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Öğe Effect of essential oil supplementation to diet on meat quality, fatty acid composition, performance parameters and intestinal microbiota of Japanese quails(Wiley, 2021) Kurekci, Cemil; Ozsoy, Bulent; Hassan, Errol; Ozkan, Huseyin; Gundogdu, Aycan; Ozsoy, Sule Yurdagul; Yakan, AkinThe effect of essential oil (EO) supplementation on carcass characteristics of Japanese quails and interactions between ingredients and intestinal morphology were investigated in this study. A total of 250 quails were fed different diet: D1, basal diet (BD); D2, BD plus palmarosa oil (PO; 100 mu g/kg diet); D3, BD plus lemon myrtle oil (LMO; 100 mu g/kg diet); D4, BD plus alpha-Tops (mixture of alpha-terpineol, cineole and terpinene-4-ol; 100 mu g/kg diet); and D5, BD plus cyclodextrin. Overall growth performance was determined at multiple time points during 35 days of experiment. Carcass characteristics (fatty acid, pH and colour), intestinal morphology and the expression levels of meat quality-related genes including the insulin-like growth factor (IGF-1), myogenin and avian uncoupling protein (avUCP) were examined at the end of the trial. Additionally, intestinal microbiome of quails was studied by next-generation sequencing-based culture-independent analysis. Although the inclusion of EOs into the diet had no effect on the growth performance of quails and the microbial profile, the significant changes in pH(24)and colour (a*) of the quail's breast muscle (p < .05) in the group receiving PO were observed. Additionally, oleic acid content in the breast muscle was significantly higher in the EOs supplemented groups (p < .01). Quails fed the PO supplemented diet had higher villus and relatively rich in oleic acid. The expression levels of IGF-1 and myogenin genes in quail's muscle were not affected, but the expression of avUCP gene was significantly lower in quails fed with LMO and alpha-Tops (p < .05). The results demonstrated variable effects of these treatments on intestinal morphology. Taken together, dietary inclusion of EOs is found to be beneficial and hence can be recommended for improving the quality of poultry meat.Öğe The Effects of Drinking Water Supplemented with Essential Oils on Performance, Egg Quality and Egg Yolk Fatty Acid Composition in Laying Hens(Aves, 2018) Karadagoglu, Ozlem; Ozsoy, Bulent; Olmez, Mukremin; Durna Aydin, Ozlem; Sahin, TarkanThis study was performed to examine the effect of different concentrations of an essential oil mixture (EOM) added to drinking water on growth performance, internal and external egg quality, and egg yolk fatty acid composition in laying hens. A total of 240 Brown Nick laying hens aged 20 weeks were used in this study. These hens were divided into one control and three treatment groups whose drinking water was supplemented with 0, 0.1, 0.2, and 0.3 ml/L of Oregofarm EOM. Oregofarm is a commercially available product containing peppermint, oregano, and anise oil. Each treatment group was further categorized into 10 subgroups, each consisting of 6 laying hens. The hens were fed basal rations for 16 weeks. For data collection and analysis, a 16-week period was divided into three time periods of 5 weeks. The weights of 25- to 30- and 35-week eggs were recorded weekly. In the groups whose drinking water was supplemented with the EOM, there was a significant improvement in egg production (EP) (p<0.001), weekly egg weights during the entire study period (p<0.001), and feed conversion ratio (p<0.05). Furthermore, egg shell thickness and yolk height were significantly increased (p<0.05) at week 35. The Haugh unit was also significantly improved (p<0.05) at weeks 25 and 30. However, feed intake and body weight were not affected by the treatment (p>0.05). The levels of saturated fatty acids were decreased, whereas the levels of polyunsaturated fatty acids increased with EOM supplementation, although the differences were not statistically significant (p>0.05). Therefore, based on the beneficial effects of supplementing drinking water with different concentrations of EOM on egg weight, egg production, feed conversion ratio, and egg yolk fatty acid compositions, it is suggested that EOM can be safely added at concentrations of up to 0.3 ml/L (i.e., the levels used in this study) to drinking water for laying hens.Öğe Effects of glycerol on performance, egg traits, some blood parameters and antibody production to SRBC of laying hens(Elsevier Science Bv, 2010) Yalcin, Sakine; Erol, Handan; Ozsoy, Bulent; Onbasilar, Ilyas; Yalcin, Suzan; Uner, AykutThis study was designed to investigate the effects of the usage of glycerol from biodiesel production from soybean oil in laying hen diets on laying performance, egg traits, heterophils to lymphocytes ratio (H/L), sonic blood parameters and antibody production to SRBC. A total of 180 Lohmann Brown laying hens 39 weeks of age were allocated to four dietary treatments with one control group and three treatment groups and fed for 16 weeks. Each group was divided into five replicates as subgroups, comprising of 9 hens each. Glycerol was used at the level of 2.5, 5 and 7.5% in the diets of the first, second and third treatment groups, respectively. The diets were formulated to be isocaloric and isonitrogenous. Dietary treatments did not significantly affect body weight, egg production, egg weight, feed efficiency, mortality, egg albumen index, egg yolk index and egg Haugh unit, yolk weight percentage, exterior egg quality characteristics, excreta moisture, H/L. ratio, blood parameters and antibody production to SRBC. Hens fed diets with 7.5% glycerol consumed significantly less feed than those of the other groups. Egg yolk cholesterol concentration was significantly higher for hens fed diets with 5 and 7.5% glycerol as compared to those of the other groups (P < 0.01). The ratio of monounsaturated fatty acids (MUFA) to saturated fatty acids (SFA) in eggs was decreased (P < 0.01) with dietary glycerol supplementation. The inclusion of glycerol had no significant effects on blood parameters, H/L ratio, antibody titers to SRBC and excreta moisture. It is concluded that glycerol can be used at 2.5% in the diets of laying hens without adverse effects on the measured parameters. Dietary glycerol at the levels of 5 and 7.5% increased egg yolk cholesterol and decreased the ratio of MUFA/SFA without affecting performance, other egg traits, immune response, H/L ratio, blood parameters and excreta moisture. (C) 2010 Elsevier B.V. All rights reserved.Öğe Effects of inactivated brewer's yeast (saccharomyces cereviciae) on egg production, serum antibody titres and cholesterol levels in laying hens(2013) Sacakli, Pinar; Ergun, Ahmet; Koksal, Bekir Hakan; Ozsoy, Bulent; Cantekin, ZaferThe aim of this study was to investigate the effects of dietary supplementation with inactivated brewer's yeast (Saccharomyces cereviciae) produced from waste of beer industry, on egg production, feed efficiency, serum antibody titers and cholesterol levels in laying hens. A total of 320, twenty two wks old laying hens (Lohmann Brown) were randomly divided into 4 equal groups (each of them 80 hens) with 4 replicates according to the diet regimen; birds were supplemented for 16 wks with 1, 3, 5% Saccharomyces cereviciae whereas in the control group, layers were not supplemented. All diets were formulated to meet or exceed the National Research Council (NRC, 1994) recommended layer requirements for all nutrients and study was carried out for 16 wks. Egg production, feed intakes and feed efficiency were determined biweekly whereas serum antibody titers and cholesterol levels were explored on wks 2 and 16 in 15 birds from each group. At the end of the study, there were no significant effects of yeast supplementation on egg production, feed intake and feed conversion ratio in laying hens whereas there were significant differences (P<0.05) between control and other treatment groups about feed conversion ratio parameters at 28-29th wks of study. Similar to serum antibody titers, cholesterol levels of laying hens also were not altered by yeast supplementation. As a conclusion, Saccharomyces cereviciae had no beneficial effect on production parameters of hens fed with optimal diets and reared under proper management conditions.Öğe Effects of Kefir on Blood Parameters and Intestinal Microflora in Rats: An Experimental Study(Kafkas Univ, Veteriner Fakultesi Dergisi, 2021) Ozsoy, Bulent; Cantekin, Zafer; Yalcin, Sakine; Bayraktar, Hamdullah SuphiA probiotic product of kefir is widely consumed by human beings. The purpose of this research was to investigate the effects of kefir on blood parameters and intestinal flora in rats. A total of 24 female rats were used in this study. During 35 days of experimental period, rats were fed with a commercial diet and water was provided ad libitum. Kefir was given at the levels of 10 mL/kg, 20 mL/kg and 30 mUkg with oral gavage to the first, second and third treatment groups, respectively. Kefir was not given to the control group. The number of yeast was found to be 1.65x10(8) and the number of lactobacilli was found to be 4x10(8) in kefir. At the end of the experiment, blood samples were taken from all rats. Blood plasma parameters and were investigated. The intestinal microflora was investigated by classical colony counting method. No differences were observed among the groups in total protein, albumin, uric acids, SGPT, SGOT, alkaline phosphatase and phosphorus in blood plasma. The plasma triglyceride and cholesterol levels in the second and third groups were lower than control group (P<0.05). No differences were observed in the intestinal pH levels among groups. Although total bacteria number of intestinal microflora was not different in groups, the number of Enterobactericeae and coliform bacteria in the third group was lower than the other groups (P<0.001). The number of Lactobacilli and the yeast level in the intestinal contents were increased by the usage of kefir (P<0.001). It was concluded that positive effects of the kefir were observed in intestinal microflora with increasing the number of beneficial bacteria and decreasing harmful bacteria and therefore kefir has a positive effect on the health of the animals.Öğe The Effects of Lower Supplementation Levels of Organically Complexed Minerals (Zinc, Copper and Manganese) Versus Inorganic Forms on Hematological and Biochemical Parameters in Broilers(Kafkas Univ, Veteriner Fakultesi Dergisi, 2010) Aksu, Devrim Saripinar; Aksu, Taylan; Ozsoy, BulentThe present study was carried out to investigate the effects of replacing inorganic with at lower level of organically complexed minerals (Zn, Cu and Mn) on hematological and biochemical parameters in broilers. A total of two hundred Ross-308 one-day-old broiler chickens were used. Chicks were randomized into 1 control and 3 treated groups each containing 50 chicks and each experimental group comprised 5 of subgroups including 10 chicks. Mineral content of the control diet was prepared according to National Research Council (NRC) as inorganic salts. In treated groups, organically complexed Zn, Cu and Mn were separately added into the basal diet at 1/3 (group 1), 2/3 (group 2) and 3/3 (group 3) proportions as Bioplex(TM), instead of inorganic levels of those minerals recommended by NRC, respectively. The plasma Zn level significantly increased as the serum Cu level significantly decreased (P<0.05) in chickens fed at 2/3 and 3/3 levels of organic minerals. The hemoglobin concentration and packed cell volume were significantly higher in group L1. Total leukocyte count and peripheral blood leukocyte type were in the normal range reported in both the control and organic mineral supplemented groups. As the high density lipoprotein (HDL)-cholesterol level increased, low density lipoprotein (LDL)-cholesterol and total cholesterol levels decreased in chickens fed organically complexed minerals. Results showed that using at much lower level organically complexed minerals (Cu, Zn and Mn) in broiler diets instead of inorganic forms of those minerals has not created a negative impact on hematological and biochemical parameters.Öğe Evaluation of Dietary L-Carnitine Supplementation during the Last Trimester of Pregnancy in Pregnancy Toxemia-Susceptible Goats: An Observational Field Study(Inst Tecnologia Parana, 2024) Urer, Ece Koldas; Kose, Ayse Merve; Raza, Sanan; Saribay, Mustafa Kemal; Dogruer, Gokhan; Ozsoy, BulentL-carnitine, an increasingly vital compound in livestock nutrition, plays a pivotal role in facilitating the beta -oxidation of fatty acids. This study aimed to investigate the impacts of L-carnitine supplementation, administered at varying doses, on pregnancy toxemia susceptible goats. A total of 150 goats underwent estrus synchronization, natural mating, and pregnancy confirmation. Among these, 90 goats carrying multiple fetuses were categorized into three groups on the 100th day of pregnancy, with their feed supplemented as follows: CAR50 (50 mg/kg of L-carnitine), CAR100 (100 mg/kg of L-carnitine), and CON (control without Lcarnitine). Subsequently, blood samples were collected from 15 randomly selected goats from each group on days 100, 115, 130, and 145 of pregnancy to quantify serum levels of beta-HBA (beta-hydroxybutyrate) and NEFA (non-esterified fatty acids), alongside glucose levels. Dietary supplementation of L-carnitine did not exert a significant impact on blood glucose levels in the CAR50 and CAR100 groups, in comparison to the CON group, during the third trimester of pregnancy (P>0.05). Nevertheless, serum NEFA levels exhibited a noteworthy reduction in the CAR50 and CAR100 groups compared to the CON group on day 145 (P<0.01). Furthermore, no substantial fluctuations in mean NEFA and beta-HBA levels were observed in the CAR100 group between days 100 and 145 of gestation (P>0.05). The body condition score exhibited consistent maintenance both within and between groups (P>0.05). To conclude, this study underscores the efficacy of dietary supplementation with L-carnitine in mitigating ketone and NEFA levels in pregnant goats, particularly when administered at a dosage of 100 mg/kg. Consequently, the integration of a standardized quantity of Lcarnitine into the diet holds the potential to serve as a valuable preventive strategy for goats susceptible to pregnancy toxemia.Öğe Investigation of the neuroprotective effect of kefir in experimental spinal cord injury(Ankara Univ, 2023) Yurtal, Ziya; Kutlu, Tuncer; Altug, Muhammed Enes; Ozsoy, Bulent; Alakus, Halil; Yurdagul Ozsoy, SuleIn this study, the antioxidant, anti-inflammatory, and neuroprotective effects of kefir were investigated in spinal cord injury that was experimentally created on rats with a compression trauma model. A total of 56 Wistar-Albino male rats were used in the study. Daily freshly prepared 18 ml/kg/day of kefir was given by oral gavage to animals 7 days before and during the trauma and during the trauma. Spinal cord injury was created according to the weight drop method. On the 1st and 7th days before euthanasia, intracardiac blood was collected for analysis, and then they were sacrificed. The damaged spinal cord segments were examined biochemically, immunohistochemically, and histopathologically. When compared to the sham groups, kefir had a positive effect in the preconditioning and treatment groups by decreasing spinal cord bleeding, edema, myelin sheath damage, liquefactive necrosis, neuronal necrosis, selectivity of canalis centralis, and gitter cell levels significantly. When compared to the sham groups, kefir was found to have a positive effect in the treatment groups by decreasing the neuron specific enolase (NSE), ionized calcium binding adapter molecule 1 (IBA-1), inducible nitric oxide synthase (INOS), cyclooxygenase 2 (COX-2) and myelin basic protein (MBP) levels significantly on the 1st and 7th days, and by increasing the glial fibrillary acidic protein (GFAP) level significantly. As a result, it was demonstrated that kefir had a protective and therapeutic effect on spinal cord injury.Öğe The role of yeast culture (Saccharomyces cerevisiae) on performance, egg yolk fatty acid composition, and fecal microflora of laying hens(Revista Brasileira Zootecnia Brazilian Journal Animal Sci, 2018) Ozsoy, Bulent; Karadagoglu, Ozlem; Yakan, Akin; Onk, Kadir; Celik, Elif; Sahin, TarkanThis study investigated the effects of different levels (0.05, 0.1, and 0.2%) of yeast culture supplementation on body weight, feed intake, feed conversion ratio, egg production, egg weight, egg quality traits, egg yolk fatty acid composition, and microbiological flora in feces. A total of 240 laying hens at 18-19 weeks of age were divided into four groups and fed a basal diet containing 2750 kcal/kg metabolizable energy and 16% crude protein for 16 weeks. The basal diet was supplemented with 0.05, 0.1, and 0.2% commercial yeast culture product obtained from Saccharomyces cerevisiae. The different levels of yeast culture supplementation to the diets did not statistically affect body weight change among the treatments. However, feed intake was lowest in the group fed 0.2% of yeast culture. The highest egg weights were obtained from the groups fed 0.1 and 0.2% yeast culture, when compared with control group. Regarding fatty acid composition, linolenic acid (C18:2 n6) was lowest in the group fed 0.2% yeast culture. However, yeast culture supplementation to the diet did not alter the microbial flora. Yeast culture (S. cerevisiae) supplementation to the diet of laying hens is beneficial for increasing feed intake and egg weight of laying hens without affecting the microbial flora in their digestive system.