positively correlated with SCC and MDA, miR-125b negatively correlated with electrical conductivity and SCC. miR-148a and MDA were positively correlated. miR-155 was correlated with fat-free dry matter, protein, lactose, and freezing point. miR-223 was positively correlated with SCC and miR-148a. Results particularly highlight miR-27a-3p and miR-223 as potential biomarkers in subclinical mastitis, especially those caused by Staph spp. and Strep spp., while miR-148a, miR-155, and miR-223 stand out in determining milk quality.Öğe Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses(Academic Press Inc Elsevier Science, 2020) Bucak, Mustafa Numan; Keskin, Nazan; Ili, Pinar; Bodu, Mustafa; Akalin, Pinar Peker; Ozturk, Ali Erdem; Ozkan, HuseyinThis study aimed to evaluate the comparative effects of taxifolin hydrate and trehalose on the quality of frozen-thawed ram spermatozoa for the first time. Ejaculates collected from six mature rams were pooled, and divided to eight equal aliquots to extend them with different concentrations of glycerol (%5 and %3), taxifolin hydrate (10, 100, and 500 mu M), and trehalose (60 mM) as eight groups (G5T0, G5T10, G5T100, G5T500, G3T0, G3T10, G3T100, and G3T500). After freeze-thawing process of cryopreservation, microscopic and oxidative stress parameters, and gene expression levels were investigated for understanding of possible impacts of taxifolin hydrate and trehalose. The study showed that G3T10 resulted in the highest post-thawed viability and mitochondrial activity. Moreover, all extenders with taxifolin hydrate reduced DNA fragmentation in comparison to G5T0, but DNA damage was prevented at the highest rate in presence of G5T10. The level of LPO significantly decreased in the groups G5T500 and G3T100, and the expression levels of NQO1, GCLC, and GSTP1 genes significantly increased in the groups G5T100, G5T500, G3T10, and G3T100 compared to the group G5T0. Finally, co-supplementation of tris-based extender having 3% glycerol with 60 mM trehalose and 10 mu M taxifolin hydrate in cryopreservation extender may be recommended to improve the quality of post-thawed ram spermatozoa. However, further in vivo and in vitro studies are suggested to evaluate fertility rates of frozen-thawed ram spermatozoa co-supplemented with trehalose and taxifolin hydrate.Öğe Determination of expression patterns of miR-26a, and preimplantation factor levels for early pregnancy detection in nulliparous and multiparous cows(Wiley, 2024) Olgac, Kemal Tuna; Yazlik, Murat Onur; Ozkan, Huseyin; Kaya, Ufuk; Tirpan, Mehmet BorgaFor maximum productivity in a dairy farm, the earliest and the most accurate detection of pregnancy is essential. The aim of this study was to determine the efficacy of expression patterns of miR-26a, and serum Preimplantation Factor (PIF) levels for pregnancy diagnosis during the early pregnancy in nulliparous and multiparous cows. A total of 60 cows (30 nulliparous and 30 multiparous Holstein cows) were enrolled in the study. Blood samples were collected for miR-26a on days 8 and 16 (D8 and D16), and for the PIF on days 10 and 20 (D10 and D20) following insemination (D0). Pregnancies were determined by ultrasonography on the 28th day after insemination. Expression levels of miR-26a determined by qPCR. PIF levels were assessed by using commercial ELISA kits. All data were analyzed by using the MIXED procedure of SPSS. The expression levels of miR-26a were 6.64 folds higher on D16 in pregnant compared to non-pregnant multiparous cows (p < .05). On D8 and D16, miR-26a expression levels were found higher 13 folds in pregnant compared to non-pregnant nulliparous cows (p < .05). Additionally, miR-26a expressions were higher 5.42 folds (p < .05) on D8, 7.19 folds higher (p < .01) on D16 in pregnant nulliparous and multiparous cows, and were 6.30 folds higher (p < .001) on D8 and D16 according to non-pregnant animals. PIF levels were greater in pregnant animals (p < .05). Analyzing miR-26a on D8 might be considered as sufficient in nulliparous cows. Pregnancy detection in multiparous cows can be made on the 16th day with this method. Furthermore, PIF evaluations may be sufficient on D10 in multiparous cows. Besides, PIF levels and miR-26a expression levels might be used safely in field conditions and clinical applications.Öğe Diagnostic accuracy of milk components for pregnancy diagnosis in mid and late lactation cows(Croatian Dairy Union, 2023) Kaya, Ufuk; Yazlik, Murat Onur; Ozkan, Huseyin; Camdeviren, Baran; Gungor, Guven; Dalkiran, Sevda; Keceli, Hasan HuseyinThe aims of this study were to establish a cut-off point by evaluating the usability of the somatic cell count (SCC) and milk components (fat, fat-free dry matter (FFDM), protein, lactose, freezing point, electrical conductivity and pH) to observe the pregnancy status, and to determine the practical usage of these parameters as diagnostic biomarker of pregnancy status. In the present study, primiparous Holstein cows (n=133) were included in the mid and late lactation. Milk samples were collected in sterile tubes for SCC and milk components analysis. In each lactation period, SCC, milk yield and milk component parameters were analysed by Student's t test according to pregnancy status. Receiver operating characteristic curves were used to determine the predictive threshold using SCC and milk component parameters to discriminate between pregnant and non-pregnant cows. SCC levels were similar for all cows in the mid and late-lactation. In the mid lactation, FFDM, protein, lactose and electrical conductivity were higher and milk yield, fat, freezing point and pH were lower in pregnant cows (p<0.05). In the late lactation, FFDM, protein, lactose and electrical conductivity were significantly higher and milk yield, fat and pH were significantly lower in pregnant pH were the best predictors for pregnancy diagnosis in mid-lactating cows with the AUC values of 0.840, 0.768, 0.780, 0.772, 0.693, 0.792, and 0.901 respectively. Furthermore, fat, FFDM, protein, lactose, electrical conductivity, and pH could be useful diagnostic tools for pregnancy determination in late lactating cows with the AUC values of 0.869, 0.684, 0.661, 0.689, 0.756, and 0.841 respectively. In conclusion, the milk components could be used as rapid, easily accessible, and inexpensive markers for the evaluation of the diagnosis of pregnancy status in primiparous Holstein cows.Öğe Dose-dependent effects of simvastatin, atorvastatin and rosuvastatin on apoptosis and inflammation pathways on cancerous lung cells(Ankara Univ, 2023) Dikmen, Nursel; Ozkan, Huseyin; Cimen Acikgul, Funda; Camdeviren, Baran; Ay, Emrah; Ambarcioglu, Pinar; Duran, NizamiThe aim of study was to investigate the anti-proliferative and inflammatory effects of atorvastatin, rosuvastatin, and simvastatin in lung cancer. The effects of statins were investigated in Vero, BEAS-2B, and A549 cell lines. In addition to expressions of BAX, BCL-2, TNF alpha, IL-10, IL-6, protein levels of TNF alpha, IL-10, IL-6 were determined. Cell viability and MDA were also measured. While the cell numbers in groups with low doses of statins were found to be approximately 1x106/mL, proliferation was inhibited at higher rates containing high doses. Simvastatin, rosuvastatin, and high dose atorvastatin upregulated the BAX, while high dose of atorvastatin and both doses of rosuvastatin caused downregulation in BCL-2. All statin groups had higher MDA. Simvastatin and high dose rosuvastatin upregulated TNF alpha. While low dose simvastatin and atorvastatin and high dose atorvastatin and rosuvastatin upregulated IL-10, IL6 was upregulated with a low dose of rosuvastatin. TNF alpha was higher in simvastatin and rosuvastatin groups. IL-10 was highest in rosuvastatin groups. Atorvastatin groups had lower IL-6. Although cell numbers have been reduced by all statins, rosuvastatin is more effective on studied genes.Öğe Effect of essential oil supplementation to diet on meat quality, fatty acid composition, performance parameters and intestinal microbiota of Japanese quails(Wiley, 2021) Kurekci, Cemil; Ozsoy, Bulent; Hassan, Errol; Ozkan, Huseyin; Gundogdu, Aycan; Ozsoy, Sule Yurdagul; Yakan, AkinThe effect of essential oil (EO) supplementation on carcass characteristics of Japanese quails and interactions between ingredients and intestinal morphology were investigated in this study. A total of 250 quails were fed different diet: D1, basal diet (BD); D2, BD plus palmarosa oil (PO; 100 mu g/kg diet); D3, BD plus lemon myrtle oil (LMO; 100 mu g/kg diet); D4, BD plus alpha-Tops (mixture of alpha-terpineol, cineole and terpinene-4-ol; 100 mu g/kg diet); and D5, BD plus cyclodextrin. Overall growth performance was determined at multiple time points during 35 days of experiment. Carcass characteristics (fatty acid, pH and colour), intestinal morphology and the expression levels of meat quality-related genes including the insulin-like growth factor (IGF-1), myogenin and avian uncoupling protein (avUCP) were examined at the end of the trial. Additionally, intestinal microbiome of quails was studied by next-generation sequencing-based culture-independent analysis. Although the inclusion of EOs into the diet had no effect on the growth performance of quails and the microbial profile, the significant changes in pH(24)and colour (a*) of the quail's breast muscle (p < .05) in the group receiving PO were observed. Additionally, oleic acid content in the breast muscle was significantly higher in the EOs supplemented groups (p < .01). Quails fed the PO supplemented diet had higher villus and relatively rich in oleic acid. The expression levels of IGF-1 and myogenin genes in quail's muscle were not affected, but the expression of avUCP gene was significantly lower in quails fed with LMO and alpha-Tops (p < .05). The results demonstrated variable effects of these treatments on intestinal morphology. Taken together, dietary inclusion of EOs is found to be beneficial and hence can be recommended for improving the quality of poultry meat.Öğe Effects of Oxidative Stress-Related Major Molecular Parameters on Milk Composition in Weaning Period of Damascus Goats [1](Kafkas Univ, Veteriner Fakultesi Dergisi, 2022) Ozkan, Huseyin; Dalkiran, Sevda; Karaaslan, Irem; Kaya, Ufuk; Camdeviren, Baran; Yuksel, Murat; Yakan, AkinThe weaning process in goat breeding is applied in various methods depending on the breeding administration. In this study, blood and milk samples have been collected from Damascus goats at the weaning day (postpartum 105th day-weaned day) and a week later (post-weaned day). In addition to determining cortisol and MDA in plasma, COX-2 and NFE2L2 activities have been investigated both mRNA and protein levels from leucocytes and plasma. Compositional parameters of milk have also been analyzed and the possible relations between studied parameters have been investigated. While expression levels of COX-2 and SCC of milk decreased, pH of milk was increased in post-weaned day samples. The MDA, FFDM, protein, lactose and freezing point were decreased in the post-weaned day. Milk fat was negatively correlated with NFE2L2, and milk protein had positively correlated with SCC and FFDM. On the other hand, lactose was positively correlated with FFDM and protein. In addition, most of the compositional parameters positively correlated with a freezing point; they were negatively correlated with electrical conductivity. According to the results obtained from the study, it is thought that the decrease in milk fat in goats may be an indicator of increased oxidative stress in lactating goats due to the increase in NFE2L2 protein, which has a central role in the antioxidant response.Öğe Effects of propylene glycol used at different doses in Akkaraman lambs rations on metabolism-related parameters and liver gene and protein expression during different feeding periods(Wiley, 2023) Yakan, Akin; Ozkan, Huseyin; Kaya, Ufuk; Keceli, Hasan Huseyin; Dalkiran, Sevda; Karaaslan, Irem; Unal, NecmettinThis study aimed to investigate the metabolic effects of propylene glycol (PG) over 60, 90, and 120 days in lambs. Seventy-two weaned male lambs were allocated into three groups: control (Con), PG1.5 (1.5 mL/kg live weight0.75), and PG3 (3 mL/kg live weight0.75). Blood samples were collected at the beginning and slaughter days. Biochemical parameters (glucose, triglycerides, ALT, AST, LDH, BUN, and insulin) and gene and protein levels of peroxisome proliferator activated receptor gamma (PPAR gamma), diacylglycerol o-acyltransferase 1 (DGAT1), carbohydrate responsive element binding protein (ChREBP), and sterol regulatory element binding transcription factor 1c (SREBP-1c) in the liver were determined. Glucose in PG1.5 was increased on Day 60, while significant differences were observed in biochemical parameters except for insulin on the 60, 90, and 120 days. Biochemical parameters such as ALT, AST, LDH, and BUN increased over time, while triglycerides decreased. DGAT1 gene and protein levels were lower, while SREBP-1c and PPAR gamma were higher in PG groups on Day 60. While SREBP-1c was lower in PG1.5, ChREBP was higher in PG3 on Day 90. PPAR gamma, DGAT1, and ChREBP were upregulated in PG3 on Day 120. Positive correlations were found between proteins. The long-term use of PG in lambs did not have detrimental effects on metabolism. The study provides valuable insights into the molecular mechanisms underlying the metabolic effects of PG in lambs, shedding light on its potential applications in lamb production.Öğe The effects of the feeding duration of propylene glycol on major meat quality parameters and substantial proteins in the muscle of Akkaraman lambs(Elsevier Sci Ltd, 2024) Yakan, Akin; Ozkan, Huseyin; Kaya, Ufuk; Keceli, Hasan Huseyin; Karaaslan, Irem; Dalkiran, Sevda; Akcay, AytacIn this study, the effects of propylene glycol (PG) on meat quality and molecular pathways related to energy metabolism in longissimus lumborum muscle on lambs were evaluated. Seventy-two lambs were divided into three groups consisting of 60th, 90th, and 120th of slaughter days. The dosage of the PG and slaughter days were the variables used in the study. Eight animals were slaughtered from each group on each day. The meat quality parameters (e.g., pH, protein, fatty acid profile) and IGF-1, IGFBP4, and DGAT1 (i.e., mRNA and protein levels) were evaluated. The pH 45 min post-slaughter was higher in PG groups on 120th day. On the 4th day after slaughter, the b value was the lowest in the PG3, while 7th day after slaughter it was highest in Con and PG3 on 90th day. The total n3 and n6 were lowest and the NV was highest on 120th day. The IGFBP4 was upregulated in the PG groups on all of the slaughter days. The DGAT1 was upregulated in the PG3 on the 90th day. The IGF-1, DGAT1, IGFBP4 protein levels were found to have increased in the PG3 on 90th day. The IGFBP4 was found to have decreased in the PG3 on 120th day. According to the results of the study, the oral administration of the PG at the 3 mL/kg live weight0.75 for at least 120 days may have positive effects on meat quality in lambs through the IGF-1, DGAT1, and IGFBP4 genes and the proteins encoded by these genes.Öğe Expression patterns of inflammatory and oxidative stress-related genes in the uterine and ovarian tissues of dogs diagnosed with pyometra based on cervical patency status(Elsevier Science Inc, 2024) Ozkan, Huseyin; Yazlik, Murat Onur; Keceli, Hasan Huseyin; Vural, Sevil Atalay; Mustak, Inci Basak; Kaya, Ufuk; Altinbas, Yunus FurkanThis study aimed to investigate the expression patterns of genes associated with inflammation and oxidative stress in ovarian and uterine tissues of dogs with pyometra, categorized by cervical status (open cervix or closed cervix), which influences disease severity. The control group comprised healthy animals undergoing elective ovariohysterectomy. Tissue inflammatory gene expression and Malondialdehyde (MDA) levels were determined while microbial and histopathological examinations were conducted, along with immunohistochemical evaluations. In the closed-cervix group, uterine TNF and IL6 were upregulated approximately 10- fold while IL10 was upregulated nearly 5-fold. TNF expression differed remarkably between the pyometra groups. In the closed-cervix group, PTGS2 and HMOX1 were upregulated approximately 5-fold whereas NFE2L2 expression was downregulated. The closed-cervix group also had the highest uterine MDA levels. Regarding ovarian tissue, MDA levels were higher in the closed-cervix group than in the open-cervix group while IL10 expression was lower in the closed-cervix group than the open-cervix group. In the closed-cervix group, NFE2L2 was downregulated whereas HMOX1 was upregulated. Uterine TNF levels were positively correlated with IL6, , IL10, , PTGS2, , and HMOX1, , but negatively correlated with NFE2L2. . IL6 was positively correlated with IL10, , PTGS2, , and HMOX1. . NFE2L2 was negatively correlated with IL6 and HMOX1. . IL10 was positively correlated with PTGS2 and HMOX1. . MDA was positively correlated with TNF, , IL6, , IL10, , PTGS2, , NFE2L2, , and HMOX1. . TNF levels were positively correlated with ovarian PTGS2, , and with IL6 and NFE2L2. . MDA was positively correlated with PTGS2 and HMOX1. . MDA could be an important biomarker for understanding the severity of pyometra. Moreover, TNF expression and its relationships with various studied parameters such as IL10 may contribute to treatment and prognostic biomarker studies in closed-cervix pyometra pathology.Öğe Expression patterns of major genes in fatty acid synthesis, inflammation, oxidative stress pathways from colostrum to milk in Damascus goats(Nature Portfolio, 2021) Yakan, Akin; Ozkan, Huseyin; Camdeviren, Baran; Kaya, Ufuk; Karaaslan, Irem; Dalkiran, SevdaThe molecular regulation of milk secretion and quality in the transition period from colostrum to milk in goats is largely unknown. In the present study, mammary gland secretion of goats was collected in 0th, 4th, 7th, 14th and 28th days after parturition. In addition to composition and fatty acid profile of colostrum or milk, FASN, SCD, ACACA, COX-2, NRF2, TLR2, NF-kB, LTF and PTX3 genes expression patterns were determined from milk somatic cells. While somatic cell count (SCC), malondialdehyde (MDA), fat, fat-free dry matter, protein and lactose were highest as expression levels of the oxidative and inflammatory genes, freezing point and electrical conductivity were lowest in colostrum. With the continuation of lactation, most of the fatty acids, n3 ratio, and odour index increased but C14:0 and C16:0 decreased. While FASN was upregulated almost threefolds in 14th day, ACACA was upregulated more than fivefolds in 7th and 14th days. Separately, the major genes in fatty acid synthesis, inflammation and oxidative stress were significantly associated with each other due to being positively correlated. MDA was positively correlated with SCC and some of the genes related inflammation and oxidative stress. Furthermore, significant negative correlations were determined between SCC and fatty acid synthesis related genes. With this study, transition period of mammary secretion was particularly clarified at the molecular levels in Damascus goats.Öğe The levels of milk fatty acids and alterations of correlations between them in weaning process in damascus goats(Sivar-Soc Italiana Veterinari Animali Reddito, 2022) Ozkan, Huseyin; Karaaslan, Irem; Kaya, Ufuk; Dalkiran, Sevda; Yuksel, Murat; Yakan, AkinWeaning in goat breeding is applied with varying periods and practices. During lactation stages, there are notable changes in the fatty acid profile of goat milk. Weaning is one of the potential breeding practices that may affects the milk fatty acid profile in goats. The aim of this study was to determine the changes in fatty acid profile of milk during weaning process in Damascus goats. Possible changes in the relationships between milk fatty acids and Somatic Cell Score (SCS) were also investigated in the study. Milk samples were collected during morning milking from 24 healthy Damascus goats in the weaned day ( WD) and one week after the weaning (Post Weaned Day, PWD). SCS and fatty acid profile of collected milk samples in both sampled days were determined. Nutritive value, odour and atherogenic indexes of milk samples were also evaluated. While SCS was dramatically decreased after weaning, significant changes at varying levels were determined in milk fatty acids. The levels of C10:0, C12:0, C16:0, and C20:5 n3 fatty acids were lower in the PWD samples than the WD samples, while C11:0, C16:1 n7, C17:0, C17:1 n8, C18:1 n9, C20:0, C20:3 n6, C21:0, C23:0, and C24:0 fatty acids were found at higher levels. Compared to WD samples, Long-chained Fatty Acids (LCFA), Unsaturated Fatty Acids (UFA), Mono-Unsaturated Fatty Acids (MUFA), and Nutritive value parameters were higher in PWD samples. On the other hand, Medium-Chained Fatty Acids (MCFA), Saturated-Fatty Acids (SFA), and atherogenic index were found lower in PWD samples than WD samples. Furthermore, it was determined that the correlations between the fatty acids in milk were changed after weaning application. It was determined that the weaning process had considerable effects on the milk fatty acid profile, and it was thought that the milk quality of Damascus goats increased significantly after weaning application.Öğe The Matrix Metalloproteinase Inhibitor Batimastat Reduces Epidural Fibrosis After Laminectomy in Rats(Turkish Neurosurgical Soc, 2023) Yurtal, Ziya; Ozkan, Huseyin; Kutlu, Tuncer; Deveci, Mehmet Zeki Yilmaz; Urfali, Boran; Urfali, SenemAIM: To investigate the efficacy of locally applied batimastat after laminectomy in preventing postoperative epidural fibrosis. MATERIAL and METHODS: Thirty-two Wistar albino male rats weighing 200-250 g were used. The rats were assigned to four different groups (I-Control group, II-sham group, III-Laminectomy+Batimastat group, and IV-Laminectomy+Spongostan (TM) group). The rats were euthanized 28 days after surgery before TNF-alpha, IL6, IL-1 beta, IL10, TGF-beta 1, and MMP9 gene expression levels of tissue in the surgical area were determined with qPCR. TNF-alpha, IL6, and IL10 protein levels were also measured in both tissue and plasma. In addition, the surgical area was evaluated by histopathological and immunohistochemical methods. RESULTS: TNF-alpha, IL6, and IL-1 beta gene expression levels were higher in the batimastat group than in the control group. Whereas IL10 gene expression levels increased about two-fold in the sham and Spongostan (TM) groups, in the batimastat group, it was similar to that in the control group. TGF-beta 1 gene expression was three-fold higher in the sham group but was similar to that in the control group in both batimastat and Spongostan (TM) groups. MMP9 gene expression levels significantly decreased only in the batimastat group. In addition, fibrosis score, fibroblast cell count, inflammatory cell count, and CD105 expression decreased in the batimastat group relative to the control. CONCLUSION: Molecular and pathological examination results suggested that batimastat is an effective agent in reducing the occurrence of epidural fibrosis after laminectomy.Öğe Molecular, biochemical, and histopathological effects of long-term low and high-percentage fructose consumption on the liver in rats(Ankara Univ Press, 2022) Ozkan, Huseyin; Kutlu, Tuncer; Yakin, Akin; Ozsoy, Sule YurdagulThe aim of this study was to investigate the lipogenic and inflammatory effects of low and high percentage fructose solutions in rats. Wistar albino rats were fed with fructose solutions for 10 weeks. The groups were as follows: Cont (Control), F15 (Fructose 15%), F30 (Fructose 30%), and F60 (Fructose 60%). Rats' body weights were measured weekly. Also, lipogenic and inflammatory gene expression levels, biochemical parameters, and histopathological changes in the liver were investigated. After 10 weeks, it was observed that the animals in the F60 were the heaviest, while the animals in the F30 were the lightest. In all experimental groups, triglycerides were significantly higher than those of controls (P<0.05). In F30 and F60, TNF alpha, IL-6, and IL-1/1 were upregulated in the liver compared to control (P<0.05). In addition, SREBP-1c, ChREBP, FAS, ACACA, and SCD-1 were upregulated in all fructose feeding groups compared to Cont (P<0.05). The livers of rats in the F30 and F60 groups had degenerative changes and steatosis. The most detrimental effects of fructose were observed in F60. The concentration of fructose was found to be a very important factor for maintaining normal liver physiology at the molecular level.Öğe Nobiletin attenuates acetaminophen-induced hepatorenal toxicity in rats(Wiley, 2020) Guvenc, Mehmet; Cellat, Mustafa; Gokcek, Ishak; Ozkan, Huseyin; Arkali, Gozde; Yakan, Akin; Ozsoy, Sule YurdagulThe study aimed to examine the effects of nobiletin on the toxicity model induced with acetaminophen (APAP). For this purpose, 24 adult male rats were equally divided into four groups. The groups were the control group (group 1); dimethyl sulfoxide only, the APAP group (group 2) received a single dose of APAP 1000 mg/kg on the 10th day of experiment; the Nobiletin group (group 3), nobiletin (10 mg/kg) for 10 days; and the APAP + Nobiletin group (group 4), nobiletin (10 mg/kg) for 10 days with a single dose of APAP (1000 mg/kg) administered on the 10th day and the experiment ended after 48 hours. At the end of the study, a significant increase in malondialdehyde, interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) levels and a significant decrease in glutathione levels, glutathione peroxidase activities and nuclear factor erythroid-derived 2-like 2 (Nrf-2) and heme oxygenase-1 (HO-1) expressions were observed with APAP application in liver and kidney tissues. Serum aspartate transaminase (AST), alanine transaminase (ALT), urea, and creatinine levels were also significantly increased in the APAP group. However, nobiletin treatment in group 4 reversed oxidative stress and inflammatory and histopathological signs caused by APAP. It is concluded that nobiletin may be a beneficial substance that confers hepatorenal protection to APAP-induced toxicity via antioxidant and anti-inflammatory mechanisms.Öğe A novel approach for evaluating of goat milk quality: canonical correlation analysis between major milk composition parameters and fatty acid components in Damascus goats(Sivar-Soc Italiana Veterinari Animali Reddito, 2021) Kaya, Ufuk; Ozkan, Huseyin; Camdeviren, Baran; Karaaslan, Irem; Akin, YakanThe aim of this study was to investigate the effects of major milk composition parameters (lactose, protein and fat) and somatic cell score (SCS) on the variation of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), unsaturated fatty acids (UFA), omega 6 (omega 6), omega 3 (omega 3), odour index (OI) and nutritive value (NV) of goat milk in the first month of the lactation. For this aim, 120 milk samples were collected in different times post-partum (0, 4, 7, 14 and 28 days) from 24 Damascus goats. Composition parameters and fatty acid components of milk samples were determined. Pearson correlation coefficient and canonical correlation analysis (CCA) were used to evaluate the relationships between major milk parameters and fatty acids components. In addition to positive correlations between major milk parameters, significant and variable correlations were found between major milk composition parameters and fatty acid components according to the Pearson Correlation coefficient. Most of the fatty acids components were also correlated with each other. However, only the first of four calculated pairs of canonical variables was found to be significant, strongly (r(c)= 0.857, P< 0.001). According to the calculations of canonical loading and cross-loading results, protein and lactose from the set of independent variables and OI and NV from the set of dependent variables were found to be the most important variables. On the other hand, redundancy indexes results showed that the fatty acid contents may be explained by the milk components in goat milk. CCA results has revealed that multidimensional evaluation of measurements in dependent and independent variable sets can provide to researchers with important and innovative information for assessment of milk quality. Considering the multiple relationships with a multivariate approach, strong and significant relations were found for the first time in terms of major milk quality parameters in goats.
