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Öğe Determination of the incidence of embryonic mortality in synchronized ewes during the breeding season(Csiro Publishing, 2007) Erdem, H.; Saribay, M. K.; Tekeli, T.[Abstract Not Available]Öğe The Effect of PMSG Treatment on Pregnancy Rates in Synchronized Heifers using Norgestomet Ear Implants(Israel Veterinary Medical Assoc, 2018) Nergiz, K. B.; Saribay, M. K.This study was carried out in heifers, which were administered with norgestomet implants for estrus synchronization, with an aim to determine the effect of PMSG, administered to the animals on the day of the removal of the implants, on pregnancy rates. A hundred Holstein heifers, aged 15-16 months, constituted the material of the study. In this study all of the heifers were administered ear implants containing 3 mg norgestomet subcutaneously on the external surface of the ear. Following the insertion of the implants, on the same day, all of the heifers were intramuscularly administered 10 mu g buserelin acetate. After a period of 10 days, the implants were removed. Two days prior to the removal of the implants, the heifers were injected with 500 mu g of cloprostenol by intramuscular route. On the day of the removal of the implants, the animals were randomly assigned to two groups which comprised an equal number of heifers. The heifers included in Group I (n=50) were injected with 400 IU pregnant mare's serum gonadotropin (PMSG) by intramuscular route, whilst the heifers included in Group II (n=50) were not subjected to any treatment. At the 56th hour following the removal of the implants, all heifers were inseminated regardless of the presence of oestrus signs. The pregnancy diagnosis was carried out on 35th days after insemination. The pregnancy rates achieved in Groups I and II were determined as 86% (43/50) and 54% (27/50), respectively (P<0.001). It was concluded that, in heifers, which were synchronized with subcutaneous norgestomet implants, the addition of PMSG on the day of the removal of the implants increased the pregnancy rates.Öğe Estimation of periostin as a biomarker for early pregnancy diagnosis in goats: a preliminary study(Shiraz Univ, 2021) Kose, A. M.; Saribay, M. K.; Urer, Koldas E.; Naseer, Z.; Dogruer, G.; Karaca, F.; Cetin, Coskun N.Background: Periostin (POSTN) is an extracellular matrix (ECM) protein that plays an important role in the metastatic process and cancer cell migration. As implantation is a similar mechanism to metastasis, it has been hypothesized that POSTN may also play a role in the implantation process. Aims: The aim of the present study was to compare POSTN and progesterone levels during the early pregnancy stage in Damascus goats. Methods: Forty goats were synchronized using progesterone based sponges and were mated upon estrus signs display. While ten goats were kept as control (CON) and were not allowed to mate. Blood samples were taken through jugular venepuncture from CON and synchronized goats on day 13, 15, 17, 19, and 21 of breeding. Progesterone and POSTN levels were determined by enzyme-linked immunosorbent assay (ELISA). Later the pregnancy diagnosis was confirmed by transabdominal ultrasonography on day 50 after mating. Results: Progesterone level was influenced by status of pregnancy and day of observation with an interaction between the status of pregnancy and day of observation in goats. Whereas POSTN level was only affected by the day of observation. Conclusion: POSTN level did not vary with progesterone level during phase of embryonic implantation in goats; however, standardization and application of different procedures for POSTN assay in a large group of animals might be useful as an early pregnancy biomarker in goats.Öğe Estrus synchronization during transition period, timed artificial insemination (TAI) and the effect of GnRH administration at the TAI on fertility in lactating goats(Wiley-Blackwell, 2011) Saribay, M. K.; Karaca, F.; Dogruer, G.; Ates, C.[Abstract Not Available]Öğe Evaluation of blood omega-3 and omega-6 levels in healthy female dogs and female dogs with mammary tumours(Hellenic Veterinary Medical Soc, 2021) Tuzlu, T.; Saribay, M. K.; Urer, E. Koldas; Kose, A. M.; Gozer, A.; Yakan, A.; Ozsoy, S. Y.The study was designed to determine the blood levels of omega fatty acids, which have an important role in the etiology of mammary tumours, in healthy and female dogs with mammary tumour. The study was carried out in 9 female dogs with histologically confirmed mammary tumour and a control group with 9 healthy female dogs without clinical mammary neoplasia. 10 ml cephalic blood samples were collected by using a 21G x 1.5.. blood collecting needle into anticoagulated tubes before the surgical removing of the mammary masses. Mastectomy was performed in all female dogs with mammary tumours and all the mammary specimens were sent to laboratory for histopathological examination. According to histopathological diagnosis results, all of the tumours were found to be malignant. Omega-3 levels were found to be higher in healthy female dogs (p<0.001) whereas omega-6 levels were higher in female dogs with mammary tumour (p<0.001). These observations support the notion tha thigh levels of omega-3 fatty acids might proove to have a protective role on mammary tumor formation in female dogs, while increased levels of omega-6 fatty acids may be related to an increased mammary tumor risk. This difference between omega-3 and omega-6 levels was found to be caused mainly by Eicosatrienoic acid. It is concluded that omega fatty acids mayplay an important role in the biological mechanism of mammary tumour in female dogs.Öğe Prevention of embryonic death using different treatment procedures in ewes(Wiley-Blackwell Publishing, Inc, 2006) Ataman, M. B.; Akoz, M.; Saribay, M. K.; Erdem, H.[Abstract Not Available]Öğe Variations in serum metabolites in response to season, cyclicity, and pregnancy in estrus-synchronized Damascus goats(Springer, 2020) Saribay, M. K.; Naseer, Z.; Dogruer, G.; Ozsoy, B.; Ates, C. T.The current study was carried out to determine the changes in serum electrolytes, liver enzymes, and hormones during breeding and non-breeding seasons in estrus-synchronized goats. Forty goats (n = 40) were synchronized by inserting the sponges intravaginally for 12 days; PMSG and PGF(2)alpha were injected on day of sponge removal. Blood samples were collected from the goats at the time of sponges insertion (day -12), estrus day (day 0), and at 15 day after sponge withdrawal (day 15). The blood samples were analyzed for glucose, cholesterol (CHO), aspartate aminotransferase (AST), alanine aminotransferase (ALT), thyroid stimulating hormone (TSH), triiodothyronine (T-3), thyroxine (T-4) and progesterone (P-4), calcium (Ca), phosphate (PO4), magnesium (Mg), sodium (Na), potassium (K), and chlorine (Cl) levels. Repeated measurements ANOVA of general linear model were used as statistical model. The results showed that seasons have an effect (p < 0.05) on glucose, Ca, Na, K, Cl, CHO, T-3, and T-4 levels. There was an effect (p < 0.05) of cyclicity only on P-4 level. Whereas, nonpregnant, or goats carrying singlet and twin fetuses have shown variation (p < 0.05) in glucose, Ca, and P-4 levels. Although, the correlations existed between different serum metabolites but strong correlations (p < 0.05) were observed either between Ca and Na or T-3 and T-4. In conclusion, the serum electrolytes and liver enzymes are correlated with hormones during the different seasons, pregnancy status, and fecundity. In recommendation, the results of the study could be as managemental tool to monitor the reproductive activity across seasons and to maintain pregnancy carrying twining in goat breeds.
