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    Detection of 'Candidatus phytoplasma pyri' in Turkey
    (Alma Mater Studiorum, Univ Bologna, 2007) Gazel, Mona; Serce, Cigdem Ulubas; Caglayan, Kadriye; Ozturk, Harun
    Typical 'Candidatus Phytoplasma pyri' (Pear Decline, PD) symptoms were first observed in 2005 in pear orchards of Bursa province, which is an important pear growing area in Turkey. The present report resulted from a survey of pear varieties to investigate the infection level of PD in Bursa in 2006. The observed symptoms were foliar reddening in late summer and fall, leaf roll, leaf curl, poor growth and slow or quick decline. The presence of PD phytoplasma in pear trees was investigated by polymerase chain reaction using universal P1/P7 and R16F2/R2 primers and the identification was performed by RFLP analyses. A total of 116 pear trees were tested and 61 samples were found infected by the phytoplasma. 'Ca. P. pyri' was identified using RsaI, SspI and MseI restriction enzymes. PD is widely distributed in pear trees with a high incidence and it is a big threat for pear production in Turkey.