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Öğe AC-2306327-0000001 Heavy Metals, Trace Elements and Biochemical Composition of Different Honey Produce in Turkey(Asian Journal Of Chemistry, 2009) Sahinler, Nuray; Gul, Azlz; Akyol, Ethem; Oksuz, AbdullahIn this work, the biochemical composition, trace elements and heavy metal contents of honey samples, which were collected from different regions and different botanical origin in Turkey were evaluated. The average content of minerals, moisture, acidity, hydroxymethylfurfural, diastase, invert sugar, pH, sucrose, electrical conductivity and heavy metal contents were determined, The biochemical composition of different botanical originated honeys were determined within the limits of Turkish Standard Institution (TSE), CODEX and EU standards. Level of invert sugar in cotton honey and sucrose level in sunflower honey weren't found agree with TSE and EU standards. In addition to biochemical compositions, the presence of Al, Ba, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb and Zn were determined agree with ICP AES, The highest value of Cr, Cu, K, Mn, Na and Al, contents were found in pine honeydew honey with a levels of 0.50, 2.14, 1931, 1.46, 285, 7.49 mg kg(-1), respectively. The highest level of Cd, Co, Mg, Ni and Pb (0.32, 0.22, 103, 0.64, 1.29 mg kg) were found in cotton honey. The highest level of Ba and Zn were determined in multifloral honey with a level of 1.47 ppm and 3.29 ppm, respectively. K, Na and Mg were the major macro elements in all honey samples. This study showed that the source of nectar and ecological regions have an important effect on the biochemical compositions, trace elements and heavy metals contents of honey.Öğe Chemical Composition and Preservative Effect of Thrkish Propolis on Egg Quality Durig Storage(Asian Journal Of Chemistry, 2009) Sahinler, Nuray; Gul, Aziz; Copur, GuelsenThe present study was carried out to investigate chemical composition and preservative effects of Turkish propolis on quality of fresh eggs. For this purpose, different concentrations of ethonolic extract of propolis (5, 8, 10 % EEP) were prepared. Total of (9 period x 5 group x 10 n) 450 egg were used during experiment. The eggs were randomly divided into 5 experimental groups. The first, second and third group eggs covered with 5, 8, 10 % EEP, fourth group with alcohol control and fifth group as a control (uncovered), respectively. Ten eggs in each group were drawn randomly and examined each week between 17 March-12 May 2006. Chemical analysis of propolis extracts indicated that the propolis samples had high concentrations of the aromatic acids, esters and other derivatives which are responsible for the antibacterial, antifungal, antiviral, anti inflammatory and anticancer properties of propolis such as benzyl cinnamate, methyl cinnamate, caffeic acid, cinnamyl cinnamate and cinnamoylglcine besides the most common compounds as fatty acid, terpenoids, esters, alcohols hydrocarbons and aromatic acids. It was found that ethanolic extract of propolis improved interior egg quality.Öğe Chemical composition and preservative effect of Turkish propolis on egg quality durig storage(Chemical Publishing Co., 2009) Sahinler, Nuray; Gül, Aziz; Çopur, GülsenThe present study was carried out to investigate, chemical composition and preservative effects of Turkish propolis on quality of fresh eggs. For this purpose, different concentrations of ethonolic extract of propolis (5, 8, 10 % EEP) were prepared. Total of (9 period × 5 group × 10 n) 450 egg were used during experiment. The eggs were randomly divided into 5 experimental groups. The first, second and thi?j group eggs covered with 5,8,10 % EEP, fourth group with alcohol control and fifth group as a control (uncovered), respectively. Ten eggs in each group were drawn randomly and examined each week between 17 March-12 May 2006. Chemical analysis of propolis extracts indicated that the propolis samples had high concentrations of the aromatic acids, esters and other derivatives which are responsible for the antibacterial, antifungal, anti- viral, antiinflammatory and anticancer properties of propolis such as benzyl cinnamate. methyl cinnamate, caffeic acid, cinnamyl cinnamate and cinnamoylglcine besides the most common compounds as fatty acid, terpenoids, esters, alcohols hydrocarbons and aromatic acids. It was found that ethanolic extract of propolis improved interior egg quality.Öğe Determiming the Performances of Honeybees, Pure Bred Caucasian, Anatolian and Their Reciprocal Crosses under Nomad Beekeeping Conditions(Medwell Online, 2009) Yeninar, Halil; Akyol, Ethem; Sahinler, NurayThis study was carried out to determine the brood production, development of adult worker bee population and honey yield of pure bred Caucasian (Apis mellifera Caucasica) (C (female male)), Anatolian (A (female male) (Apis mellifera anatoliaca) honeybees and their reciprocal crosses (C (male) x A (female) and A (female) x C (male)) under nomad beekeeping conditions in Central and South-East Anatolia with coastal side of East Mediterranean region of Turkey. All Queens were reared in same apiary, with same methods and at the same time. All of them were instrumentally inseminated. Average number of frames, covered with bees, of 10 measuring times between 25th April and 31st October, in C (female male), A (female male), C (female) x A (male) and A (female) x C (male) genotypes were found to be 11.6 +/- 0.4, 17.2 +/- 0.9, 11.5 +/- 0.5 and 17.8 +/- 1.0 number/colony and the brood areas 3754.2 +/- 340.8, 5425.1 +/- 416.9, 3742.6 +/- 323.8 and 5194.8 +/- 428.7 cm(2) colony(-1) were found, respectively. The total average honey yields for 4 groups were found as 36.3 +/- 3.5; 43.9 +/- 4.1; 33.1 +/- 3.5 and 55.3 +/- 4.5 kg colony(-1), respectively in 3 different ecological regions. There were found significant differences (p<0.01) among the groups with respect to on frames covered with bees, brood area and the honey yields. There were calculated high and significant (p<0.01) correlations (r = 0.85, r = 0.82) between frames covered with bees, brood areas and honey yield.Öğe Determination of Honey Botanical Origin by Using Discriminant Analysis(Medwell Online, 2009) Sahinler, Suat; Sahinler, Nuray; Gul, AzizOne of the most important criterions is determination of honey botanical origin in point of the sense of taste, the force of habit and quality of honey and the results of this, in preference of honey. The purpose of this study, is to discriminate the honey type using a linear discriminant function on the basis of honey chemical characteristics by using discriminant analysis, which is one of the multivariate techniques and has recently been much used in applied sciences. In this study, independent variables, mineral content (%), moisture (%), pH, acidity (meq), invert sugar (%), sucrose (%), diastase level and HMF (mg) belonging to 5 types as sunflower, citrus, cotton, pine and high plateau honeys were used. The discriminant functions from different combinations of variables on the basis of honey chemical characteristics were given and the cases from the original honey botanical origins, sunflower, citrus, cotton, pine and high plateau, could be classified 100% correctly by using these functions.Öğe The effect of propolis egg shell coatings on interior egg quality(Eugen Ulmer Gmbh Co, 2008) Copur, Gulsen; Camcil, O.; Sahinler, Nuray; Gul, A.Effects of various concentrations of propolis for egg coating (5%, 8% and 10% of propolis in ethanol) on the interior quality of fresh eggs were evaluated during 4 weeks of storage. During storage, albumen height decreased whereas albumen pH increased. The albumen pH of the uncoated eggs (control; Group I), and the eggs coated with alcohol (Group II) and 5% propolis (Group III) was significantly higher (P 0.05) than the albumen pH of eggs coated with 8% (Group IV) and 10% propolis (Group V). On the other hand, at 4 weeks storage eggs of Groups IV and V had a higher albumen index than the rest of the groups. The HU value of eggs of Groups IV and V were significantly higher than for eggs of Groups I, II and III. Coating with 10% propolis (Group V) resulted in the maintenance of grade 'A' for 2 weeks longer than for the other groups. Propolis did not affect yolk-index (YI) value (p > 0.05). In conclusion, coating of eggs with 10% propolis extract improved interior egg quality during storage.Öğe The effect of propolisis and mesalazine on bacterial translocation in an experimental colitis model(Saudi Med J, 2007) Aslan, Ahmet; Cetin, Meryem; Temiz, Muhyittin; Sahinler, Nuray; Besirov, Elmir; Aban, Nedim[Abstract Not Available]Öğe Effectiveness of mesalamine and propolis in experimental colitis(Springer, 2007) Aslan, Ahmet; Temiz, Muhyittin; Atik, Esin; Polat, Gurbuz; Sahinler, Nuray; Besirov, Eimir; Aban, NedimThis study was conducted to investigate the effects of propolis and mesalamine on experimental colitis in rats. Distal colitis was induced in rats by intracolonic instillation of 2 mL of 4% acetic acid. The animals were randomly assigned to 5 groups: group 1, control, (n=8); group 2, colitis, received no treatment (n=8); group 3, colitis+mesalamine, 2 mL once a day via an enema (n=8); group 4, colitis+propolis, 600 mg/kg once a day via intragastric lavage (n=8); and group 5, colitis+ mesalamine+propolis for 1 wk (n=8). Levels of nitric oxide were statistically significantly different in comparisons between groups 1 and 2, groups 2 and 3, and groups 4 and 5. Malondialdehyde levels were significantly different when group 2 was compared with groups 3, 4, and 5. A significant difference was observed when group 3 was compared with group 4 for myeloperoxidase. Most propolis-treated rats had normal histology; mesalamine-treated and propolis+mesalamine-treated rats had inflammatory cell infiltration at rates of 50% and 33%, respectively. The investigators concluded that propolis and mesalamine are efficient independently and in combination, but that their combined effect was not observed to be additive in experimental colitis.Öğe The effects of additive feeding and feed additives before wintering on honey bee colony performances, wintering abilities and survival rates at the East Mediterranean region(2006) Akyol, Ethem; Yeninar, Halil; Sahinler, Nuray; Guler, AhmetIn this study; the effects of additive feeding with vitamin, fumagillin and pollen supplement on survival rates, wintering abilities, amount of adult bees and brood production were examined at subtropical temperate region before wintering. The wintering ability, survival rates, adult bees and brood areas were positively affected by feeding and feed additives. The lowest values of research components were observed on unfed control groups colonies. © 2006 Asian Network for Scientific Information.Öğe Effects of diluents and plasma on honey bee (Apis mellifera L.) drone frozen-thawed semen fertility(Elsevier Science Inc, 2017) Gul, Aziz; Sahinler, Nuray; Onal, Ali G.; Hopkins, Brandon K.; Sheppard, Walter S.Cryopreservation is an advanced method used to protect germplasm in liquid nitrogen. Honey bees are of special interest to protect because of their pollination activity and critical role in agriculture. There has been important progress in the cryopreservation of honey bee germplasm in recent years, leading to practical recovery of genetic material for breeding purposes following freezing. However, there remains room for improvement and the goal of the present study was to evaluate the effect of different extenders added post-thaw on the fertilization rate of cryopreserved honey bee semen. The purpose of adding extender post-thaw was to dilute the cryoprotectant to remove chemicals after centrifugation because of potential adverse effects. The control consisted of frozen-thawed semen without the addition of an extender; treatment groups included the addition of one of the following extenders: glucose solution, fresh ram semen plasma, fresh honey bee semen plasma, extender solution. All of the above treatments and frozen-thawed control were compared to fresh semen. For each group, 15 virgin queens were instrumentally inseminated with the semen-diluent solution and introduced into nucleus colonies to determine the brood patterns of the queens. Percentages of worker brood produced in the fresh semen, frozen-thawed semen control, glucose, fresh ram semen plasma, fresh honey bee semen plasma, and extender solution supplemented groups were 98 +/- 1.1%, 47 +/- 0.9%, 3.0 +/- 0.8%, 0.3 +/- 0.1%, 48.1 +/- 4.1% and 40.3 +/- 2.4%, respectively. Similiarly, spermatozoa numbers in the spermathecae of the same treatment groups were 3.6 x 10(6),1.6 x 10(6), 73 x 10(6), 4.7 x 10(6), 8.1 x 10(6), and 4.6 x 10(6) spermatozoa for the same treatment, respectively. The differences in both worker brood percentage and sperm count in the spermatheca were statistically significant (P < 0.01) among all treatment groups, except the frozen thawed control group and fresh drone semen plasma group. We found a positive correlation between sperm count in the spermatheca and the percentage of worker brood (r = 0.91). With the exception of fresh honey bee semen plasma, the fertility rate was reduced following the addition of various plasmas and diluents post-freezing. (C) 2017 Elsevier Inc. All rights reserved.Öğe Effects of heating and storage on hydroxy methylfurfural and diastase activity of different Turkish honeys(Int Bee Research Assoc, 2007) Sahinler, NuraySamples were taken of Turkish honeys of different botanical origin, including Citrus (Citrus spp.), a high plateau mixture of Medicago sativa, Onobrychis sativa, astragalus globosus L., Echinops ritr L., Trifolium montanum L., Thymus vulgaris, and Verbascum densiflorum, cotton (Gossypium herbaceum), and pine (Pinus nigra) (honeydew honey). Samples were heated at 55, 65 and 75 degrees C for different heat exposure periods (15, 30,45,60 min). Honey samples were then left at room temperature (21 degrees C) for 9 months after which period the changes in diastase activity and hydroxyl methylfurfural (HMF) content were observed. Diastase number and HMF content of honey samples were significantly affected by heating, heat exposure period and storage time.Öğe The effects of hive types (shield and sword) on wintering ability, survival rates and strength of honeybee colonies (A. mellifera L.) in spring season(Springer, 2010) Yeninar, Halil; Akyol, Etem; Sahinler, NurayThis study was carried out to determine the effects of shield and sword comb orientation hive types on wintering ability, survival rates (in winter) and population growth of honeybee colonies (A. mellifera anatoliaca) in spring season. In ancient Anatolia beekeeping; honeybee colonies were identified sword and shield (the colonies which build up the combs vertical and horizontal according to positions of the hive entrance) before the uses of top-opened hive with movable frames. Total twenty honeybee colonies, which have similar condition according to queen age, genotype, number of frames covered with adult worker bees, brood areas and food stocks, were used in this study. Average wintering ability of colonies in the shield and sword groups were found to be 98.57% and 69.76%; average survival rates were found to be 100% and 100% in shield and sword group colonies respectively. The average number of frames covered with adult worker bees at mid June in shield and sword group colonies were found to be 15.6 +/- 1.58, 12.00 +/- 1.25 number/colony and the average brood areas were found as 7863.5 +/- 402.9, 5997.0 +/- 373.3 cm(2)/colony respectively. Differences between the group means on wintering ability, sealed brood areas and colony strength were found significant (P < 0.01), but differences on survival rates were not found significant (P > 0.05). The colonies living in shield (horizontal) hives have showed better wintering ability and more colony population than colonies living in sword (vertical) hives.Öğe Effects of propolis and pollen supplementations on growth performance and body components of Japanese quails ( Coturnix coturnixjaponica)(2009) Canogullari, Sibel; Baylan, M.; Sahinler, Nuray; Sahin, A.The present study was carried out to investigate the effects of honey bee propolis and pollen supplementation on growth performance and body components in quail (Coturnix coturnix japonica). Two experiments were conducted. In the first experiment, propolis ethanolic extract (30%, PEE) was supplemented in diets at levels of 0, 5 and 10 ml PEE kg 1 while in the second experiment, pollen was supplemented in diets at four levels (0, 5, 10 and 20 g pollen kg-1). In both experiments, chicks were fed with diet containing 240 g crude protein and 3100 kcal ME per kg diet. During the experimental period, body weight, feed consumption and feed efficiency were determined weekly. At the end of the experiments, 3 female and 3 male quail from each subgroup were killed humanely to determine body components. Experimental results showed that supplementation of PEE and pollen did not significantly affect body weight gain, feed efficiency or body components (P > 0.05). It was concluded that propolis and pollen had no effect at the levels investigated on performance and body components of quail. © Verlag Eugen Ulmer, Stuttgart.Öğe Effects of propolis and pollen supplementations on growth performance and body components of Japanese quails (Coturnix coturnix japonica)(Eugen Ulmer Gmbh Co, 2009) Canogullari, Sibel; Baylan, M.; Sahinler, Nuray; Sahin, A.The present study was carried out to investigate the effects of honey bee propolis and pollen supplementation on growth performance and body components in quail (Coturnix coturnix japonica). Two experiments were conducted. In the first experiment, propolis ethanolic extract (30%, PEE) was supplemented in diets at levels of 0, 5 and 10 ml PEE kg(-1) while in the second experiment, pollen was supplemented in diets at four levels (0, 5, 10 and 20 g pollen kg-1). In both experiments, chicks were fed with diet containing 240 g crude protein and 3100 kcal ME per kg diet. During the experimental period, body weight, feed consumption and feed efficiency were determined weekly. At the end of the experiments, 3 female and 3 male quail from each subgroup were killed humanely to determine body components. Experimental results showed that supplementation of PEE and pollen did not significantly affect body weight gain, feed efficiency or body components (P > 0.05). It was concluded that propolis and pollen had no effect at the levels investigated on performance and body components of quail.Öğe Effects of Propolis on Storage of Sweet Cherry Cultivar Aksehir Napolyon(Asian Journal Of Chemistry, 2009) Candir, Elif Ertuerk; Ozdemir, Ahmet Erhan; Soylu, Emine Mine; Sahinler, Nuray; Gul, AzizSweet cherries cultivar Aksehir Napolyon were dipped in ethanol extracted propolis (EEP) and water extracted propolis (WEP) in various concentrations immediately after harvest and then kept at 0 degrees C, 85-90 % relative humidity for 4 weeks, The following treatments were applied to the fruits: dipping in water, dipping in ethanol (70 %), dipping in 1, 5 and 10 % concentrations of ethanol extracted propolis and dipping in 1, 5 and 10 % concentrations of water extracted propolis. The effects of propolis on incidence of fungal decay and fruit quality (weight loss, total soluble solids, titratable acidity, skin colour, stem browning, surface pitting, appearance and taste) were assessed at weekly intervals during storage. Ethanol extracted propolis treatments were effective in preventing fungal decay in cherries for 4 weeks, but adversely affected sensory quality and stem colour of cherries.Öğe Heavy metals, trace elements and biochemical composition of different honey produce in Turkey(Chemical Publishing Co., 2009) Sahinler, Nuray; Gül, Aziz; Akyoli, Ethem; Öksüz, AbdullahIn this work, the biochemical composition, trace elements and heavy metal contents of honey samples, which were collected from different regions and different botanical origin in Turkey were evaluated. The average content of minerals, moisture, acidity, hydroxymethylfurfural, diastase, invert sugar, pH, sucrose, electrical conductivity and heavy metal contents were determined. The biochemical composition of different botanical originated honeys were determined within the limits of Turkish Standard Institution (TSE), CODEX and EU standards. Level of invert sugar in cotton honey and sucrose level in sunflower honey weren't found agree with TSE and EU standards. In addition to bio- chemical compositions, the presence of Al, Ba, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb and Zn were determined agree with ICP AES. The highest value of Cr, Cu, K, Mn, Na and Al, contents were found in pine honeydew honey with a levels of 0.50,2.14,1931,1.46,285,7.49 mg kg"1, respectively. The highest level of Cd, Co, Mg, Ni and Pb (0.32, 0.22,103,0.64,1.29 mg kg -1) were found in cotton honey. The highest level of Ba and Zn were determined in multifloral honey with a level of 1.47 ppm and 3.29 ppm, respectively. K, Na and Mg were the major macro elements in all honey samples. This study showed that the source of nectar and ecological regions have an important effect on the bio- chemical compositions, trace elements and heavy metals contents of honey.
