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    The Distribution of Intestinal Parasites Determined Between 2009-2010 in Erciyes University, Medical Faculty, Parasitology Laboratory
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Cetinikaya, Ulfet; Yazar, Suleyman; Kuk, Salih; Ates, Serpil; Hamamci, Berna; Gedikbas, Tugba; Sahin, Izzet
    The infection caused by intestinal parasites infects through the mouth are common in the world. The intestinal parasites are a health problem not only developing countries but also developed country. They cause various symptoms such as abdominal pain, diarrhea, weight loss, anemia, and growth retardation. The prevalence of intestinal parasites is affected by various factors such as climate, socioeconomic status, age, sex, sanitation, and hygiene. In this study, we aimed the distribution of intestinal parasites in patients presenting with intestinal complaints to Erciyes University Medical Faculty, Parasitology department. This study carried out from January 2009-December 2010. A total of 16.445 stool samples were examined using native-Lugol and sedimentation methods and 1.482 cellophane tape preparations were examined directly. Totally 3.214(19.5%) patients, 1.745 (10.6%) men and 1.469 (8.9%) women were found infected with one or more intestinal parasite species. The more prevalent parasites were found as Blastocystis hominis 2.602 (15.8%), Entamoeba coli 351(2.1%) and Giardia intestinalis 299 (1.9%) respectively.
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    Öğe
    Molecular Characterization of Echinococcus granulosus Isolates Obtained From Different Hosts
    (Ankara Microbiology Soc, 2017) Erdogan, Emrah; Ozkan, Bora; Mutlu, Fatih; Karaca, Serkan; Sahin, Izzet
    Echinococcus granulosus is a parasite that can be seen throughout the world. So far, five species of genus Echinococcus have been identified as parasite in people: E.granulosus, E.multilocularis, E.vogeli, E.oligarthrus, E.shiquicus. Larval (metacestod) form of parasite settles in internal organs of hoofed animals (cattle, goats, pigs, horses, sheep, etc.) and human; the adult form is found in small intestine of final host, canine. Disease caused by parasite called as Cystic echinococcosis (CE) is an important health problem and causes economic losses in many countries including our country that livestock is common. Infective eggs cause infections in intermediate hosts by taking oral way and rarely inhalation. Received egg opens in the stomach and intestines of intermediate host and oncosphere is released. Oncosphere quickly reaches the lamina propria of the villus epithelium by its histolytic enzymes and hooks. It usually transported from here to the liver and lungs, less frequently, muscle, brain, spleen, kidney and to other organs through the veins. By molecular studies, five species have been validated taxonomically and 10 different variants or strains of E.granulosus have been identified. Host and developmental differences between strains may negatively affect control studies and fight against the parasite. This study aimed to determinate E.granulosus strains obtained from cyst material of different intermediate hosts from different regions of Turkey by molecular methods. In the study, 25 human, 8 cattle, 6 sheep and 2 goat cysts material has been collected. Total genomic DNA was isolated from protoscoleces in cyst fluid and analyzed by PCR with COX-1 (L) and COX-1 (S) genes specific primers. DNA sequence analysis for each PCR product has been made. DNA sequence analysis results evaluated phylogenetically by MEGA analyze and BLAST software. As a result of this study, all isolates were identified as E.granulosus sensu stricto (G1) by DNA sequence analysis. CE is a major public health problem for our country so we believe that obtained data from this study is an important source for parasite control, effective diagnosis, treatment techniques, eradication, vaccination and drug development. Similar studies will be beneficial to cover all other regions of Turkey and to develop effective and successful control programs.