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    First dose optimization study on freezing Anatolian buffalo semen
    (Ankara Univ, 2024) Bastan, Ilktan; Sahin, Derya; Korkmaz, Firat; Simsek, Seher; Kaya, Ufuk; Satilmis, Muharrem
    The main objective of sperm production centers is to produce as many straws as possible from the obtained ejaculates using the optimal dilution rate. To this end, this study is the first to evaluate the effect of different semen extender rates on Anatolian buffalo semen quality. Ejaculates were collected by artificial vagina from three Anatolian buffalo bulls. These ejaculates were divided into three aliquots and filled into 0.25 ml straws with soy-based extenders at concentrations of 35, 25, and 15 million sperm/straw (n=105). The straw samples of different sperm concentrations were frozen. The quality of sperm was evaluated after thawing (37 degrees C, 30 sec) and following the thermoresistance test (37 degrees C, 3 h). The post-thaw total motility and progressive motility values were similar between the groups. However, following the thermoresistance test, there was a significant decrease in total motility in the 35 million sperm/straw group, and the progressive motility was significantly higher in the 25 million sperm/straw group. There was no statistically significant difference between the groups in terms of sperm kinetic parameters, except for VSL after thawing, as well as VAP and LIN values following the thermoresistance test. The overall mean PMAI and STR values were the highest in the 25 million sperm/straw group. In conclusion, it is recommended to dilute the Anatolian buffalo semen at a concentration of 25 million/0.25 ml when freezing it with a soy-based semen extender. In addition, it is considered that soy-based extenders compensate for cryo-damage to sperm motility for a short time, and the thermoresistance test should be applied for objective evaluation in dose optimization studies.
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    Investigation on the Hair Characteristics of Damascus and Kilis Goats
    (Taylor & Francis Inc, 2022) Behrem, Sedat; Keskin, Mahmut; Gul, Sabri; Unay, Engin; Satilmis, Muharrem; Unal, Murat; Sacli, Yurdakul
    This study aimed to investigate the coarse outer guard hair and the fine down hair (cashmere) characteristics of Kilis and Damascus goats and to evaluate their potential for industrial use. Yearling goats, goats at first birth, second birth, and third birth were used as animal materials from Gaziantep. From the shoulder, rump, and rib areas of each goat, 20-25 g of the guard hair and the fine down hair (cashmere) samples were collected. These samples were examined in terms of diameter, length, tenacity, and elasticity at the Fleece and Mohair Laboratory of the International Livestock Research and Training Center. In the study, the average fiber diameter values were determined as 15.0 +/- 0.08 and 15.1 +/- 0.07 microns, and the fiber length values were 23.4 +/- 0.38 and 22.4 +/- 0.34 mm for Kilis and Damascus goats, respectively. In the light of these results, it can be said that the cashmere fiber diameter for both breeds is suitable for the textile industry. On the other hand, the fiber length is not long enough for the textile industry but can be evaluated by hand weaving method.
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    Reaction time as a libido indicator and its relation to pre-freeze and post-thaw sperm quality in bulls
    (Wiley, 2023) Korkmaz, Firat; Bastan, Ilktan; Sahin, Derya; Simsek, Seher; Kaya, Ufuk; Satilmis, Muharrem
    Libido and sperm quality output relationship is already not clear in farm animals. The present study compared reaction time (RT) as a libido indicator and the pre-freeze and post-thaw sperm quality of AI bulls. Before the collection of ejaculates (n = 53, from 22 AI bulls [4.2 +/- 1 years of age]), RTs were collected using a chronometer as the interval between the bull's arrival at the semen collection area and his first false mount (FM) on another male. The ejaculates were examined for their volume, concentration and motility. Subsequently, all aliquots were diluted with a commercial semen extender and equilibrated for 3 h before freezing. Frozen semen samples were thawed and examined for sperm kinematics using CASA, plasma membrane and acrosome integrity of sperm (PMAI) by flow cytometry. Additionally, the temperature humidity index (THI) values were assessed during the study. Multiple linear regression analysis was used to analyse the data. The results indicated that THI had a significant effect on libido (p < .001). However, libido had no effect on either pre- or post-thaw sperm quality parameters except for the velocity of the average pathway (VAP) (p < .05). Therefore, relying solely on RT -libido- as an indicator of bull sperm quality at AI stations may not be reliable, as it is a complex behavioural assessment.