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    Acute-Phase Proteins, Oxidative Stress and Enzyme Activities of Blood Serum and Peritoneal Fluid in Cattle with Abomasal Displacement
    (Wiley, 2012) Maden, M.; Ozturk, A. S.; Bulbul, A.; Avci, G. E.; Yazar, E.
    Background Blood serum and peritoneal fluid acute-phase proteins, oxidative stress indicators, and some enzymes could be used for evaluation of abomasal tissue damage because of displacement in displaced abomasum (DA) cases. Objectives The aim of this study was to investigate the concentrations of acute-phase proteins, oxidative stress indicators, and activities of enzymes in blood serum and peritoneal fluid in cattle with right displaced abomasum (RDA) and left displaced abomasum (LDA) and in healthy cows. Animals A total of 60 Holstein Friesian cows in early lactation were used, 31 with left and 9 with right displaced abomasum without volvulus diagnosis and no other postpartum disease, and 20 healthy cows as a control. Materials and Methods DA diagnosis in dairy cows consisted of physical examination, laboratory, and specific DA tests. Acute-phase proteins, oxidative stress indicators, and enzyme activities were measured in blood serum and peritoneal fluid. Results In the RDA group, serum haptoglobin (HPG), serum amyloid A (SAA), malondialdehyde (MDA), adenosine deaminase (ADA), myeleperoxidase (MPO), aspartate aminotransferase (AST), creatine kinase (CK, creatine kinaseMB (CK-MB), and gamma-glutamyl transferase (GGT) activity increased significantly, and serum HPG, MDA, ADA, and AST concentrations increased significantly in the LDA group (P < .05). Peritoneal fluid HPG, MDA, ADA, MPO, ALP, GGT, and LDH concentrations increased significantly, whereas NO concentrations reduced significantly in the RDA group, and HPG, MDA, ADA, and TP concentrations increased significantly, whereas concentrations of NO reduced significantly in the LDA group (P < .05). Conclusions and Clinical Importance There are acute-phase responses, oxidative stress, and abomasal tissue damage because of displacement in DA cases. Especially, HPG, MDA, ADA, and MPO concentrations can provide specific information to help in understanding these changes.
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    Evaluation of the hormones responsible for the gastrointestinal motility in cattle with displacement of the abomasum; ghrelin, motilin and gastrin
    (Bmj Publishing Group, 2013) Ozturk, A. S.; Guzel, M.; Askar, T. K.; Aytekin, I.
    This study provides the evidence of increased serum gastrointestinal motility hormone concentrations including ghrelin, motilin and gastrin in cattle with displacement of abomasum (DA). In this study, 38 cows with DA (21 left DA (LDA) and 17 right DA (RDA)) and 15 healthy controls were included. All cattle with DA were at the stage of postpartum one to eight weeks, and had clinical signs including anorexia, decreased milk yield and scanty, pasty faeces. Serum ghrelin, motilin and gastrin concentrations, and leptin concentration which is a functional antagonist of ghrelin, were determined by ELISA. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), Na, K, Cl, Ca and P concentrations were measured by spectrophotometer. In serum biochemical analysis, increases were seen on the serum ALT, AST and GGT activities; however, serum Na, K, Cl and P concentrations decreased in abomasal displacement compared with the control animals. The serum ghrelin, motilin and gastrin concentrations increased in the cattle with LDA and RDA, as compared with those in the healthy controls. On the other hand, serum leptin concentration decreased in the cattle with DA compared with the controls. Increases in the serum ghrelin, motilin and gastrin concentrations might be attributed to activation of gastrointestinal motility hormones to enhance of gastric emptying in impaired gastric motility and/or outlet occlusion in displaced abomasum.
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    Frequencies of blood type A, B and AB in cats from the mediterranean sea coast of the Turkey
    (Ecole Nationale Veterinaire Toulouse, 2010) Arikan, S.; Guzel, M.; Ozturk, A. S.; Simsek, O.
    The aim of the present study was to determine the frequency of blood types in cats located in southern Turkey and the inherent risk of isoerythrolysis in kittens born to parents of unknown blood type. A total of 240 non-pedigree cats stemming from 6 cities of the Mediterranean Sea cost were blood typed by slide agglutination assays: the type A was assessed using an anti-A antiserum prepared from known type B cats, while the type B was determined using the lectin from Triticum vulgaris, then the proportions of mating carrying a risk for neonatal isoerythrolysis were calculated. The overall proportions of type A, type B and type AB were 72.1%, 25.4% and 2.5% respectively and the specific prevalence of these blood types were relatively homogeneous among the 6 cites. Considering that all type B cats possess high serum titre of anti-A antibodies, the calculated mean risk for neonatal isoerythrolysis was high (18.9%), ranged from 9.9% to 22.0% according to the cities. These results clearly demonstrate that not-typed transfusion or mating between unknown blood type cats carries a high risk for incompatibility reactions because of the relatively high type B prevalence and lead to strong recommendation for blood typing cats.